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Mouse Anti-CCNE2 Recombinant Antibody (CBYY-C0563) (CBMAB-C1728-YY)


See all CCNE2 antibodies
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Published Data
CBMAB-C1728-YY-1
Mouse Anti-CCNE2 Recombinant Antibody (CBYY-C0563) (CBMAB-C1728-YY) in IF
The protein levels of CCNE2 was downregulated in NR1H4 knockdown cells by immunofluorescence. ...View More
Huang, S., Hou, Y., Hu, M., Hu, J., & Liu, X. (2022). Clinical significance and oncogenic function of NR1H4 in clear cell renal cell carcinoma. BMC cancer, 22(1), 995.
CBMAB-C1728-YY-2
Mouse Anti-CCNE2 Recombinant Antibody (CBYY-C0563) (CBMAB-C1728-YY) in WB
Western blot revealed that knockdown of NR1H4 inhibited CDK2 mRNA and protein level in 786-O cells. ...View More
Huang, S., Hou, Y., Hu, M., Hu, J., & Liu, X. (2022). Clinical significance and oncogenic function of NR1H4 in clear cell renal cell carcinoma. BMC cancer, 22(1), 995.
CBMAB-C1728-YY-3
Mouse Anti-CCNE2 Recombinant Antibody (CBYY-C0563) (CBMAB-C1728-YY) in WB
The cells were treated with 10 μM LPA in the presence or absence of 10 μM ki16425 for 6 h. Cyclin A2, D1, and E2, CDK2, CDK4, and p27Kip1 protein levels were analyzed by Western blotting, quantified using ImageJ software, and normalized to those of β-actin. ...View More
Kim, D., Kim, H. J., Baek, J. O., Roh, J. Y., & Jun, H. S. (2021). Lysophosphatidic acid mediates imiquimod-induced psoriasis-like symptoms by promoting keratinocyte proliferation through LPAR1/ROCK2/PI3K/AKT signaling pathway. International Journal of Molecular Sciences, 22(19), 10777.

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
CBYY-C0563
Antibody Isotype
IgG2a, κ
Application
WB, IP, IF, ELISA

Basic Information

Immunogen
Amino acids 1-140 of cyclin E2 of human origin.
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
ELISA1:100-1:1,000
IF(ICC)1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Cyclin E2
Entrez Gene ID
9134
UniProt ID
O96020
Alternative Names
Cholinergic Receptor Nicotinic Delta Subunit; Acetylcholine Receptor, Nicotinic, Delta (Muscle); Cholinergic Receptor, Nicotinic, Delta (Muscle); Cholinergic Receptor, Nicotinic Delta; ACHRD; Cholinergic Receptor, Nicotinic, Delta Polypeptide; Cholinergic Receptor, Nicotinic, Delta; Acetylcholine Receptor Subunit Delta;
Function
Essential for the control of the cell cycle at the late G1 and early S phase.
Biological Process
Cell division Source: UniProtKB-KW
DNA replication initiation Source: Ensembl
G1/S transition of mitotic cell cycle Source: GO_Central
Homologous chromosome pairing at meiosis Source: Ensembl
Mitotic cell cycle phase transition Source: GO_Central
Regulation of cellular protein localization Source: Ensembl
Regulation of cyclin-dependent protein serine/threonine kinase activity Source: GO_Central
Telomere maintenance Source: Ensembl
Cellular Location
Nucleus
PTM
Phosphorylation by CDK2 triggers its release from CDK2 and degradation via the ubiquitin proteasome pathway.
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For research use only. Not intended for any clinical use.

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