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Rat Anti-CHRNA7 Recombinant Antibody (CBWJN-1068) (CBMAB-N0672-WJ)


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Summary

Host Animal
Rat
Specificity
Rat, Chicken, Human, Mouse
Clone
CBWJN-1068
Antibody Isotype
IgG1
Application
FC, IF, WB, IP, IHC-P

Basic Information

Immunogen
Bacterially expressed large cytoplasmic domain of AChRα7 of chicken.
Host Species
Rat
Specificity
Rat, Chicken, Human, Mouse
Antibody Isotype
IgG1
Clonality
Monoclonal Antibody
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
IHC-P1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Cholinergic Receptor Nicotinic Alpha 7 Subunit
Introduction
The nicotinic acetylcholine receptors (nAChRs) are members of a superfamily of ligand-gated ion channels that mediate fast signal transmission at synapses. The nAChRs are thought to be hetero-pentamers composed of homologous subunits. The proposed structure for each subunit is a conserved N-terminal extracellular domain followed by three conserved transmembrane domains, a variable cytoplasmic loop, a fourth conserved transmembrane domain, and a short C-terminal extracellular region. The protein encoded by this gene forms a homo-oligomeric channel, displays marked permeability to calcium ions and is a major component of brain nicotinic receptors that are blocked by, and highly sensitive to, alpha-bungarotoxin. Once this receptor binds acetylcholine, it undergoes an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. This gene is located in a region identified as a major susceptibility locus for juvenile myoclonic epilepsy and a chromosomal location involved in the genetic transmission of schizophrenia. An evolutionarily recent partial duplication event in this region results in a hybrid containing sequence from this gene and a novel FAM7A gene. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2012]
Entrez Gene ID
Human1139
Chicken374001
Rat25302
Monkey574230
UniProt ID
HumanP36544
ChickenP22770
RatQ05941
MonkeyQ866A2
Alternative Names
Cholinergic Receptor Nicotinic Alpha 7 Subunit; Cholinergic Receptor, Nicotinic, Alpha Polypeptide 7; Cholinergic Receptor, Nicotinic, Alpha 7 (Neuronal); Cholinergic Receptor, Nicotinic Alpha 7; NACHRA7; Neuronal Acetylcholine Receptor Protein, Alpha-7 Chain; Acetylcholine Receptor, Nicotinic, Alpha 7 (Neuronal);
Function
After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. The channel is blocked by alpha-bungarotoxin.
Biological Process
Acetylcholine receptor signaling pathway Source: ARUK-UCL
Activation of MAPK activity Source: UniProtKB
Calcium ion transport Source: UniProtKB
Cellular calcium ion homeostasis Source: UniProtKB
Chemical synaptic transmission Source: GO_Central
Cognition Source: UniProtKB
Dendrite arborization Source: ARUK-UCL
Dendritic spine organization Source: ARUK-UCL
Ion transmembrane transport Source: ParkinsonsUK-UCL
Ion transport Source: UniProtKB
Learning or memory Source: ARUK-UCL
Memory Source: ARUK-UCL
Modulation of excitatory postsynaptic potential Source: ARUK-UCL
Negative regulation of amyloid-beta formation Source: ARUK-UCL
Negative regulation of tumor necrosis factor production Source: MGI
Nervous system process Source: GO_Central
Positive regulation of amyloid-beta formation Source: ARUK-UCL
Positive regulation of angiogenesis Source: UniProtKB
Positive regulation of cell population proliferation Source: UniProtKB
Positive regulation of CoA-transferase activity Source: ARUK-UCL
Positive regulation of ERK1 and ERK2 cascade Source: ARUK-UCL
Positive regulation of excitatory postsynaptic potential Source: ARUK-UCL
Positive regulation of long-term synaptic potentiation Source: ARUK-UCL
Positive regulation of protein metabolic process Source: ARUK-UCL
Positive regulation of protein phosphorylation Source: ARUK-UCL
Regulation of amyloid fibril formation Source: ARUK-UCL
Regulation of amyloid precursor protein catabolic process Source: ARUK-UCL
Regulation of membrane potential Source: GO_Central
Regulation of neuron death Source: ARUK-UCL
Response to acetylcholine Source: ARUK-UCL
Response to amyloid-beta Source: ARUK-UCL
Response to hypoxia Source: UniProtKB
Response to nicotine Source: UniProtKB
Sensory processing Source: ARUK-UCL
Short-term memory Source: ARUK-UCL
Signal transduction Source: UniProtKB
Synapse organization Source: ARUK-UCL
Synaptic transmission, cholinergic Source: GO_Central
Cellular Location
Postsynaptic cell membrane; Cell membrane. TMEM35A/NACHO promotes its trafficking to the cell membrane (PubMed:27789755). RIC3 promotes its trafficking to the cell membrane (By similarity).
Topology
Extracellular: 23-230
Helical: 231-255
Helical: 262-280
Helical: 296-317
Cytoplasmic: 318-469
Helical: 470-490
PTM
Glycosylations at Asn-46, Asn-90 and Asn-133 are essential for TMEM35A/NACHO-mediated proper subunit assembly and trafficking to the cell membrane.
More Infomation

Wang, L., Liang, D., Xiong, X., Lin, Y., Zhu, J., Yao, Z., ... & Zhang, H. (2021). Repurposing dextromethorphan and metformin for treating nicotine-induced cancer by directly targeting CHRNA7 to inhibit JAK2/STAT3/SOX2 signaling. Oncogene, 40(11), 1974-1987.

Briggs, F. B. (2021). Nicotinic acetylcholine receptors α7 and α9 modifies tobacco smoke risk for multiple sclerosis. Multiple Sclerosis Journal, 27(8), 1166-1174.

Özaltun, M. F., Geyik, S., & Yılmaz, Ş. G. (2021). Screening for Copy Number Variations of the 15q13. 3 Hotspot in CHRNA7 Gene and Expression in Patients with Migraines. Current issues in molecular biology, 43(2), 1090-1113.

Santos, J. R., Tomaz, P. R. X., Scholz, J. R., Gaya, P. V., Abe, T. O., Krieger, J. E., ... & Santos, P. C. J. D. L. (2020). Profile of the Nicotinic Cholinergic Receptor Alpha 7 Subunit Gene Expression is Associated with Response to Varenicline Treatment. Genes, 11(7), 746.

Chan, T., Williams, E., Cohen, O., Eliceiri, B. P., Baird, A., & Costantini, T. W. (2019). CHRFAM7A alters binding to the neuronal alpha-7 nicotinic acetylcholine receptor. Neuroscience letters, 690, 126-131.

Calabrò, M., Mandelli, L., Crisafulli, C., Lee, S. J., Jun, T. Y., Wang, S. M., ... & Serretti, A. (2019). Genes involved in neurodevelopment, neuroplasticity and major depression: no association for CACNA1C, CHRNA7 and MAPK1. Clinical Psychopharmacology and Neuroscience, 17(3), 364.

De Jaco, A., Bernardini, L., Rosati, J., & Maria Tata, A. (2017). Alpha-7 nicotinic receptors in nervous system disorders: from function to therapeutic perspectives. Central Nervous System Agents in Medicinal Chemistry (Formerly Current Medicinal Chemistry-Central Nervous System Agents), 17(2), 100-108.

Yadav, R., Deepshikha, D., & Srivastava, P. (2017). Homology modeling and protein interaction map of CHRNA7 neurogenesis protein. Annals of neurosciences, 24(3), 173-179.

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For research use only. Not intended for any clinical use.

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