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Rat Anti-CD4 Recombinant Antibody (AG30) (V2LY-0524-LY108)


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Summary

Host Animal
Rat
Specificity
Mouse
Clone
AG30
Antibody Isotype
IgG2b, κ
Application
FC, IHC-F, In Vivo, WB

Basic Information

Immunogen
Unknown
Host Species
Rat
Specificity
Mouse
Antibody Isotype
IgG2b, κ
Clonality
Monoclonal Antibody

Formulations & Storage [For reference only, actual COA shall prevail!]

Endotoxin
< 1.0 EU/mg as determined by the LAL method
Format
Liquid
Buffer
PBS
Preservative
None
Concentration
5 mg/ml
Purity
>95% by SDS Page
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
CD4 Molecule
Entrez Gene ID
12504
UniProt ID
P06332
Alternative Names
CD4 Molecule; T-Cell Surface Glycoprotein CD4; T-Cell Surface Antigen T4/Leu-3; CD4 Antigen (P55); CD4 Receptor; CD4 Antigen; CD4mut;
Function
Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class II molecule:peptide complex. The antigens presented by class II peptides are derived from extracellular proteins while class I peptides are derived from cytosolic proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class II presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of T-helper cells. In other cells such as macrophages or NK cells, plays a role in differentiation/activation, cytokine expression and cell migration in a TCR/LCK-independent pathway. Participates in the development of T-helper cells in the thymus and triggers the differentiation of monocytes into functional mature macrophages.
(Microbial infection) Primary receptor for human immunodeficiency virus-1 (HIV-1) (PubMed:2214026, PubMed:16331979, PubMed:9641677, PubMed:12089508).
Down-regulated by HIV-1 Vpu (PubMed:17346169).
Acts as a receptor for Human Herpes virus 7/HHV-7 (PubMed:7909607).
Biological Process
Adaptive immune response Source: UniProtKB-KW
Cell adhesion Source: InterPro
Cell surface receptor signaling pathway Source: ProtInc
Cellular response to granulocyte macrophage colony-stimulating factor stimulus Source: CAFA
Cytokine-mediated signaling pathway Source: Reactome
Defense response to Gram-negative bacterium Source: Ensembl
Entry into host Source: Reactome
Enzyme linked receptor protein signaling pathway Source: ProtInc
Fusion of virus membrane with host plasma membrane Source: Reactome
Helper T cell enhancement of adaptive immune response Source: Ensembl
Immune response Source: UniProtKB
Induction by virus of host cell-cell fusion Source: UniProtKB
Interleukin-15-mediated signaling pathway Source: CAFA
Macrophage differentiation Source: ARUK-UCL
Maintenance of protein location in cell Source: MGI
Membrane organization Source: Reactome
Positive regulation of calcium ion transport into cytosol Source: Ensembl
Positive regulation of calcium-mediated signaling Source: Ensembl
Positive regulation of ERK1 and ERK2 cascade Source: CAFA
Positive regulation of I-kappaB kinase/NF-kappaB signaling Source: CAFA
Positive regulation of interleukin-2 production Source: UniProtKB
Positive regulation of kinase activity Source: CAFA
Positive regulation of MAPK cascade Source: CAFA
Positive regulation of monocyte differentiation Source: CAFA
Positive regulation of peptidyl-tyrosine phosphorylation Source: Ensembl
Positive regulation of protein kinase activity Source: UniProtKB
Positive regulation of protein phosphorylation Source: CAFA
Positive regulation of T cell proliferation Source: Ensembl
Positive regulation of transcription, DNA-templated Source: CAFA
Positive regulation of viral entry into host cell Source: CAFA
Regulation of calcium ion transport Source: CAFA
Regulation of defense response to virus by virus Source: Reactome
Regulation of T cell activation Source: UniProtKB
Response to estradiol Source: Ensembl
Response to vitamin D Source: Ensembl
Signal transduction Source: ProtInc
T cell activation Source: GO_Central
T cell differentiation Source: UniProtKB
T cell receptor signaling pathway Source: Reactome
T cell selection Source: UniProtKB
Transmembrane receptor protein tyrosine kinase signaling pathway Source: UniProtKB
Cellular Location
Cell membrane. Localizes to lipid rafts (PubMed:12517957, PubMed:9168119). Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum.
Topology
Extracellular: 26-396
Helical: 397-418
Cytoplasmic: 419-458
PTM
Palmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts.
Phosphorylated by PKC; phosphorylation at Ser-433 plays an important role for CD4 internalization.
More Infomation

Xing, J., Luo, K., Tang, X., & Zhan, W. (2019). Influence of CD4-1+, CD4-2+ and CD8+ T lymphocytes subpopulations on the immune response of B lymphocytes in flounder (Paralichthys olivaceus) immunized with thymus-dependent or thymus-independent antigen. Fish & shellfish immunology, 84, 979-986.

Taniuchi, I. (2018). CD4 helper and CD8 cytotoxic T cell differentiation. Annual review of immunology, 36, 579-601.

Dhanda, S. K., Karosiene, E., Edwards, L., Grifoni, A., Paul, S., Andreatta, M., ... & Sette, A. (2018). Predicting HLA CD4 immunogenicity in human populations. Frontiers in Immunology, 9, 1369.

Prasad, S., Neef, T., Xu, D., Podojil, J. R., Getts, D. R., Shea, L. D., & Miller, S. D. (2018). Tolerogenic Ag-PLG nanoparticles induce tregs to suppress activated diabetogenic CD4 and CD8 T cells. Journal of autoimmunity, 89, 112-124.

Sun, G., Sun, X., Li, W., Liu, K., Tian, D., Dong, Y., ... & Zhang, D. (2018). Critical role of OX40 in the expansion and survival of CD4 T-cell-derived double-negative T cells. Cell death & disease, 9(6), 1-13.

Liu, Q., Acharya, P., Dolan, M. A., Zhang, P., Guzzo, C., Lu, J., ... & Lusso, P. (2017). Quaternary contact in the initial interaction of CD4 with the HIV-1 envelope trimer. Nature structural & molecular biology, 24(4), 370-378.

Michels, A. W., Landry, L. G., McDaniel, K. A., Yu, L., Campbell-Thompson, M., Kwok, W. W., ... & Nakayama, M. (2017). Islet-derived CD4 T cells targeting proinsulin in human autoimmune diabetes. Diabetes, 66(3), 722-734.

Hamze, M., Meunier, S., Karle, A., Gdoura, A., Goudet, A., Szely, N., ... & Maillère, B. (2017). Characterization of CD4 T cell epitopes of infliximab and rituximab identified from healthy donors. Frontiers in immunology, 8, 500.

Takeuchi, A., & Saito, T. (2017). CD4 CTL, a cytotoxic subset of CD4+ T cells, their differentiation and function. Frontiers in immunology, 8, 194.

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For research use only. Not intended for any clinical use.

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