Search :
Sign in or Register  
Welcome Sign in or Don't have an account?Register

Mouse Anti-CFL1 (Phospho-Ser3) Recombinant Antibody (CBFYC-0375) (CBMAB-C0415-FY)


See all CFL1 antibodies
Online Inquiry
Published Data
CBMAB-C0415-FY-1
Mouse Anti-CFL1 Recombinant Antibody (CBFYC-0375) (CBMAB-C0415-FY) in WB
Representative immunoblot of mediators of neuronal survival and synaptic dynamics/plasticity, (A) t-PI3K, (B) p-PI3K, (C) t-Akt, (D) p-Akt, (E) t-GSK-3β, (F) GSK-3β, (G) t-PAK, (H) p-PAK, (I) t-LIMK-1, (J) p-LIMK-1, (K) t-cofilin-1, (L) p-cofilin-1, and (M) β-actin, as a loading control, in hippocampal synaptosomes. ...View More
Ansari, M. A., Al-Jarallah, A., & Babiker, F. A. (2023). Impaired insulin signaling alters mediators of hippocampal synaptic dynamics/plasticity: a possible mechanism of hyperglycemia-induced cognitive impairment. Cells, 12(13), 1728.
Distributed under Open Access license CC BY 4.0, without modification.
CBMAB-C0415-FY-2
Mouse Anti-CFL1 Recombinant Antibody (CBFYC-0375) (CBMAB-C0415-FY) in WB
Representative images of Western blots of the synaptosomal extracts of 2R control neurons and 66R neurons are shown. ...View More
Huber, N., Hoffmann, D., Giniatullina, R., Rostalski, H., Leskelä, S., Takalo, M., ... & Haapasalo, A. (2022). C9orf72 hexanucleotide repeat expansion leads to altered neuronal and dendritic spine morphology and synaptic dysfunction. Neurobiology of Disease, 162, 105584.
Distributed under Open Access license CC BY 4.0, without modification.
CBMAB-C0415-FY-3
Mouse Anti-CFL1 Recombinant Antibody (CBFYC-0375) (CBMAB-C0415-FY) in WB
The expression of the indicated genes in the cells expressing miR-335 inhibitor or the combination was determined by Western blot and RT-PCR, separately. *P < 0.05 vs. control, #P < 0.05 vs. miR-335 inhibitor. Data gained in three independent experiments. The results are presented as the mean ± standard deviation. ...View More
Cheng, Y., & Shen, P. (2020). miR-335 acts as a tumor suppressor and enhances ionizing radiation-induced tumor regression by targeting ROCK1. Frontiers in oncology, 10, 278.
Distributed under Open Access license CC BY 4.0, without modification.
CBMAB-C0415-FY-4
Mouse Anti-CFL1 Recombinant Antibody (CBFYC-0375) (CBMAB-C0415-FY) in WB
ata are presented as total pixels (green) per soma (Dapi, blue). GTP-Rac1 (E) and GTP-Cdc42 (G) treated cell lysate were probed for P-Cav-1, T-Cav-1, and P-Cofilin (F for GTP-Rac; H for GTP-Cdc42). Data are expressed as mean ± S.E (n = 3, *p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, *⁣*⁣**p < 0.0001, One-way ANOVA). ...View More
Wang, S., Zhang, Z., Almenar-Queralt, A., Leem, J., DerMardirossian, C., Roth, D. M., ... & Head, B. P. (2019). Caveolin-1 phosphorylation is essential for axonal growth of human neurons derived from iPSCs. Frontiers in Cellular Neuroscience, 13, 324.
Distributed under Open Access license CC BY 4.0, without modification.
CBMAB-C0415-FY-5
Mouse Anti-CFL1 Recombinant Antibody (CBFYC-0375) (CBMAB-C0415-FY) in WB
The overexpression of DHCR24 significantly increased NLG1 levels upon basal growth conditions in primary neuron-BV2 cell co-cultures. A similar trend towards increase was also observed in co-cultures overexpressing DHCR24 upon LPS and IFN-γ treatment. The assessment of phosphorylation status of cofilin-1 at Ser 3 did not show statically significant changes between DHCR24-overexpressing or control co-cultures after vehicle or LPS and IFN-γ treatments. The induction of neuroinflammation per se significantly reduced the phosphorylation cofilin-1 at Ser 3. ...View More
Martiskainen, H., Paldanius, K. M., Natunen, T., Takalo, M., Marttinen, M., Leskelä, S., ... & Hiltunen, M. (2017). DHCR24 exerts neuroprotection upon inflammation-induced neuronal death. Journal of neuroinflammation, 14, 1-16.
Distributed under Open Access license CC BY 4.0, without modification.

