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Mouse Anti-CRTC2 Recombinant Antibody (CBFYC-2241) (CBMAB-C2311-FY)


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Summary

Host Animal
Mouse
Specificity
Human
Clone
CBFYC-2241
Antibody Isotype
IgG2b
Application
WB

Basic Information

Immunogen
Recombinant corresponding to human TORC2 expressed in E. coli (Q53ET0)
Specificity
Human
Antibody Isotype
IgG2b
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Lyophilized
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
CREB Regulated Transcription Coactivator 2
Introduction
CRTC2 (CREB Regulated Transcription Coactivator 2) is a Protein Coding gene. Diseases associated with CRTC2 include Human T-Cell Leukemia Virus Type 1 and T-Cell Leukemia. Among its related pathways are Metformin Pathway, Pharmacodynamics and Organelle biogenesis and maintenance. Gene Ontology (GO) annotations related to this gene include cAMP response element binding protein binding. An important paralog of this gene is CRTC1.
Entrez Gene ID
200186
UniProt ID
Q53ET0
Alternative Names
CREB Regulated Transcription Coactivator 2; TORC-2; TORC2; Transducer Of Regulated CAMP Response Element-Binding Protein (CREB) 2; Transducer Of Regulated CAMP Response Element-Binding Protein 2; CREB-Regulated Transcription Coactivator 2; Transducer Of CREB Protein 2
Function
Transcriptional coactivator for CREB1 which activates transcription through both consensus and variant cAMP response element (CRE) sites. Acts as a coactivator, in the SIK/TORC signaling pathway, being active when dephosphorylated and acts independently of CREB1 'Ser-133' phosphorylation. Enhances the interaction of CREB1 with TAF4. Regulates gluconeogenesis as a component of the LKB1/AMPK/TORC2 signaling pathway. Regulates the expression of specific genes such as the steroidogenic gene, StAR. Potent coactivator of PPARGC1A and inducer of mitochondrial biogenesis in muscle cells. Also coactivator for TAX activation of the human T-cell leukemia virus type 1 (HTLV-1) long terminal repeats (LTR).
Biological Process
Gluconeogenesis Source: UniProtKB
Glucose homeostasis Source: UniProtKB
Histone H3-K9 acetylation Source: Ensembl
Positive regulation of CREB transcription factor activity Source: UniProtKB
Positive regulation of transcription by RNA polymerase II Source: GO_Central
Protein homotetramerization Source: InterPro
Toxin transport Source: Ensembl
Viral process Source: UniProtKB-KW
Cellular Location
Cytoplasm; Nucleus. Translocated from the nucleus to the cytoplasm on interaction of the phosphorylated form with 14-3-3 protein (PubMed:15454081). In response to cAMP levels and glucagon, relocated to the nucleus (PubMed:15454081).
PTM
Phosphorylation/dephosphorylation states of Ser-171 are required for regulating transduction of CREB activity (PubMed:15589160, PubMed:17210223). CRTCs/TORCs are inactive when phosphorylated, and active when dephosphorylated at this site (PubMed:17210223). This primary site of phosphorylation, is regulated by cAMP and calcium levels and is dependent on the phosphorylation of SIKs (SIK1 and SIK2) by LKB1 (PubMed:15454081, PubMed:16817901). Following adenylyl cyclase activation, dephosphorylated at Ser-171 by PPP3CA/calcineurin A resulting in CRTC2 dissociation from 14-3-3 proteins and PPP3CA (By similarity). Both insulin and AMPK increase this phosphorylation of CRTC2 while glucagon suppresses it (PubMed:15454081). Phosphorylation at Ser-274 by MARK2 is induced under low glucose conditions and dephosphorylated in response to glucose influx (PubMed:18626018). Phosphorylation at Ser-274 promotes interaction with 14-3-3 proteins and translocation to the cytoplasm (PubMed:18626018).
Asymmetric dimethylation of arginine resisues by PRMT6 enhances the association of CRTC2 with CREB on the promoters of gluconeogenic genes.
More Infomation

Hua, X., Sun, D. Y., Zhang, W. J., Fu, J. T., Tong, J., Sun, S. J., ... & Wang, P. (2021). P7C3‐A20 alleviates fatty liver by shaping gut microbiota and inducing FGF21/FGF1, via the AMP‐activated protein kinase/CREB regulated transcription coactivator 2 pathway. British Journal of Pharmacology, 178(10), 2111-2130.

Ma, L., Chen, S., Wang, Z., Guo, S., Zhao, J., Yi, D., ... & Cen, S. (2021). The CREB Regulated Transcription Coactivator 2 Suppresses HIV-1 Transcription by Preventing RNA Pol II from Binding to HIV-1 LTR. Virologica Sinica, 36(4), 796-809.

Qiu, L., Wang, H., Dong, T., Huang, J., Li, T., Ren, H., ... & Wang, S. (2021). Perfluorooctane sulfonate (PFOS) disrupts testosterone biosynthesis via CREB/CRTC2/StAR signaling pathway in Leydig cells. Toxicology, 449, 152663.

Zhu, X., Li, H., Wu, Y., Zhou, J., Yang, G., Wang, W., ... & Ye, S. (2019). CREB‐upregulated lncRNA MEG3 promotes hepatic gluconeogenesis by regulating miR‐302a‐3p‐CRTC2 axis. Journal of cellular biochemistry, 120(3), 4192-4202.

Li, P., Zhou, C., Li, X., Yu, M., Li, M., & Gao, X. (2019). CRTC2 is a key mediator of amino acid-induced milk fat synthesis in mammary epithelial cells. Journal of agricultural and food chemistry, 67(37), 10513-10520.

Rodón, L., Svensson, R. U., Wiater, E., Chun, M. G. H., Tsai, W. W., Eichner, L. J., ... & Montminy, M. (2019). The CREB coactivator CRTC2 promotes oncogenesis in LKB1-mutant non–small cell lung cancer. Science advances, 5(7), eaaw6455.

Lee, J. H., Wen, X., Cho, H., & Koo, S. H. (2018). CREB/CRTC2 controls GLP‐1‐dependent regulation of glucose homeostasis. The FASEB Journal, 32(3), 1566-1578.

Zhang, X., Luo, Y., Wang, C., Ding, X., Yang, X., Wu, D., ... & Liu, M. (2018). Adipose mTORC1 suppresses prostaglandin signaling and beige adipogenesis via the CRTC2-COX-2 pathway. Cell reports, 24(12), 3180-3193.

Song, Y., Zhai, L., Swain, J. V., Chen, Y., Wang, P., Chen, L., ... & Xiang, S. (2018). Structural Insights into the CRTC2–CREB Complex Assembly on CRE. Journal of molecular biology, 430(13), 1926-1939.

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For research use only. Not intended for any clinical use.

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