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Mouse Anti-CMV gB Recombinant Antibody (CBMC-C0540) (V2LY-1206-LY385)


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Summary

Host Animal
Mouse
Specificity
CMV
Clone
CBMC-C0540
Antibody Isotype
IgG1
Application
IF, WB

Basic Information

Immunogen
CMV.
Host Species
Mouse
Specificity
CMV
Antibody Isotype
IgG1
Clonality
Monoclonal Antibody
Application Notes
ApplicationNote
WB1:100-1:1,000
IF(ICC)1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
0.1% gelatin
Preservative
<0.1% sodium azide
Concentration
0.1 mg/ml
Purity
>95% as determined by analysis by SDS-PAGE
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Cytomegalovirus Glycoprotein B
Function
Envelope glycoprotein that plays a role in host cell entry, cell to-cell virus transmission, and fusion of infected cells. May be involved in the initial attachment via binding to heparan sulfate together with the gM/gN complex that binds heparin with higher affinity. Interacts with host integrin ITGB1, PDGFRA and EGFR that likely serve as postattachment entry receptors. Participates also in the fusion of viral and cellular membranes leading to virus entry into the host cell. Membrane fusion is mediated by the fusion machinery composed at least of gB and the heterodimer gH/gL.
Biological Process
Viral entry into host cell Source: UniProtKB-UniRule
Virion attachment to host cell Source: UniProtKB-UniRule
Cellular Location
Virion membrane; Host cell membrane; Host endosome membrane; Host Golgi apparatus membrane. During virion morphogenesis, this protein probably accumulates in the endosomes and trans-Golgi where secondary envelopment occurs. It is probably transported to the cell surface from where it is endocytosed and directed to the trans-Golgi network (TGN).
Topology
Virion surface: 32-750
Helical: 751-771
Intravirion: 772-906
PTM
A proteolytic cleavage by host furin generates two subunits that remain linked by disulfide bonds.
More Infomation

Liu, Y., Heim, K. P., Che, Y., Chi, X., Qiu, X., Han, S., ... & Yang, X. (2021). Prefusion structure of human cytomegalovirus glycoprotein B and structural basis for membrane fusion. Science Advances, 7(10), eabf3178.

Zhai, R., Wang, Z., Sheng, Q., Fan, X., Kong, X., & Sun, X. (2021). Polymorphisms of the cytomegalovirus glycoprotein B genotype in patients with Posner-Schlossman syndrome. British Journal of Ophthalmology.

Jenks, J. A., Nelson, C. S., Roark, H. K., Goodwin, M. L., Pass, R. F., Bernstein, D. I., ... & Permar, S. R. (2020). Antibody binding to native cytomegalovirus glycoprotein B predicts efficacy of the gB/MF59 vaccine in humans. Science translational medicine, 12(568), eabb3611.

Puhakka, L., Pati, S., Lappalainen, M., Lönnqvist, T., Niemensivu, R., Lindahl, P., ... & Saxen, H. (2020). Viral shedding, and distribution of cytomegalovirus glycoprotein H (UL75), glycoprotein B (UL55), and glycoprotein N (UL73) genotypes in congenital cytomegalovirus infection. Journal of Clinical Virology, 125, 104287.

Reuter, N., Kropff, B., Schneiderbanger, J. K., Alt, M., Krawczyk, A., Sinzger, C., ... & Thomas, M. (2020). Cell fusion induced by a fusion-active form of human cytomegalovirus glycoprotein B (gB) is inhibited by antibodies directed at antigenic domain 5 in the ectodomain of gB. Journal of Virology, 94(18), e01276-20.

Janković, M., Ćupić, M., Knežević, A., Vujić, D., Soldatović, I., Zečević, Ž., ... & Jovanović, T. (2020). Cytomegalovirus glycoprotein B and N genotypes in pediatric recipients of the hematopoietic stem cell transplant. Virology, 548, 168-173.

Schleiss, M. R. (2018). Recombinant cytomegalovirus glycoprotein B vaccine: Rethinking the immunological basis of protection. Proceedings of the National Academy of Sciences, 115(24), 6110-6112.

Cui, X., Cao, Z., Wang, S., Lee, R. B., Wang, X., Murata, H., ... & Snapper, C. M. (2018). Novel trimeric human cytomegalovirus glycoprotein B elicits a high-titer neutralizing antibody response. Vaccine, 36(37), 5580-5590.

Ciotti, M., Cella, E., Rittà, M., Ciccozzi, M., Cavallo, R., Perno, C. F., & Costa, C. (2017). Cytomegalovirus glycoprotein B genotype distribution in Italian transplant patients. Intervirology, 60(4), 165-170.

Schleiss, M. R., Berka, U., Watson, E., Aistleithner, M., Kiefmann, B., Mangeat, B., ... & Orlinger, K. K. (2017). Additive protection against congenital cytomegalovirus conferred by combined glycoprotein B/pp65 vaccination using a lymphocytic choriomeningitis virus vector. Clinical and vaccine immunology, 24(1), e00300-16.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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