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Mouse Anti-CD99 Recombinant Antibody (CBFYC-1504) (CBMAB-C1563-FY)


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Published Data
CBMAB-C1563-FY-1
Mouse Anti-CD99 Recombinant Antibody (CBFYC-1504) (CBMAB-C1563-FY) in WB
Representative Western blot depicting the expression of CD99, GPI, and IGFALS in EVs extracted from CD99pos and CD99neg TC-71 cells. GAPDH was used as loading control. ...View More
De Feo, A., Manfredi, M., Mancarella, C., Maqueda, J. J., De Giorgis, V., Pignochino, Y., ... & Scotlandi, K. (2024). CD99 Modulates the Proteomic Landscape of Ewing Sarcoma Cells and Related Extracellular Vesicles. International Journal of Molecular Sciences, 25(3), 1588.
Distributed under Open Access license CC BY 4.0, without modification.
CBMAB-C1563-FY-2
Mouse Anti-CD99 Recombinant Antibody (CBFYC-1504) (CBMAB-C1563-FY) in FC
Szie distribution of vesicles derived from EWS cells lines and CD99-silenced cells (TC-71 and TC-CD99-shRNA, IOR/CAR and CAR-CD99-shRNA) analyzed by the Nanosight technology. ...View More
De Feo, A., Sciandra, M., Ferracin, M., Felicetti, F., Astolfi, A., Pignochino, Y., ... & Scotlandi, K. (2019). Exosomes from CD99-deprived Ewing sarcoma cells reverse tumor malignancy by inhibiting cell migration and promoting neural differentiation. Cell death & disease, 10(7), 471.
Distributed under Open Access license CC BY 4.0, without modification.
CBMAB-C1563-FY-3
Mouse Anti-CD99 Recombinant Antibody (CBFYC-1504) (CBMAB-C1563-FY) in WB
Representative western blotting showing CD99 secreted in the supernatants of eight EWS cell lines; CD99 protein expression in the corresponding EWS cells is shown for comparison. GAPDH was used as a loading control. ...View More
De Feo, A., Sciandra, M., Ferracin, M., Felicetti, F., Astolfi, A., Pignochino, Y., ... & Scotlandi, K. (2019). Exosomes from CD99-deprived Ewing sarcoma cells reverse tumor malignancy by inhibiting cell migration and promoting neural differentiation. Cell death & disease, 10(7), 471.
Distributed under Open Access license CC BY 4.0, without modification.
CBMAB-C1563-FY-4
Mouse Anti-CD99 Recombinant Antibody (CBFYC-1504) (CBMAB-C1563-FY) in WB
Human myeloma cell lines (HMCLs) inhibit CD99 expression on normal human osteoblasts (hOBs). Human undifferentiated osteoblasts or hOBs differentiated for 2, 7, 14, or 21 days (Days of diff.), were cultured in the presence or absence of H929 (a) or U266 (b) HMCLs or their conditioned medium, and then were analyzed for western blot analysis to detect the protein levels of CD99. The histograms represent the mean optical density (OD) of CD99 ratio normalized to the OD of β-Actin. ...View More
Oranger, A., Brunetti, G., Carbone, C., Colaianni, G., Mongelli, T., Gigante, I., ... & Grano, M. (2015). Human myeloma cell lines induce osteoblast downregulation of CD99 which is involved in osteoblast formation and activity. Journal of Immunology Research, 2015(1), 156787.
Distributed under Open Access license CC BY 4.0, without modification.

