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Mouse Anti-CDK4 Recombinant Antibody (CBFYC-1598) (CBMAB-C1657-FY)


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Published Data
CBMAB-C1657-FY-1
Mouse Anti-CDK4 Recombinant Antibody (CBFYC-1598) (CBMAB-C1657-FY) in WB
Western blot analysis of the expression of the cyclin-dependent kinases Cdk4 and Cdk6 and Cyclin D1 in PH2 cells and PN24 cells. Cdk4, Cdk6, and Cyclin D1 were increased in PN24 cells. ...View More
Agborbesong, E., Zhou, J. X., Zhang, H., Li, L. X., Harris, P. C., Calvet, J. P., & Li, X. (2024). Overexpression of SMYD3 Promotes Autosomal Dominant Polycystic Kidney Disease by Mediating Cell Proliferation and Genome Instability. Biomedicines, 12(3), 603.
Distributed under Open Access license CC BY 4.0, without modification.
CBMAB-C1657-FY-2
Mouse Anti-CDK4 Recombinant Antibody (CBFYC-1598) (CBMAB-C1657-FY) in WB
U87MG cells were transfected with either CaMKIIγ siRNA or CDK4 siRNA. Knockdown of CDK4 genes was confirmed by Western blot analysis. ...View More
Han, J. M., & Jung, H. J. (2022). Synergistic anticancer effect of a combination of berbamine and arcyriaflavin A against glioblastoma stem-like cells. Molecules, 27(22), 7968.
Distributed under Open Access license CC BY 4.0, without modification.
CBMAB-C1657-FY-3
Mouse Anti-CDK4 Recombinant Antibody (CBFYC-1598) (CBMAB-C1657-FY) in IF
CDK4 foci. Blue (DAPI) = nucleus, green (GFP) = leukemic cells, yellow (AF555) = CDK4. ...View More
Vorwerk, J., Sun, K., Frank, D., Neumann, F., Hüve, J., Budde, P. M., ... & Khandanpour, C. (2022). Presence of the GFI1-36N single nucleotide polymorphism enhances the response of MLL-AF9 leukemic cells to CDK4/6 inhibition. Frontiers in Oncology, 12, 903691.
Distributed under Open Access license CC BY 4.0, without modification.
CBMAB-C1657-FY-4
Mouse Anti-CDK4 Recombinant Antibody (CBFYC-1598) (CBMAB-C1657-FY) in WB
Western blotting analysis of cellular G0/G1 phase regulators CDK4, CDK2 and cyclin D1 under HG and arctiin treatment for 48 h. ...View More
Zhou, M., Li, G., Zhu, L., Zhou, H., & Lu, L. (2020). Arctiin attenuates high glucose-induced human retinal capillary endothelial cell proliferation by regulating ROCK1/PTEN/PI3K/Akt/VEGF pathway in vitro. Journal of Cellular and Molecular Medicine, 24(10), 5695-5706.
Distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
CBMAB-C1657-FY-5
Mouse Anti-CDK4 Recombinant Antibody (CBFYC-1598) (CBMAB-C1657-FY) in WB
After 24 h, total cell lysates were subjected to detect expression of cell cycle arrest-related proteins in HepG2 cells. Data values were expressed as mean ± SD of triplicate determinations. Significant differences were compared with control at *p < 0.05, **p < 0.01, and ***p < 0.001 using one-way ANOVA. ...View More
Cho, H. D., Min, H. J., Won, Y. S., Ahn, H. Y., Cho, Y. S., & Seo, K. I. (2019). Solid state fermentation process with Aspergillus kawachii enhances the cancer-suppressive potential of silkworm larva in hepatocellular carcinoma cells. BMC complementary and alternative medicine, 19, 1-15.
Distributed under Open Access license CC BY 4.0, without modification.

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBFYC-1598
Antibody Isotype
IgG1, κ
Application
IF, IHC-P, IP, WB

