Mouse Anti-CDC34 Recombinant Antibody (CBFYC-1517) (CBMAB-C1576-FY)

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Datasheet

Summary

Host Animal

Mouse

Specificity

Human, Mouse, Rat

Clone

CBFYC-1517

Antibody Isotype

IgG2a

Application

IP, WB

Basic Information

Immunogen

E. coli-derived recombinant protein of amino acids 1-236 of human CDC34.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG2a

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS

Preservative

0.09% sodium azide

Concentration

1 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name

Cell Division Cycle 34

Introduction

CDC34 (Cell Division Cycle 34) is a Protein Coding gene. Among its related pathways are Metabolism of proteins and CLEC7A (Dectin-1) signaling. Gene Ontology (GO) annotations related to this gene include ligase activity and ubiquitin protein ligase binding. An important paralog of this gene is UBE2R2.

Entrez Gene ID

997

UniProt ID

P49427

Alternative Names

Cell Division Cycle 34; (E3-Independent) E2 Ubiquitin-Conjugating Enzyme R1; Ubiquitin-Conjugating Enzyme E2-CDC34; E2 Ubiquitin-Conjugating Enzyme R1; Ubiquitin-Protein Ligase R1; UBE2R1; UBCH3; Ubiquitin-Conjugating Enzyme E2-32 KDA Complementing; Ubiquitin-Conjugating Enzyme E2-32 KDa Complementing; Ubiquitin Conjugating Enzyme E2 R1

Function

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-48'-linked polyubiquitination (PubMed:22496338).
Cooperates with the E2 UBCH5C and the SCF(FBXW11) E3 ligase complex for the polyubiquitination of NFKBIA leading to its subsequent proteasomal degradation. Performs ubiquitin chain elongation building ubiquitin chains from the UBE2D3-primed NFKBIA-linked ubiquitin. UBE2D3 acts as an initiator E2, priming the phosphorylated NFKBIA target at positions 'Lys-21' and/or 'Lys-22' with a monoubiquitin. Cooperates with the SCF(SKP2) E3 ligase complex to regulate cell proliferation through ubiquitination and degradation of MYBL2 and KIP1. Involved in ubiquitin conjugation and degradation of CREM isoform ICERIIgamma and ATF15 resulting in abrogation of ICERIIgamma- and ATF5-mediated repression of cAMP-induced transcription during both meiotic and mitotic cell cycles. Involved in the regulation of the cell cycle G2/M phase through its targeting of the WEE1 kinase for ubiquitination and degradation. Also involved in the degradation of beta-catenin. Is target of human herpes virus 1 protein ICP0, leading to ICP0-dependent dynamic interaction with proteasomes (PubMed:10329681, PubMed:10373550, PubMed:10871850, PubMed:11675391, PubMed:12037680, PubMed:15652359, PubMed:17461777, PubMed:17698585, PubMed:19112177, PubMed:19126550, PubMed:19945379, PubMed:20061386, PubMed:20347421).

Biological Process

Cellular protein modification process Source: UniProtKB
Cellular response to interferon-beta Source: UniProtKB
DNA replication initiation Source: UniProtKB
G1/S transition of mitotic cell cycle Source: UniProtKB
Negative regulation of cAMP-mediated signaling Source: UniProtKB
Positive regulation of inclusion body assembly Source: Ensembl
Positive regulation of neuron apoptotic process Source: Ensembl
Proteasome-mediated ubiquitin-dependent protein catabolic process Source: UniProtKB
Protein K48-linked ubiquitination Source: UniProtKB
Protein polyubiquitination Source: UniProtKB
Protein ubiquitination Source: UniProtKB
Response to growth factor Source: Ensembl
Ubiquitin-dependent protein catabolic process Source: GO_Central

Cellular Location

Nucleus; Cytoplasm. The phosphorylation of the C-terminal tail plays an important role in mediating nuclear localization. Colocalizes with beta-tubulin on mitotic spindles in anaphase.

PTM

Autoubiquitinated (PubMed:22496338, PubMed:11805320, PubMed:12060736). Autoubiquitination is promoted by the human herpes virus 1 protein ICP0 and leads to degradation by the Ubiquitin-proteasomal pathway (PubMed:11805320, PubMed:12060736).
Phosphorylated by CK2. Phosphorylation of the C-terminal tail by CK2 controles the nuclear localization.

More Infomation

References

Zhao, X. C., Wang, G. Z., Wen, Z. S., Zhou, Y. C., Hu, Q., Zhang, B., ... & Zhou, G. B. (2020). Systematic identification of CDC34 that functions to stabilize EGFR and promote lung carcinogenesis. EBioMedicine, 53, 102689.

Williams, K. M., Qie, S., Atkison, J. H., Salazar-Arango, S., Diehl, J. A., & Olsen, S. K. (2019). Structural insights into E1 recognition and the ubiquitin-conjugating activity of the E2 enzyme Cdc34. Nature communications, 10(1), 1-15.

Liu, X., Zhang, Y., Hu, Z., Li, Q., Yang, L., & Xu, G. (2018). The catalytically inactive mutation of the ubiquitin-conjugating enzyme CDC34 affects its stability and cell proliferation. The protein journal, 37(2), 132-143.

Zhao, X. C., Wang, G. Z., Zhou, Y. C., Ma, L., Liu, J., Zhang, C., ... & Zhou, G. B. (2018). Genome-wide identification of CDC34 that stabilizes EGFR and promotes lung carcinogenesis. BioRxiv, 255844.

Takagi, K., Takayama, T., Midorikawa, Y., Hasegawa, H., Ochiai, T., Moriguchi, M., ... & Fujiwara, K. (2017). Cell division cycle 34 is highly expressed in hepatitis C virus-positive hepatocellular carcinoma with favorable phenotypes. Biomedical reports, 7(1), 41-46.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Associated Products

Related Products

Rabbit Anti-CDC34 Recombinant Antibody (EG567)

Mouse Anti-CDC34 Recombinant Antibody (C2991)

Mouse Anti-CDC34 Recombinant Antibody (CBFYC-001)

Mouse Anti-CDC34 Recombinant Antibody (CBFYC-1516)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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