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Mouse Anti-CLDN12 Recombinant Antibody (CBXC-0450) (CBMAB-C5185-CQ)


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Summary

Host Animal
Mouse
Specificity
Human
Clone
CBXC-0450
Antibody Isotype
IgG2a, κ
Application
S-ELISA, ELISA

Basic Information

Immunogen
CLDN12 (AAH36754.1, 1 a.a.-244 a.a) Full length recombinant protein with GST tag.
Specificity
Human
Antibody Isotype
IgG2a, κ
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.2
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
claudin 12
Introduction
This gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets, and also play critical roles in maintaining cell polarity and signal transductions. This gene is expressed in the inner ear and bladder epithelium, and it is over-expressed in colorectal carcinomas. This protein and claudin 2 are critical for vitamin D-dependent Ca2+ absorption between enterocytes. Multiple alternatively spliced transcript variants encoding the same protein have been found.
Entrez Gene ID
9069
UniProt ID
P56749
Alternative Names
Claudin 12; Claudin-12;
Function
Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity.
Biological Process
Calcium-independent cell-cell adhesion via plasma membrane cell-adhesion molecules Source: UniProtKB
Maintenance of blood-brain barrier Source: ARUK-UCL
Cellular Location
Cell membrane; Tight junction
Topology
Cytoplasmic: 1-10
Helical: 11-31
Extracellular: 32-87
Helical: 88-108
Cytoplasmic: 109-135
Helical: 136-156
Extracellular: 157-174
Helical: 175-195
Cytoplasmic: 196-244
More Infomation

Rahman, A., Kobayashi, M., Sugimoto, K., Endo, Y., Kojima, M., Furukawa, S., ... & Chiba, H. (2021). Reduced Claudin-12 Expression Predicts Poor Prognosis in Cervical Cancer. International Journal of Molecular Sciences, 22(7), 3774.

Kolchakova, D., Moten, D., Batsalova, T., & Dzhambazov, B. (2021). Tight Junction Protein Claudin-12 Is Involved in Cell Migration during Metastasis. Biomolecules, 11(5), 636.

Beggs, M. R., Young, K., Pan, W., O’Neill, D. D., Saurette, M., Plain, A., ... & Alexander, R. T. (2021). Claudin-2 and claudin-12 form independent, complementary pores required to maintain calcium homeostasis. Proceedings of the National Academy of Sciences, 118(48).

Plain, A., Pan, W., O’Neill, D., Ure, M., Beggs, M. R., Farhan, M., ... & Alexander, R. T. (2020). Claudin-12 knockout mice demonstrate reduced proximal tubule calcium permeability. International journal of molecular sciences, 21(6), 2074.

Sun, L., Feng, L., & Cui, J. (2019). Increased expression of claudin‑12 promotes the metastatic phenotype of human bronchial epithelial cells and is associated with poor prognosis in lung squamous cell carcinoma. Experimental and therapeutic medicine, 17(1), 165-174.

Huang, J., Yin, P., & Zhang, L. (2019). COPII cargo claudin‐12 promotes hepatitis C virus entry. Journal of viral hepatitis, 26(2), 308-312.

Dias, M. C., Coisne, C., Baden, P., Enzmann, G., Garrett, L., Becker, L., ... & Engelhardt, B. (2019). Claudin-12 is not required for blood–brain barrier tight junction function. Fluids and Barriers of the CNS, 16(1), 1-17.

Tian, X., He, Y., Han, Z., Su, H., & Chu, C. (2019). The Cytoplasmic Expression Of CLDN12 Predicts An Unfavorable Prognosis And Promotes Proliferation And Migration Of Osteosarcoma. Cancer management and research, 11, 9339.

Du, Y., Zhou, L., Lin, Y., Yin, K., Yin, W., & Lu, J. (2019). Polymorphisms in microRNA let-7 binding sites of the HIF1AN and CLDN12 genes can predict pathologic complete response to taxane-and platinum-based neoadjuvant chemotherapy in breast cancer. Annals of translational medicine, 7(7).

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For research use only. Not intended for any clinical use.

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