Search :
Sign in or Register  
Welcome Sign in or Don't have an account?Register

Mouse Anti-CDC20 Recombinant Antibody (CBYC-XB004) (CBMAB-XB0216-YC)


See all CDC20 antibodies
Online Inquiry
Published Data
CBMAB-XB0216-YC-1
Mouse Anti-CDC20 Recombinant Antibody (CBYC-XB004) (CBMAB-XB0216-YC) in WB
MBP or MBP-MAD1 (wild-type or mutant):MAD2 is immobilized on amylose beads and serves as baits to pull down prey including O-MAD2 (a V193N mutant that stabilizes MAD2 in the open conformation), MPS1-phosphorylated BUB1:BUB3, and CDC20. From top to bottom: a Coomassie-stained SDS-PAGE gel, an immunoblot detecting BUB1, and an immunoblot detecting CDC20. ...View More
Chen, C., Piano, V., Alex, A., Han, S. J., Huis in't Veld, P. J., Roy, B., ... & Joglekar, A. P. (2023). The structural flexibility of MAD1 facilitates the assembly of the mitotic checkpoint complex. Nature Communications, 14(1), 1529.
Distributed under Open Access license CC BY 4.0, without modification.
CBMAB-XB0216-YC-2
Mouse Anti-CDC20 Recombinant Antibody (CBYC-XB004) (CBMAB-XB0216-YC) in Immunoblot and IP
HeLa Tet-On cells stably expressing Flag-Cdc20 WT or 5A were arrested in mitosis by 5 μM nocodazole. The cell lysates and the anti-Flag immunoprecipitates (IP) of these cells were blotted with the indicated antibodies. The graphs at the bottom show the quantification of the relative BubR1 and Mad2 signals normalized to that of total Cdc20 in the IP (mean±range; n=2 independent experiments). ...View More
Jia, L., Li, B., & Yu, H. (2016). The Bub1-Plk1 kinase complex promotes spindle checkpoint signalling through Cdc20 phosphorylation. Nature communications, 7(1), 10818.
Distributed under Open Access license CC BY 4.0, without modification.
CBMAB-XB0216-YC-3
Mouse Anti-CDC20 Recombinant Antibody (CBYC-XB004) (CBMAB-XB0216-YC) in Immunoblot
Undifferentiated C2C12 myoblasts (undiff) or differentiated C2C12 myotubes (diff) were infected with Ade-LacZ or Ade-YAP(S127A). After 2 days, the expression of the indicated proteins was analyzed by immunoblotting. ...View More
Gründl, M., Walz, S., Hauf, L., Schwab, M., Werner, K. M., Spahr, S., ... & Gaubatz, S. (2020). Interaction of YAP with the Myb-MuvB (MMB) complex defines a transcriptional program to promote the proliferation of cardiomyocytes. PLoS Genetics, 16(5), e1008818.
Distributed under Open Access license CC BY 4.0, without modification.

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
CBYC-XB004
Antibody Isotype
IgG2b, κ
Application
WB, IF, IP, ELISA, IHC-P

