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Mouse Anti-CORO1C Recombinant Antibody (CBFYC-2106) (V2LY-1206-LY814)


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Published Data
V2LY-1206-LY814-1
Mouse Anti-CORO1C Recombinant Antibody (CBFYC-2106) (V2LY-1206-LY814) in WB
Western blot analysis of immunoprecipitation (IP) experiments with GFP antibody in RAW-D cells expressing GFP protein only (Mock), Rab44 tagged with GFP (WT), a constitutively active (CA) mutant (Q596L), and a dominantly negative (DN) mutant (T551N). Cell lysates or IP samples using a GFP antibody were subjected to SDS-PAGE followed by Western blotting with antibodies against Coronin1c, Rab44, GFP, and GAPDH. GAPDH was used as the loading control. ...View More
Yamaguchi, Y., Kadowaki, T., Aibara, N., Ohyama, K., Okamoto, K., Sakai, E., & Tsukuba, T. (2022). Coronin1C is a GDP-specific Rab44 effector that controls osteoclast formation by regulating cell motility in macrophages. International journal of molecular sciences, 23(12), 6619.
Distributed under Open Access license CC BY 4.0, without modification.
V2LY-1206-LY814-2
Mouse Anti-CORO1C Recombinant Antibody (CBFYC-2106) (V2LY-1206-LY814) in IF
Detection of proximity ligation signals between Rab44 and Coronin1C in RAW-D cells using a confocal microscope. GFP proteins in Rab44 (WT, CA, DN) with GFP tag expressing cells were fluorescently detected as green, rolling circle amplification (RCA) products of Rab44 and Coronin1C were detected as magenta, and DAPI was detected as Blue (nuclei). Bar: 50 μm. ...View More
Yamaguchi, Y., Kadowaki, T., Aibara, N., Ohyama, K., Okamoto, K., Sakai, E., & Tsukuba, T. (2022). Coronin1C is a GDP-specific Rab44 effector that controls osteoclast formation by regulating cell motility in macrophages. International journal of molecular sciences, 23(12), 6619.
Distributed under Open Access license CC BY 4.0, without modification.

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
CBFYC-2106
Antibody Isotype
IgM, κ
Application
WB, IP, IF, ELISA, IHC-P

Basic Information

Immunogen
Amino acids 390-474 mapping at the C-terminus of human Coronin 1C.
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgM, κ
Clonality
Monoclonal Antibody
Application Notes
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
ELISA1:100-1:1,000
IHC-P1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
0.1% gelatin
Preservative
0.09% sodium azide
Concentration
0.2 mg/ml
Purity
>95% as determined by analysis by SDS-PAGE
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
coronin, actin binding protein, 1C
Entrez Gene ID
Human23603
Mouse23790
Rat501841
UniProt ID
HumanQ9ULV4
MouseQ9WUM4
RatG3V624
Function
Plays a role in directed cell migration by regulating the activation and subcellular location of RAC1 (PubMed:25074804, PubMed:25925950).

Increases the presence of activated RAC1 at the leading edge of migrating cells (PubMed:25074804, PubMed:25925950).

Required for normal organization of the cytoskeleton, including the actin cytoskeleton, microtubules and the vimentin intermediate filaments (By similarity).

Plays a role in endoplasmic reticulum-associated endosome fission: localizes to endosome membrane tubules and promotes recruitment of TMCC1, leading to recruitment of the endoplasmic reticulum to endosome tubules for fission (PubMed:30220460).

Endosome membrane fission of early and late endosomes is essential to separate regions destined for lysosomal degradation from carriers to be recycled to the plasma membrane (PubMed:30220460).

Required for normal cell proliferation, cell migration, and normal formation of lamellipodia (By similarity).

Required for normal distribution of mitochondria within cells (By similarity).