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBFYC-1504
Antibody Isotype
IgG1, κ
Application
WB, IF, IP, IHC-P

Basic Information

Immunogen
Peptide from the MIC2.
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG1, κ
Clonality
Monoclonal Antibody
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
IHC-P1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
CD99 Molecule (Xg Blood Group)
Introduction
CD99 (CD99 Molecule (Xg Blood Group)) is a Protein Coding gene. Diseases associated with CD99 include Extraosseous Ewing's Sarcoma and Ewing Sarcoma. Among its related pathways are Response to elevated platelet cytosolic Ca2+ and Cell surface interactions at the vascular wall.
Entrez Gene ID
4267
UniProt ID
P14209
Alternative Names
CD99 Molecule (Xg Blood Group); CD99 Antigen; Antigen Identified By Monoclonal Antibodies 12E7, F21 And O13; T-Cell Surface Glycoprotein E2; E2 Antigen; MIC2X; MIC2Y; MIC2; Antigen Identified By Monoclonal 12E7, Y Homolog; MIC2 (Monoclonal Antibody 12E7)
Function
Involved in T-cell adhesion processes and in spontaneous rosette formation with erythrocytes. Plays a role in a late step of leukocyte extravasation helping leukocytes to overcome the endothelial basement membrane. Acts at the same site as, but independently of, PECAM1. Involved in T-cell adhesion processes (By similarity).
Biological Process
Homotypic cell-cell adhesion Source: GO_Central
Positive regulation of neutrophil extravasation Source: GO_Central
Regulation of immune response Source: Reactome
T cell extravasation Source: GO_Central
Cellular Location
Membrane
Topology
Extracellular: 23-122
Helical: 123-147
Cytoplasmic: 148-185
PTM
Extensively O-glycosylated.
More Infomation

Siwy, J., Wendt, R., Albalat, A., He, T., Mischak, H., Mullen, W., ... & Beige, J. (2021). CD99 and polymeric immunoglobulin receptor peptides deregulation in critical COVID‐19: A potential link to molecular pathophysiology?. Proteomics, 21(20), 2100133.

Mattisson, J., Danielsson, M., Hammond, M., Davies, H., Gallant, C. J., Nordlund, J., ... & Forsberg, L. A. (2021). Leukocytes with chromosome Y loss have reduced abundance of the cell surface immunoprotein CD99. Scientific Reports, 11(1), 1-9.

Takheaw, N., Sittithumcharee, G., Kariya, R., Kasinrerk, W., & Okada, S. (2021). Anti-human CD99 antibody exerts potent antitumor effects in mantle cell lymphoma. Cancer Immunology, Immunotherapy, 70(6), 1557-1567.

Vaikari, V. P., Park, M., Keossayan, L., MacKay, J. A., & Alachkar, H. (2020). Anti-CD99 scFv-ELP nanoworms for the treatment of acute myeloid leukemia. Nanomedicine: Nanotechnology, Biology and Medicine, 29, 102236.

Wu, J., Zhang, L., Li, H., Wu, S., & Liu, Z. (2019). Nrf2 induced cisplatin resistance in ovarian cancer by promoting CD99 expression. Biochemical and biophysical research communications, 518(4), 698-705.

Cardoso, L. C., Soares, R. D. S., Laurentino, T. D. S., Lerario, A. M., Marie, S. K., & Oba-Shinjo, S. M. (2019). CD99 expression in glioblastoma molecular subtypes and role in migration and invasion. International journal of molecular sciences, 20(5), 1137.

Pasello, M., Manara, M. C., & Scotlandi, K. (2018). CD99 at the crossroads of physiology and pathology. Journal of cell communication and signaling, 12(1), 55-68.

Çelik, H., Sciandra, M., Flashner, B., Gelmez, E., Kayraklıoğlu, N., Allegakoen, D. V., ... & Üren, A. (2018). Clofarabine inhibits Ewing sarcoma growth through a novel molecular mechanism involving direct binding to CD99. Oncogene, 37(16), 2181-2196.

Manara, M. C., Pasello, M., & Scotlandi, K. (2018). CD99: a cell surface protein with an oncojanus role in tumors. Genes, 9(3), 159.

Yeh, C. C., Chang, C. J., Twu, Y. C., Chu, C. C., Liu, B. S., Huang, J. T., ... & Yu, L. C. (2018). The molecular genetic background leading to the formation of the human erythroid-specific Xga/CD99 blood groups. Blood advances, 2(15), 1854-1864.

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For research use only. Not intended for any clinical use.

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