Basic Information

Immunogen
Amino acids 1-175 of Cdc20 of human.
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG2b, κ
Clonality
Monoclonal Antibody
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
ELISA1:100-1:1,000
IHC-P1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Cell Division Cycle 20
Introduction
CDC20 (Cell Division Cycle 20) is a protein coding gene. Diseases associated with CDC20 include Uterine Corpus Endometrial Carcinoma. Among its related pathways are Metabolism of proteins and Innate Immune System. Gene Ontology annotations related to this gene include enzyme binding and protein C-terminus binding. An important paralog of this gene is FZR1.
Entrez Gene ID
991
UniProt ID
Q12834
Alternative Names
Cell Division Cycle 20; P55CDC; CDC20 (Cell Division Cycle 20, S. Cerevisiae, Homolog); CDC20 Cell Division Cycle 20 Homolog (S. Cerevisiae); Cell Division Cycle 20 Homolog (S. Cerevisiae); Cell Division Cycle Protein 20 Homolog;
Function
Required for full ubiquitin ligase activity of the anaphase promoting complex/cyclosome (APC/C) and may confer substrate specificity upon the complex. Is regulated by MAD2L1: in metaphase the MAD2L1-CDC20-APC/C ternary complex is inactive and in anaphase the CDC20-APC/C binary complex is active in degrading substrates. The CDC20-APC/C complex positively regulates the formation of synaptic vesicle clustering at active zone to the presynaptic membrane in postmitotic neurons. CDC20-APC/C-induced degradation of NEUROD2 induces presynaptic differentiation.
Biological Process
Anaphase-promoting complex-dependent catabolic process Source: UniProtKB
Cell differentiation Source: UniProtKB-KW
Cell division Source: UniProtKB-KW
Mitotic sister chromatid cohesion Source: Ensembl
Mitotic spindle assembly Source: Ensembl
Mitotic spindle assembly checkpoint Source: Reactome
Mitotic spindle organization Source: Reactome
Nervous system development Source: UniProtKB-KW
Positive regulation of anaphase-promoting complex-dependent catabolic process Source: GO_Central
Positive regulation of cell population proliferation Source: Ensembl
Positive regulation of synapse maturation Source: UniProtKB
Positive regulation of synaptic plasticity Source: UniProtKB
Positive regulation of ubiquitin protein ligase activity Source: UniProtKB
Protein deubiquitination Source: Reactome
Protein ubiquitination Source: UniProtKB-UniPathway
Regulation of dendrite development Source: Ensembl
Regulation of exit from mitosis Source: Reactome
Regulation of meiotic nuclear division Source: Ensembl
Regulation of mitotic cell cycle phase transition Source: Reactome
Ubiquitin-dependent protein catabolic process Source: Reactome
Cellular Location
Centrosome; Spindle pole
PTM
Acetylated. Deacetylated at Lys-66 by SIRT2; deacetylation enhances the interaction of CDC20 with CDC27, leading to activation of anaphase promoting complex/cyclosome (APC/C).
Phosphorylated during mitosis, probably by maturation promoting factor (MPF). Phosphorylated by BUB1 at Ser-41; Ser-72; Ser-92; Ser-153; Thr-157 and Ser-161. Phosphorylated by NEK2.
Dephosphorylated by CTDP1.
Ubiquitinated and degraded by the proteasome during spindle assembly checkpoint. Deubiquitinated by USP44, leading to stabilize the MAD2L1-CDC20-APC/C ternary complex, thereby preventing premature activation of the APC/C. Ubiquitinated at Lys-490 during prometaphase. Ubiquitination at Lys-485 and Lys-490 has no effect on its ability to bind the APC/C complex.
More Infomation

Gao, Y., Bai, L., & Shang, G. (2021). Notch-1 promotes the malignant progression of osteosarcoma through the activation of cell division cycle 20. Aging (Albany NY), 13(2), 2668.

Shen, P., He, X., Lan, L., Hong, Y., & Lin, M. (2020). Identification of cell division cycle 20 as a candidate biomarker and potential therapeutic target in bladder cancer using bioinformatics analysis. Bioscience reports, 40(7), BSR20194429.

Qiu, E., Gao, Y., Zhang, B., Xia, T., Zhang, Z., & Shang, G. (2020). Upregulation of cell division cycle 20 in cisplatin resistance-induced epithelial-mesenchymal transition in osteosarcoma cells. American journal of translational research, 12(4), 1309.

Huang, P., Le, X., Huang, F., Yang, J., Yang, H., Ma, J., ... & Chen, Z. (2020). Discovery of a dual tubulin polymerization and cell division cycle 20 homologue inhibitor via structural modification on apcin. Journal of medicinal chemistry, 63(9), 4685-4700.

Zhang, Q., Huang, H., Liu, A., Li, J., Liu, C., Sun, B., ... & Su, C. (2019). Cell division cycle 20 (CDC20) drives prostate cancer progression via stabilization of β-catenin in cancer stem-like cells. EBioMedicine, 42, 397-407.

Cheng, S., Castillo, V., & Sliva, D. (2019). CDC20 associated with cancer metastasis and novel mushroom‑derived CDC20 inhibitors with antimetastatic activity. International journal of oncology, 54(6), 2250-2256.

Chu, Z., Zhang, X., Li, Q., Hu, G., Lian, C. G., & Geng, S. (2019). CDC20 contributes to the development of human cutaneous squamous cell carcinoma through the Wnt/β‑catenin signaling pathway. International journal of oncology, 54(5), 1534-1544.

Shang, G., Ma, X., & Lv, G. (2018). Cell division cycle 20 promotes cell proliferation and invasion and inhibits apoptosis in osteosarcoma cells. Cell Cycle, 17(1), 43-52.

Zhang, Y., Xue, Y. B., Li, H., Qiu, D., Wang, Z. W., & Tan, S. S. (2017). Inhibition of cell survival by curcumin is associated with downregulation of cell division cycle 20 (Cdc20) in pancreatic cancer cells. Nutrients, 9(2), 109.

Ask a question We look forward to hearing from you.
0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Loading...
Have you used Mouse Anti-CDC20 Recombinant Antibody (CBYC-XB004)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon $30 eGift Card
Submit a review
Loading...
For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Learn more

Documents

Request bulk or custom quoteOnline Inquiry
Second antibody and isotype control

Contact us

  • Tel: (USA)
  • (UK)
  • Fax:
  • Email:

Submit A Review

Online Inquiry