Mouse Anti-CCNJ Recombinant Antibody (CBFYC-1058) (CBMAB-C1115-FY)

Name: Mouse Anti-CCNJ Recombinant Antibody (CBFYC-1058)
SKU: CBMAB-C1115-FY
Rating: 5 (1 reviews)

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Summary

Host Animal

Mouse

Specificity

Human

Clone

CBFYC-1058

Antibody Isotype

IgG1

Application

IP, WB

Basic Information

Immunogen

Near the C-terminus of cyclin J of human origin.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG1

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.1 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name

Cyclin J

Introduction

CCNJ (Cyclin J) is a Protein Coding gene. An important paralog of this gene is CCNJL.

Entrez Gene ID

54619

UniProt ID

Q5T5M9

Alternative Names

Cyclin J; BA690P14.1; Cyclin-J

Biological Process

Mitotic cell cycle phase transition Source: GO_Central
Regulation of cyclin-dependent protein serine/threonine kinase activity Source: GO_Central

Cellular Location

Centrosome; Nucleus; Cyclin-dependent protein kinase holoenzyme complex; Cytoplasm

More Infomation

References

Zhang, J., Song, N., & Duan, Z. (2019). Rs6265 polymorphism in brain‐derived neurotrophic factor (Val/Val and Val/Met) promotes proliferation of bladder cancer cells by suppressing microRNA‐205 and enhancing expression of cyclin J. Journal of cellular biochemistry, 120(5), 7297-7308.

Shi, L., Xu, Z., Wu, G., Chen, X., Huang, Y., Wang, Y., ... & Ke, B. (2017). Up-regulation of miR-146a increases the sensitivity of non-small cell lung cancer to DDP by downregulating cyclin J. BMC cancer, 17(1), 1-14.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Associated Products

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Learn more

Documents

Datasheet
SDS
Request for COA

Request bulk or custom quote Online Inquiry
Second antibody and isotype control

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