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Rat Anti-CCR5 Recombinant Antibody (HEK/1/85a) (CBMAB-0172CQ)


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Summary

Host Animal
Rat
Specificity
Human
Clone
HEK/1/85a
Antibody Isotype
IgG2a
Application
FC

Basic Information

Immunogen
CHO cells transfected with human CCR5.
Host Species
Rat
Specificity
Human
Antibody Isotype
IgG2a
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
FC1:50-1:100

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS
Preservative
0.09% sodium azide
Concentration
1 mg/ml
Purity
>95% as determined by analysis by SDS-PAGE
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
C-C Motif Chemokine Receptor 5 (Gene/Pseudogene)
Introduction
This gene encodes a member of the beta chemokine receptor family, which is predicted to be a seven transmembrane protein similar to G protein-coupled receptors. By radiation hybrid mapping, Liu et al. (1996) localized the CCR5 gene (designated CKR5 by them) to chromosome 3p21. Defective alleles of this gene have been associated with the HIV infection resistance. May play a role in the control of granulocytic lineage proliferation or differentiation. Acts as a coreceptor (CD4 being the primary receptor) for HIV-1 R5 isolates. The ligands of this receptor include monocyte chemoattractant protein 2 (MCP-2), macrophage inflammatory protein 1 alpha (MIP-1 alpha), macrophage inflammatory protein 1 beta (MIP-1 beta) and regulated on activation normal T expressed and secreted protein (RANTES). Expression of this gene was also detected in a promyeloblastic cell line, suggesting that this protein may play a role in granulocyte lineage proliferation and differentiation.
Entrez Gene ID
Human1234
Mouse12774
Rat117029
UniProt ID
HumanP51681
MouseP51682
RatO08556
Alternative Names
C-C Motif Chemokine Receptor 5 (Gene/Pseudogene); Chemokine (C-C Motif) Receptor 5; HIV-1 Fusion Coreceptor; CC-CKR-5; ChemR13; CMKBR5; CCR-5; Chemokine (C-C Motif) Receptor 5 (Gene/Pseudogene); C-C Motif Chemokine Receptor 5 A159A; Chemokine Recptor CCR5 Delta32;
Function
Receptor for a number of inflammatory CC-chemokines including CCL3/MIP-1-alpha, CCL4/MIP-1-beta and RANTES and subsequently transduces a signal by increasing the intracellular calcium ion level. May play a role in the control of granulocytic lineage proliferation or differentiation. Participates in T-lymphocyte migration to the infection site by acting as a chemotactic receptor (PubMed:30713770).
(Microbial infection) Acts as a coreceptor (CD4 being the primary receptor) of human immunodeficiency virus-1/HIV-1.
Biological Process
Calcium ion transport Source: UniProtKB
Calcium-mediated signaling Source: UniProtKB
Cell-cell signaling Source: UniProtKB
Cell chemotaxis Source: GO_Central
Cell surface receptor signaling pathway Source: ProtInc
Cellular defense response Source: ProtInc
Cellular response to lipopolysaccharide Source: UniProtKB
Chemotaxis Source: ProtInc
Cytokine-mediated signaling pathway Source: Reactome
Dendritic cell chemotaxis Source: BHF-UCL
Entry into host Source: Reactome
Fusion of virus membrane with host plasma membrane Source: Reactome
G protein-coupled receptor signaling pathway Source: UniProtKB
Immune response Source: GO_Central
Inflammatory response Source: GO_Central
MAPK cascade Source: UniProtKB
Positive regulation of cytosolic calcium ion concentration Source: GO_Central
Release of sequestered calcium ion into cytosol by sarcoplasmic reticulum Source: UniProtKB
Response to cholesterol Source: UniProtKB
Signaling Source: UniProtKB
Cellular Location
Cell membrane
Involvement in disease
Diabetes mellitus, insulin-dependent, 22 (IDDM22): A multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy. Clinical features are polydipsia, polyphagia and polyuria which result from hyperglycemia-induced osmotic diuresis and secondary thirst. These derangements result in long-term complications that affect the eyes, kidneys, nerves, and blood vessels.
Topology
Extracellular: 1-30
Helical: 31-58
Cytoplasmic: 59-68
Helical: 69-89
Extracellular: 90-102
Helical: 103-124
Cytoplasmic: 125-141
Helical: 142-166
Extracellular: 167-198
Helical: 199-218
Cytoplasmic: 219-235
Helical: 236-260
Extracellular: 261-277
Helical: 278-301
Cytoplasmic: 302-352
PTM
Sulfated on at least 2 of the N-terminal tyrosines. Sulfation contributes to the efficiency of HIV-1 entry and is required for efficient binding of the chemokines, CCL3 and CCL4.
O-glycosylated, but not N-glycosylated. Ser-6 appears to be the major site. Also sialylated glycans present which contribute to chemokine binding. Thr-16 and Ser-17 may also be glycosylated and, if so, with small moieties such as a T-antigen.
Palmitoylation in the C-terminal is important for cell surface expression, and to a lesser extent, for HIV entry.
Phosphorylation on serine residues in the C-terminal is stimulated by binding CC chemokines especially by APO-RANTES.
More Infomation