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat, Cattle, Pig
Clone
CBFYC-0375
Antibody Isotype
IgM, κ
Application
IF, IHC-P, WB, ELISA, IP

Basic Information

Immunogen
Amino acid sequence containing phosphorylated Ser 3 of Cofilin 1 of human.
Host Species
Mouse
Specificity
Human, Mouse, Rat, Cattle, Pig
Antibody Isotype
IgM, κ
Clonality
Monoclonal Antibody
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
IHC-P1:50-1:500
ELISA1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
AA 1-166

Target

Full Name
Cofilin 1
Introduction
The protein encoded by this gene can polymerize and depolymerize F-actin and G-actin in a pH-dependent manner. Increased phosphorylation of this protein by LIM kinase aids in Rho-induced reorganization of the actin cytoskeleton. Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. It is involved in the translocation of actin-cofilin complex from cytoplasm to nucleus.
Entrez Gene ID
1072
UniProt ID
P23528
Alternative Names
Cofilin 1; Cofilin, Non-Muscle Isoform; Cofilin 1 (Non-Muscle); 18 KDa Phosphoprotein; P18; CFL
Function
Binds to F-actin and exhibits pH-sensitive F-actin depolymerizing activity (PubMed:11812157).
In conjunction with the subcortical maternal complex (SCMC), plays an essential role for zygotes to progress beyond the first embryonic cell divisions via regulation of actin dynamics (PubMed:15580268).
Required for the centralization of the mitotic spindle and symmetric division of zygotes (By similarity).
Plays a role in the regulation of cell morphology and cytoskeletal organization in epithelial cells (PubMed:21834987).
Required for the up-regulation of atypical chemokine receptor ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation (PubMed:23633677).
Required for neural tube morphogenesis and neural crest cell migration (By similarity).
Biological Process
Actin cytoskeleton organization Source: ProtInc
Actin filament depolymerization Source: UniProtKB
Actin filament fragmentation Source: GO_Central
Actin filament severing Source: GO_Central
Cell motility Source: GO_Central
Cytoskeleton organization Source: UniProtKB
Establishment of spindle localization Source: UniProtKB
Interleukin-12-mediated signaling pathway Source: Reactome
Negative regulation of apoptotic process Source: UniProtKB
Positive regulation by host of viral process Source: AgBase
Positive regulation of embryonic development Source: UniProtKB
Regulation of cell morphogenesis Source: UniProtKB
Regulation of dendritic spine morphogenesis Source: ParkinsonsUK-UCL
Response to virus Source: UniProtKB
Rho protein signal transduction Source: ProtInc
Viral process Source: UniProtKB-KW
Cellular Location
Ruffle membrane; Lamellipodium membrane; Cytoskeleton; Nucleus matrix; Lamellipodium. Colocalizes with the actin cytoskeleton in membrane ruffles and lamellipodia. Detected at the cleavage furrow and contractile ring during cytokinesis. Almost completely in nucleus in cells exposed to heat shock or 10% dimethyl sulfoxide.
PTM
Inactivated by phosphorylation on Ser-3. Phosphorylated on Ser-3 in resting cells (By similarity). Dephosphorylated by PDXP/chronophin; this restores its activity in promoting actin filament depolymerization. The phosphorylation of Ser-24 may prevent recognition of the nuclear localization signal (By similarity). Phosphorylated via a ARRB1-RAC1-LIMK1-PAK1 cascade upon active ligand stimulation of atypical chemokine receptor ACKR2.
More Infomation

