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Mouse Anti-CHMP6 Recombinant Antibody (CBXC-1748) (CBMAB-C5596-CQ)


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Published Data
CBMAB-C5596-CQ-1
Mouse Anti-CHMP6 Recombinant Antibody (CBXC-1748) (CBMAB-C5596-CQ) in IF
i3Neurons expressing the guides indicated were fixed and labeled for CHMP6, then visualized by confocal immunofluorescence microscopy. The number of CHMP6 puncta per cell was quantified in 3 biological repeats (≥20 cells per experimental condition in each repeat). Bars indicate mean ± SEM, p values calculated by one-way ANOVA with Tukey's correction for multiple testing. Micrograph scale bar = 10 μm. ...View More
Rodger, C., Flex, E., Allison, R. J., Sanchis-Juan, A., Hasenahuer, M. A., Cecchetti, S., ... & Reid, E. (2020). De novo VPS4A mutations cause multisystem disease with abnormal neurodevelopment. The American Journal of Human Genetics, 107(6), 1129-1148.
Distributed under Open Access license CC BY 4.0, without modification.

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
CBXC-1748
Antibody Isotype
IgG1, κ
Application
WB, IP, IF, ELISA

Basic Information

Immunogen
Amino acids 1-201 representing full length CHMP6 of human.
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG1, κ
Clonality
Monoclonal Antibody
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
ELISA1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Charged Multivesicular Body Protein 6
Introduction
This gene encodes a member of the chromatin-modifying protein/charged multivesicular body protein family. Proteins in this family are part of the ESCRT-III (endosomal sorting complex required for transport III) which degrades surface receptors, and in biosynthesis of endosomes.
Entrez Gene ID
79643
UniProt ID
Q96FZ7
Alternative Names
Charged Multivesicular Body Protein 6; Vacuolar Protein Sorting-Associated Protein 20; Chromatin-Modifying Protein 6; HVps20; VPS20; Chromatin Modifying Protein 6;
Function
Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and the budding of enveloped viruses (HIV-1 and other lentiviruses). ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4. In the ESCRT-III complex, it probably serves as an acceptor for the ESCRT-II complex on endosomal membranes.
Biological Process
Endosomal transport Source: Reactome
ESCRT III complex assembly Source: ParkinsonsUK-UCL
Late endosome to vacuole transport via multivesicular body sorting pathway Source: GO_Central
Macroautophagy Source: ParkinsonsUK-UCL
Midbody abscission Source: UniProtKB
Mitotic metaphase plate congression Source: UniProtKB
Mitotic nuclear envelope reassembly Source: Reactome
Multivesicular body assembly Source: ParkinsonsUK-UCL
Negative regulation of epidermal growth factor-activated receptor activity Source: UniProtKB
Nucleus organization Source: UniProtKB
Protein transport Source: UniProtKB-KW
Regulation of protein catabolic process Source: UniProtKB
Vesicle budding from membrane Source: GO_Central
Viral budding via host ESCRT complex Source: UniProtKB
Viral life cycle Source: Reactome
Cellular Location
Endosome membrane; Late endosome membrane; Endomembrane system; Membrane. Localizes to endosomal membranes.
PTM
ISGylated in a CHMP5-dependent manner. Isgylation weakens its interaction with VPS4A.
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For research use only. Not intended for any clinical use.

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