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Mouse Anti-CD69 Recombinant Antibody (CBYY-C0635) (CBMAB-C1924-YY)


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Published Data
CBMAB-C1924-YY-1
Mouse Anti-CD69 Recombinant Antibody (CBYY-C0635) (CBMAB-C1924-YY) in IF
Colocalization of CD3 and NKp46 and CD3 and CD69 in the perihematomal regions on day 3 after collagenase-induced ICH. CD3 immunoreactivity is shown in green; NKp46 is shown in red; CD69 immunoreactivity is shown in red. Sections were stained with DAPI (blue) to label the nuclei. The insets show a higher magnificence of immunoreactive cells in the corresponding merged images. Scale bar = 100 μm. ...View More
Zhang, Z., Li, Y., Shi, J., Zhu, L., Dai, Y., Fu, P., ... & Jiang, C. (2023). Lymphocyte-related immunomodulatory therapy with siponimod (BAF-312) improves outcomes in mice with acute intracerebral hemorrhage. Aging and Disease, 14(3), 966. Distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
CBMAB-C1924-YY-2
Mouse Anti-CD69 Recombinant Antibody (CBYY-C0635) (CBMAB-C1924-YY) in FC
Flow cytometry plots show the expression of CD69 and perforin in NK cells obtained from blood and brain tissues of ICH mice. Gates were set using fluorescence minus one (FMO) controls. n = 12/group. ...View More
Li, Z., Li, M., Shi, S. X., Yao, N., Cheng, X., Guo, A., ... & Liu, Q. (2020). Brain transforms natural killer cells that exacerbate brain edema after intracerebral hemorrhage. Journal of Experimental Medicine, 217(12), e20200213. Distributed under Open Access license CC BY 4.0, without modification.
CBMAB-C1924-YY-3
Mouse Anti-CD69 Recombinant Antibody (CBYY-C0635) (CBMAB-C1924-YY) in IF
Immunostaining of CD56+NKp46+ cells, CD57+NKp46+ cells, CD69+NKp46+ cells, and perforin+NKp46+ cells in perihematomal region within 12 h after ICH or controls. Postmortem brain tissues from patients without history of neurological diseases were used as controls. Scale bars: 50 µm. ...View More
Li, Z., Li, M., Shi, S. X., Yao, N., Cheng, X., Guo, A., ... & Liu, Q. (2020). Brain transforms natural killer cells that exacerbate brain edema after intracerebral hemorrhage. Journal of Experimental Medicine, 217(12), e20200213. Distributed under Open Access license CC BY 4.0, without modification.
CBMAB-C1924-YY-4
Mouse Anti-CD69 Recombinant Antibody (CBYY-C0635) (CBMAB-C1924-YY) in WB
The CD69 level detected by western blot analysis. ...View More
Zhou, X., Zhang, P., Luo, W., Zhang, L., Hu, R., Sun, Y., & Jiang, H. (2018). Ketamine induces apoptosis in lung adenocarcinoma cells by regulating the expression of CD 69. Cancer Medicine, 7(3), 788-795. Distributed under Open Access license CC BY 4.0, without modification.

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
CBYY-C0635
Antibody Isotype
IgG1, κ
Application
WB, IF, IP, ELISA

Basic Information

Immunogen
Amino acids 1-199 representing full length CD69 of human origin.
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG1, κ
Clonality
Monoclonal Antibody
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
ELISA1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
CD69 Molecule
Entrez Gene ID
969
UniProt ID
Q07108
Function
Involved in lymphocyte proliferation and functions as a signal transmitting receptor in lymphocytes, natural killer (NK) cells, and platelets.
Cellular Location
Membrane
Topology
Cytoplasmic: 1-40
Helical: 41-61
Extracellular: 62-199
PTM
Constitutive Ser/Thr phosphorylation in both mature thymocytes and activated T-lymphocytes.
More Infomation

Gorabi, A. M., Hajighasemi, S., Kiaie, N., Hayat, S. M. G., Jamialahmadi, T., Johnston, T. P., & Sahebkar, A. (2020). The pivotal role of CD69 in autoimmunity. Journal of autoimmunity, 111, 102453.

Notario, L., Redondo-Antón, J., Alari-Pahissa, E., Albentosa, A., Leiva, M., Lopez, D., ... & Lauzurica, P. (2019). CD69 targeting enhances anti-vaccinia virus immunity. Journal of virology, 93(19), e00553-19.

Kimura, M. Y., Koyama-Nasu, R., Yagi, R., & Nakayama, T. (2019, May). A new therapeutic target: the CD69-Myl9 system in immune responses. In Seminars in immunopathology (Vol. 41, No. 3).

Walsh, D. A., Da Silva, H. B., Beura, L. K., Peng, C., Hamilton, S. E., Masopust, D., & Jameson, S. C. (2019). The functional requirement for CD69 in establishment of resident memory CD8+ T cells varies with tissue location. The Journal of Immunology, 203(4), 946-955.

Brait, V. H., Miró-Mur, F., Pérez-de-Puig, I., Notario, L., Hurtado, B., Pedragosa, J., ... & Planas, A. M. (2019). CD69 plays a beneficial role in ischemic stroke by dampening endothelial activation. Circulation research, 124(2), 279-291.

Mita, Y., Kimura, M. Y., Hayashizaki, K., Koyama-Nasu, R., Ito, T., Motohashi, S., ... & Nakayama, T. (2018). Crucial role of CD69 in anti-tumor immunity through regulating the exhaustion of tumor-infiltrating T cells. International immunology, 30(12), 559-567.

Kimura, M. Y., Igi, A., Hayashizaki, K., Mita, Y., Shinzawa, M., Kadakia, T., ... & Nakayama, T. (2018). CD69 prevents PLZF hi innate precursors from prematurely exiting the thymus and aborting NKT2 cell differentiation. Nature communications, 9(1), 1-12.

Cibrián, D., & Sánchez‐Madrid, F. (2017). CD69: from activation marker to metabolic gatekeeper. European journal of immunology, 47(6), 946-953.

Yong, Y. K., Tan, H. Y., Saeidi, A., Rosmawati, M., Atiya, N., Ansari, A. W., ... & Shankar, E. M. (2017). Decrease of CD69 levels on TCR Vα7. 2+ CD4+ innate-like lymphocytes is associated with impaired cytotoxic functions in chronic hepatitis B virus-infected patients. Innate immunity, 23(5), 459-467.

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For research use only. Not intended for any clinical use.

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