Li, Y., Gong, Y., Chen, Y., Qu, B., & Zhang, S. (2022). Identification and functional characterization of Cofilin-1 as a new member of antimicrobial protein. Developmental & Comparative Immunology, 127, 104281.

Yao, B., Li, Y., Chen, T., Niu, Y., Wang, Y., Yang, Y., ... & Tu, K. (2021). Hypoxia‐induced cofilin 1 promotes hepatocellular carcinoma progression by regulating the PLD1/AKT pathway. Clinical and translational medicine, 11(3), e366.

Sousa-Squiavinato, A. C. M., Vasconcelos, R. I., Gehren, A. S., Fernandes, P. V., de Oliveira, I. M., Boroni, M., & Morgado-Díaz, J. A. (2021). Cofilin-1, LIMK1 and SSH1 are differentially expressed in locally advanced colorectal cancer and according to consensus molecular subtypes. Cancer Cell International, 21(1), 1-15.

Werle, S. D., Schwab, J. D., Tatura, M., Kirchhoff, S., Szekely, R., Diels, R., ... & Kestler, H. A. (2021). Unraveling the molecular tumor-promoting regulation of cofilin-1 in pancreatic cancer. Cancers, 13(4), 725.

Cho, H. J., Baek, G. O., Yoon, M. G., Ahn, H. R., Son, J. A., Kim, S. S., ... & Eun, J. W. (2021). Overexpressed proteins in HCC Cell-derived exosomes, CCT8, and Cofilin-1 are potential biomarkers for patients with HCC. Diagnostics, 11(7), 1221.

Zhang, L., Chai, Z., Kong, S., Feng, J., Wu, M., Tan, J., ... & Xu, H. (2021). Nujiangexanthone A Inhibits Hepatocellular Carcinoma Metastasis via Down Regulation of Cofilin 1. Frontiers in Cell and Developmental Biology, 9, 476.

Medina, C., de la Fuente, V., tom Dieck, S., Nassim-Assir, B., Dalmay, T., Bartnik, I., ... & Romano, A. (2020). LIMK, Cofilin 1 and actin dynamics involvement in fear memory processing. Neurobiology of Learning and Memory, 173, 107275.

Rangel, M. P., Antonangelo, L., Acencio, M. M. P., Faria, C. S., de Sá, V. K., Leão, P. S., ... & Capelozzi, V. L. (2018). Detection of sputum cofilin-1 as indicator of malignancy. Brazilian Journal of Medical and Biological Research, 51.

Shishkin, S., Eremina, L., Pashintseva, N., Kovalev, L., & Kovaleva, M. (2017). Cofilin-1 and other ADF/cofilin superfamily members in human malignant cells. International journal of molecular sciences, 18(1), 10.

Wang, H., Tao, L., Jin, F., Gu, H., Dai, X., Ni, T., ... & Liu, Y. (2017). Cofilin 1 induces the epithelial-mesenchymal transition of gastric cancer cells by promoting cytoskeletal rearrangement. Oncotarget, 8(24), 39131.

Ask a question We look forward to hearing from you.
0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Loading...
Have you used Mouse Anti-CFL1 (Phospho-Ser3) Recombinant Antibody (CBFYC-0375)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon $30 eGift Card
Submit a review
Loading...
For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Learn more

Documents

Request bulk or custom quoteOnline Inquiry
Second antibody and isotype control

Contact us

  • Tel: (USA)
  • (UK)
  • Fax:
  • Email:

Submit A Review

Online Inquiry