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Mouse Anti-CD3E Recombinant Antibody (CBHb201) (CBMAB-Hb270-LY)


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Published Data
CBMAB-Hb270-LY-1
Mouse Anti-CD3E Recombinant Antibody (CBHb201) (CBMAB-Hb270-LY) in IHC
F4/80 and CD3 positive cell number in SCC tumours were unaffected by UE2316.
Representative images of squamous cell carcinoma (SCC) tumours from control (A) and UE2316-treated (B) mice are shown, with DAB immunoreactivity to anti-F4/80 antibody shown in brown and haematoxylin-counterstained nuclei in blue. C) Immunostaining did not reveal a difference in F4/80-positive stain area between tumour from control and UE2316-treated mice (P = 0.17). N = 5-6/group. Data were compared by independent samples t-test. Scale bar = 50μm. Immunostaining found no difference in CD3-positive stain area between SCC tumours from control and UE2316-treated mice, assessed by whole section analysis. N = 5/group (D). Representative images of CD3 labelled SCC tumour sections from control (E) and UE2316-treated (F) squamous cell carcinoma (SCC). DAB immunoreactivity shown in brown and haematoxylin-counterstained nuclei in blue. Data were compared by independent samples t-test, N = 5/group. ...View More
Davidson, C. T., Miller, E., Muir, M., Dawson, J. C., Lee, M., Aitken, S., ... & Walker, B. R. (2023). 11β-HSD1 inhibition does not affect murine tumour angiogenesis but may exert a selective effect on tumour growth by modulating inflammation and fibrosis. Plos one, 18(3), e0255709. Distributed under Open Access license CC BY 4.0, without modification.
CBMAB-Hb270-LY-2
Mouse Anti-CD3E Recombinant Antibody (CBHb201) (CBMAB-Hb270-LY) in IHC
Immunohistochemically stained sections of the spleen with CD3 (A,C,E, and G) and CD68 (B,D,F, and, H) from different groups. (A) control group showed strong CD3 immunostaining, (C) LA group showed weak CD3 immunostaining, e and g; LA + CIN, and LA + CUR groups showed moderate CD3 immunostaining, (B) and (H); control and LA + CUR groups showed weak CD68, (D); LA group showed strong CD68 immunostaining, and (F); LA + CIN group showed moderate CD68 immunostaining. ...View More
Emam, M. A., Farouk, S. M., Aljazzar, A., Abdelhameed, A. A., Eldeeb, A. A., & Gad, F. A. M. (2023). Curcumin and cinnamon mitigates lead acetate-induced oxidative damage in the spleen of rats. Frontiers in Pharmacology, 13, 1072760. Distributed under Open Access license CC BY 4.0, without modification.
CBMAB-Hb270-LY-3
Mouse Anti-CD3E Recombinant Antibody (CBHb201) (CBMAB-Hb270-LY) in IHC
Expression levels and statistical analysis of CD3 in skin tissues from 20-week-old ApoE-/- mice induced by ND and HFD by immunohistochemical staining (n = 3). ...View More
Liang, Y., Gu, T., Peng, S., Lin, Y., Liu, J., Wang, X., ... & Lin, J. (2022). p16INK4a Plays Critical Role in Exacerbating Inflammaging in High Fat Diet Induced Skin. Oxidative Medicine and Cellular Longevity, 2022(1), 3415528. Distributed under Open Access license CC BY 4.0, without modification.

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
CBHb201
Antibody Isotype
IgG1, κ
Application
WB, IP, FC, IF, IHC-P

Basic Information

Immunogen
Synthetic peptide spanning amino acids 156-168 of CD3 of human CD3-ε chain.
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG1, κ
Clonality
Monoclonal Antibody
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
IHC-P1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Cluster Of Differentiation 3
Alternative Names
CD3
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For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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