Mouse Anti-CDH4 Recombinant Antibody (CBFYC-1564) (CBMAB-C1623-FY)

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Datasheet

Summary

Host Animal

Mouse

Specificity

Human, Mouse, Rat, Cattke, Dog

Clone

CBFYC-1564

Antibody Isotype

IgG2a, κ

Application

WB, ELISA, IF, IP, IHC-P

Basic Information

Immunogen

Amino acids 190-223 at the N-terminus of R-cadherin of human.

Host Species

Mouse

Specificity

Human, Mouse, Rat, Cattke, Dog

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
IHC-P	1:50-1:500
ELISA	1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

Epitope

AA 170-428

Target

Full Name

Cadherin 4

Introduction

CDH4 gene, a member of the cadherin family encoding for R-cadherin, contains a CpG island located at the 5′ of the first exon, which functions as a promoter element and is frequently affected by methylation in human cancer.

Entrez Gene ID

1002

UniProt ID

P55283

Alternative Names

Cadherin 4; R-Cadherin; Cadherin 4, Type 1, R-Cadherin (Retinal); Retinal Cadherin; R-CAD; Cadherin 4, Type 1, Preproprotein; Cadherin-4; CAD4; RCAD

Function

Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. May play an important role in retinal development.

Biological Process

Adherens junction organization Source: Reactome
Axon guidance Source: Ensembl
Cell adhesion Source: ProtInc
Cell-cell adhesion via plasma-membrane adhesion molecules Source: GO_Central
Heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules Source: Ensembl
Homophilic cell adhesion via plasma membrane adhesion molecules Source: GO_Central
Positive regulation of axon extension Source: Ensembl

Cellular Location

Cell membrane

Topology

Extracellular: 170-734
Helical: 735-756
Cytoplasmic: 757-916

More Infomation

References

Wu, L., Ding, Y., Tong, H., Zhuang, X., Cai, J., Si, Y., ... & Shen, M. (2021). Long noncoding RNA FER1L4 promotes the malignant processes of papillary thyroid cancer by targeting the miR-612/Cadherin 4 axis. Cancer cell international, 21(1), 1-16.

Wu, L., Ding, Y., Zhuang, X., Cai, J., Tong, H., Si, Y., ... & Shen, M. (2021). Long Noncoding RNA FER1L4 Promotes Papillary Thyroid Cancer Progression by Targeting miR-612/CDH4 Axis.

Zhang, N., Li, L., Long, Z., Du, J., Li, S., Yin, H., ... & Wang, F. (2021). Are dietary factors involved in the association of CDH4 methylation and breast cancer risk?. British Journal of Nutrition, 1-10.

Zhou, X., Huang, H., Cui, W., Wang, Y., Luo, W., Matskova, L., & Zhou, X. (2020). Expression and prognostic significance of cadherin 4 (CDH4) in renal cell carcinoma. Medical Science Monitor: International Medical Journal of Experimental and Clinical Research, 26, e922836-1.

Rangel Olguin, A. G. (2020). The role of Cadherin 4 in the assembly of retinal circuits.

Ceresa, D., Alessandrini, F., Bosio, L., Marubbi, D., Reverberi, D., Malatesta, P., & Appolloni, I. (2019). Cdh4 down-regulation impairs in vivo infiltration and malignancy in patients derived glioblastoma cells. International journal of molecular sciences, 20(16), 4028.

Aburegeba, Z. (2018). Characterization of suppressors of fat-like cadherin CDH-4 in context of axon guidance during embryonic development (Doctoral dissertation, Science: Biological Sciences Department).

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Associated Products

Related Products

Mouse Anti-CDH4 Recombinant Antibody (2E2)

Rabbit Anti-CDH4 Recombinant Antibody (EG594)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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Documents

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SDS
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Second antibody and isotype control

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