Liraz-Zaltsman, S., Friedman-Levi, Y., Shabashov-Stone, D., Gincberg, G., Atrakcy-Baranes, D., Joy, M. T., ... & Shohami, E. (2021). Chemokine Receptors CC Chemokine Receptor 5 and CXC Motif Chemokine Receptor 4 Are New Therapeutic Targets for Brain Recovery after Traumatic Brain Injury. Journal of Neurotrauma.

Wang, X., Li, W., Yue, Q., Du, W., Li, Y., Liu, F., ... & Hu, J. (2021). CC chemokine receptor 5 signaling contributes to cardiac remodeling and dysfunction under pressure overload. Molecular Medicine Reports, 23(1), 1-1.

Karakaya, B., van Moorsel, C. H., Veltkamp, M., Roodenburg-Benschop, C., Kazemier, K. M., van der Helm-van Mil, A., ... & Rijkers, G. T. (2021). A Polymorphism in CC Chemokine Receptor 5 (CCR5) Associates with Löfgren’s Syndrome and Alters Receptor Expression as well as Functional Response. Cells, 10(8), 1967.

Isaikina, P., Tsai, C. J., Dietz, N., Pamula, F., Grahl, A., Goldie, K. N., ... & Grzesiek, S. (2021). Structural basis of the activation of the CC chemokine receptor 5 by a chemokine agonist. Science Advances, 7(25), eabg8685.

Torretta, S., Colombo, G., Travelli, C., Boumya, S., Lim, D., Genazzani, A. A., & Grolla, A. A. (2020). The Cytokine Nicotinamide Phosphoribosyltransferase (eNAMPT; PBEF; Visfatin) Acts as a Natural Antagonist of CC Chemokine Receptor Type 5 (CCR5). Cells, 9(2), 496.

Zhang, Z., Wang, Q., Yao, J., Zhou, X., Zhao, J., Zhang, X., ... & Liao, L. (2020). Chemokine receptor 5, a double-edged sword in metabolic syndrome and cardiovascular disease. Frontiers in pharmacology, 11, 146.

Petti, L. M., Marlatt, S. A., Luo, Y., Scheideman, E. H., Shelar, A., & DiMaio, D. (2018). Regulation of CC chemokine receptor 5 (CCR5) stability by Lys197 and by transmembrane protein aptamers that target it for lysosomal degradation. Journal of Biological Chemistry, 293(23), 8787-8801.

Constanza Gomez Victoria, E., Cristina de Brito Toscano, E., Clara de Sousa Cardoso, A., Gonçalves da Silva, D., Silvade Miranda, A., da Silva Barcelos, L., ... & Alvarenga Rachid, M. (2017). Knockdown of CC chemokine receptor 5 (CCR5) is protective against cerebral ischemia and reperfusion injury. Current neurovascular research, 14(2), 125-131.

Zheng, Y., Han, G. W., Abagyan, R., Wu, B., Stevens, R. C., Cherezov, V., ... & Handel, T. M. (2017). Structure of CC chemokine receptor 5 with a potent chemokine antagonist reveals mechanisms of chemokine recognition and molecular mimicry by HIV. Immunity, 46(6), 1005-1017.

Zi, J., Yuan, S., Qiao, J., Zhao, K., Xu, L., Qi, K., ... & Zeng, L. (2017). Treatment with the CC chemokine receptor type 5 (CCR5)-inhibitor maraviroc suppresses growth and induces apoptosis of acute lymphoblastic leukemia cells. American journal of cancer research, 7(4), 869.

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For research use only. Not intended for any clinical use.

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