Basic Information

Immunogen
Full length recombinant human Cdk4.
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG1, κ
Clonality
Monoclonal Antibody
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
IHC-P1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Cyclin Dependent Kinase 4
Introduction
CDK4 exists, in part, as a multi-protein complex with a D-type cyclin, proliferating cell nuclear antigen and a protein, p21 (refs 7–9)
Entrez Gene ID
1019
UniProt ID
P11802
Alternative Names
Cyclin Dependent Kinase 4; Cell Division Protein Kinase 4; EC 2.7.11.22; PSK-J3; Cyclin-Dependent Kinase 4; EC 2.7.11; CMM3
Function
Ser/Thr-kinase component of cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G1/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complexes and the subsequent transcription of E2F target genes which are responsible for the progression through the G1 phase. Hypophosphorylates RB1 in early G1 phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also phosphorylates SMAD3 in a cell-cycle-dependent manner and represses its transcriptional activity. Component of the ternary complex, cyclin D/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex.
Biological Process
Adipose tissue development Source: Ensembl
Animal organ regeneration Source: Ensembl
Cell division Source: UniProtKB-KW
Cellular response to insulin stimulus Source: Ensembl
Cellular response to interleukin-4 Source: Ensembl
Cellular response to ionomycin Source: Ensembl
Cellular response to lipopolysaccharide Source: Ensembl
Cellular response to phorbol 13-acetate 12-myristate Source: Ensembl
Circadian rhythm Source: Ensembl
G1/S transition of mitotic cell cycle Source: BHF-UCL
Granulocyte differentiation Source: Reactome
Lens development in camera-type eye Source: Ensembl
Negative regulation of cell cycle arrest Source: UniProtKB
Negative regulation of G1/S transition of mitotic cell cycle Source: Reactome
Positive regulation of apoptotic process Source: Ensembl
Positive regulation of cell cycle Source: Reactome
Positive regulation of cell population proliferation Source: BHF-UCL
Positive regulation of cell size Source: Ensembl
Positive regulation of fibroblast proliferation Source: BHF-UCL
Positive regulation of G2/M transition of mitotic cell cycle Source: UniProtKB
Positive regulation of translation Source: Ensembl
Protein phosphorylation Source: BHF-UCL
Regulation of cell cycle Source: GO_Central
Regulation of gene expression Source: BHF-UCL
Regulation of insulin receptor signaling pathway Source: Ensembl
Regulation of lipid biosynthetic process Source: Ensembl
Regulation of lipid catabolic process Source: Ensembl
Regulation of multicellular organism growth Source: Ensembl
Regulation of transcription initiation from RNA polymerase II promoter Source: Reactome
Response to drug Source: UniProtKB
Response to hyperoxia Source: Ensembl
Response to lead ion Source: Ensembl
Response to testosterone Source: Ensembl
Response to toxic substance Source: Ensembl
Signal transduction Source: Ensembl
Cellular Location
Nucleus; Nucleus membrane; Cytoplasm. Cytoplasmic when non-complexed. Forms a cyclin D-CDK4 complex in the cytoplasm as cells progress through G1 phase. The complex accumulates on the nuclear membrane and enters the nucleus on transition from G1 to S phase. Also present in nucleoli and heterochromatin lumps. Colocalizes with RB1 after release into the nucleus.
Involvement in disease
Melanoma, cutaneous malignant 3 (CMM3): A malignant neoplasm of melanocytes, arising de novo or from a pre-existing benign nevus, which occurs most often in the skin but also may involve other sites.
PTM
Phosphorylation at Thr-172 is required for enzymatic activity. Phosphorylated, in vitro, at this site by CCNH-CDK7, but, in vivo, appears to be phosphorylated by a proline-directed kinase. In the cyclin D-CDK4-CDKN1B complex, this phosphorylation and consequent CDK4 enzyme activity, is dependent on the tyrosine phosphorylation state of CDKN1B. Thus, in proliferating cells, CDK4 within the complex is phosphorylated on Thr-172 in the T-loop. In resting cells, phosphorylation on Thr-172 is prevented by the non-tyrosine-phosphorylated form of CDKN1B.
More Infomation

Finn, R. S., Liu, Y., Zhu, Z., Martin, M., Rugo, H. S., Diéras, V., ... & Slamon, D. J. (2020). Biomarker analyses of response to cyclin-dependent kinase 4/6 inhibition and endocrine therapy in women with treatment-naive metastatic breast cancer. Clinical Cancer Research, 26(1), 110-121.

Wang, P. F., Qiu, H. Y., He, Y., & Zhu, H. L. (2020). Cyclin-dependent kinase 4/6 inhibitors for cancer therapy: a patent review (2015–2019). Expert Opinion on Therapeutic Patents, 30(10), 795-805.

Spring, L. M., Wander, S. A., Andre, F., Moy, B., Turner, N. C., & Bardia, A. (2020). Cyclin-dependent kinase 4 and 6 inhibitors for hormone receptor-positive breast cancer: past, present, and future. The Lancet, 395(10226), 817-827.

Zheng, J., Wu, J., Wang, C., Zhuang, S., Chen, J., & Ye, F. (2020). Combination cyclin-dependent kinase 4/6 inhibitors and endocrine therapy versus endocrine monotherapy for hormonal receptor-positive, human epidermal growth factor receptor 2-negative advanced breast cancer: A systematic review and meta-analysis. PloS one, 15(6), e0233571.

Guiley, K. Z., Stevenson, J. W., Lou, K., Barkovich, K. J., Kumarasamy, V., Wijeratne, T. U., ... & Rubin, S. M. (2019). p27 allosterically activates cyclin-dependent kinase 4 and antagonizes palbociclib inhibition. Science, 366(6471).

Thill, M., & Schmidt, M. (2018). Management of adverse events during cyclin-dependent kinase 4/6 (CDK4/6) inhibitor-based treatment in breast cancer. Therapeutic advances in medical oncology, 10, 1758835918793326.

Zhou, Y., Shen, J. K., Yu, Z., Hornicek, F. J., Kan, Q., & Duan, Z. (2018). Expression and therapeutic implications of cyclin-dependent kinase 4 (CDK4) in osteosarcoma. Biochimica et Biophysica Acta (BBA)-Molecular Basis of Disease, 1864(5), 1573-1582.

Battisti, N. M. L., De Glas, N., Sedrak, M. S., Loh, K. P., Liposits, G., Soto-Perez-de-Celis, E., ... & Ring, A. (2018). Use of cyclin-dependent kinase 4/6 (CDK4/6) inhibitors in older patients with ER-positive HER2-negative breast cancer: Young International Society of Geriatric Oncology review paper. Therapeutic advances in medical oncology, 10, 1758835918809610.

Tripathy, D., Bardia, A., & Sellers, W. R. (2017). Ribociclib (LEE011): mechanism of action and clinical impact of this selective cyclin-dependent kinase 4/6 inhibitor in various solid tumors. Clinical Cancer Research, 23(13), 3251-3262.

Kwapisz, D. (2017). Cyclin-dependent kinase 4/6 inhibitors in breast cancer: palbociclib, ribociclib, and abemaciclib. Breast cancer research and treatment, 166(1), 41-54.

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