Isoform 3:
Involved in myogenic differentiation.
Biological Process
Actin filament organization Source: GO_Central
Activation of GTPase activity Source: UniProtKB
Cell migration Source: GO_Central
Endosomal transport Source: UniProtKB
Endosome fission Source: UniProtKB
Endosome membrane tubulation Source: UniProtKB
Establishment of protein localization Source: UniProtKB
Membrane fission Source: UniProtKB
Negative regulation of epithelial cell migration Source: UniProtKB
Negative regulation of focal adhesion assembly Source: UniProtKB
Negative regulation of protein kinase activity by regulation of protein phosphorylation Source: UniProtKB
Negative regulation of protein phosphorylation Source: UniProtKB
Negative regulation of substrate adhesion-dependent cell spreading Source: UniProtKB
Neural crest cell migration Source: UniProtKB
Phagocytosis Source: ProtInc
Positive regulation of lamellipodium morphogenesis Source: UniProtKB
Regulation of epithelial cell migration Source: UniProtKB
Regulation of fibroblast migration Source: UniProtKB
Regulation of focal adhesion assembly Source: UniProtKB
Regulation of protein phosphorylation Source: UniProtKB
Regulation of ruffle assembly Source: Ensembl
Regulation of substrate adhesion-dependent cell spreading Source: UniProtKB
Signal transduction Source: ProtInc
Cellular Location
Cytoskeleton; Endosome membrane; Cell membrane; Ruffle membrane; Lamellipodium; Cell cortex. All isoforms colocalize with the actin cytoskeleton in the cytosol, and especially in the cell cortex (PubMed:10828594, PubMed:19651142, PubMed:25074804). Colocalizes with F-actin at the leading edge of lamellipodia. Partially colocalizes with microtubules and vimentin intermediate filaments (PubMed:10828594, PubMed:19651142, PubMed:25074804). Localizes to endosome membrane tubules/buds (PubMed:30220460).
Isoform 3: Cytoskeleton; Sarcolemma; Cell membrane; Sarcomere; Synapse; Cell cortex. Colocalizes with the thin filaments of the sarcomere and with the postsynaptic area and the junctional sarcoplasm of motor end plates. Colocalizes with the actin cytoskeleton in the cytosol, and especially in the cell cortex.
More Infomation

Li, J., Tian, L., Jing, Z., Guo, Z., Nan, P., Liu, F., ... & Zhao, X. (2021). Cytoplasmic RAD23B interacts with CORO1C to synergistically promote colorectal cancer progression and metastasis. Cancer Letters, 516, 13-27.

Zhang, W., Song, C., & Ren, X. (2021). Circ_0003998 regulates the progression and docetaxel sensitivity of DTX-resistant non-small cell lung cancer cells by the miR-136-5p/CORO1C axis. Technology in cancer research & treatment, 20, 1533033821990040.

Wang, Z., & Jia, L. (2021). CORO1C is Associated With Poor Prognosis and Promotes Metastasis Through PI3K/AKT Pathway in Colorectal Cancer. Frontiers in molecular biosciences, 8.

Liao, M., & Peng, L. (2020). MiR-206 may suppress non-small lung cancer metastasis by targeting CORO1C. Cellular & molecular biology letters, 25(1), 1-13.

Han, S., Ding, X., Wang, S., Xu, L., Li, W., & Sun, W. (2020). miR-133a-3p regulates hepatocellular carcinoma progression through targeting CORO1C. Cancer Management and Research, 12, 8685.

Li, Q., Dai, Z., Xia, C., Jin, L., & Chen, X. (2020). Suppression of long non-coding RNA MALAT1 inhibits survival and metastasis of esophagus cancer cells by sponging miR-1-3p/CORO1C/TPM3 axis. Molecular and Cellular Biochemistry, 470(1), 165-174.

Tagliatela, A. C., Hempstead, S. C., Hibshman, P. S., Hockenberry, M. A., Brighton, H. E., Pecot, C. V., & Bear, J. E. (2020). Coronin 1C inhibits melanoma metastasis through regulation of MT1-MMP-containing extracellular vesicle secretion. Scientific reports, 10(1), 1-16.

Castagnino, A., Castro-Castro, A., Irondelle, M., Guichard, A., Lodillinsky, C., Fuhrmann, L., ... & Chavrier, P. (2018). Coronin 1C promotes triple-negative breast cancer invasiveness through regulation of MT1-MMP traffic and invadopodia function. Oncogene, 37(50), 6425-6441.

Lim, J. P., Shyamasundar, S., Gunaratne, J., Scully, O. J., Matsumoto, K., & Bay, B. H. (2017). YBX1 gene silencing inhibits migratory and invasive potential via CORO1C in breast cancer in vitro. BMC cancer, 17(1), 1-15.

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For research use only. Not intended for any clinical use.

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