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Mouse Anti-CEACAM7 Recombinant Antibody (BAC2) (CBMAB-C3522WJ)


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Published Data
CBMAB-C3522WJ-1
Mouse Anti-CEACAM7 Recombinant Antibody (BAC2) (CBMAB-C3522WJ) in WB
C2BBe1 cells were cultured on cell culture plates or Transwell filters for 7 or 21 days as indicated. Cell lysates were analyzed by Western blotting for the expression of CEACAM1, CEACAM5, CEACAM6, CEACAM7, and actin. As positive controls, cells were treated for 48 h with 100 ng/ml IFN-γ (IFNg) in order to induce enhanced CEACAM expression. Note the reduced CEACAM1 and CEACAM6 expression and the abolished CEACAM5 expression in well-differentiated cells. Panels are representative of at least two independent experiments. Untr., untreated. ...View More
Klaile, E., Müller, M. M., Schäfer, M. R., Clauder, A. K., Feer, S., Heyl, K. A., ... & Slevogt, H. (2017). Binding of Candida albicans to human CEACAM1 and CEACAM6 modulates the inflammatory response of intestinal epithelial cells. MBio, 8(2), 10-1128.
Distributed under Open Access license CC BY 4.0, without modification.
CBMAB-C3522WJ-2
Mouse Anti-CEACAM7 Recombinant Antibody (BAC2) (CBMAB-C3522WJ) in FC
C2BBe1 cells were cultured on cell culture plates for 14 days. Cells were analyzed by flow cytometry for the expression of CEACAM1, CEACAM5, CEACAM6, and CEACAM7. As positive controls, cells were treated for 48 h with 100 ng/ml interferon gamma or with 1 mM H2O2 (two stimulations at 0 h and at 24 h) to induce enhanced CEACAM expression. ...View More
Klaile, E., Müller, M. M., Schäfer, M. R., Clauder, A. K., Feer, S., Heyl, K. A., ... & Slevogt, H. (2017). Binding of Candida albicans to human CEACAM1 and CEACAM6 modulates the inflammatory response of intestinal epithelial cells. MBio, 8(2), 10-1128.
Distributed under Open Access license CC BY 4.0, without modification.

Summary

Host Animal
Mouse
Specificity
Human
Clone
BAC2
Antibody Isotype
IgG1, κ
Application
IF, FC

Basic Information

Immunogen
CEACAM7 of human.
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG1, κ
Clonality
Monoclonal Antibody
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
IF(ICC)1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Carcinoembryonic Antigen Related Cell Adhesion Molecule 7
Introduction
This gene encodes a cell surface glycoprotein and member of the carcinoembryonic antigen (CEA) family of proteins. Expression of this gene may be downregulated in colon and rectal cancer. Additionally, lower expression levels of this gene may be predictive of rectal cancer recurrence. This gene is present in a CEA family gene cluster on chromosome 19. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2015]
Entrez Gene ID
1087
UniProt ID
Q14002
Alternative Names
Carcinoembryonic Antigen Related Cell Adhesion Molecule 7; Carcinoembryonic Antigen-Related Cell Adhesion Molecule 7; Carcinoembryonic Antigen Gene Family Member 2; Carcinoembryonic Antigen CGM2; CGM2;
Cellular Location
Cell membrane; Apical cell membrane. Localized to the apical glycocalyx surface.
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For research use only. Not intended for any clinical use.

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