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Rabbit Anti-CCND3 (Phospho-Thr283) Recombinant Antibody (CBFYC-1042) (CBMAB-C1099-FY)


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Summary

Host Animal
Rabbit
Specificity
Human
Clone
CBFYC-1042
Antibody Isotype
IgG
Application
WB

Basic Information

Immunogen
Synthetic phospho-peptide corresponding to residues surrounding Thr283 of human cyclin D3 protein.
Host Species
Rabbit
Specificity
Human
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
HEPES, pH 7.5, 100 µg/ml BSA, 50% glycerol
Preservative
0.02% sodium azide
Concentration
Batch dependent
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Cyclin D3
Introduction
Amplification and overexpression of the CCND3 gene was detected in the glioblastoma cell line CCF‐STTG1, as well as in one primary glioblastoma and in the sarcomatous component of one gliosarcoma.
Entrez Gene ID
Human896
Mouse12445
Rat25193
UniProt ID
HumanP30281
MouseP30282
RatP48961
Alternative Names
Cyclin D3
Function
Regulatory component of the cyclin D3-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G1/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G1 phase. Hypophosphorylates RB1 in early G1 phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D3/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex.
Biological Process
Cell division Source: UniProtKB-KW
Mitotic cell cycle phase transition Source: GO_Central
Negative regulation of transcription by RNA polymerase II Source: Ensembl
Positive regulation of cyclin-dependent protein serine/threonine kinase activity Source: BHF-UCL
Positive regulation of G1/S transition of mitotic cell cycle Source: GO_Central
Positive regulation of protein phosphorylation Source: BHF-UCL
Regulation of cell population proliferation Source: Ensembl
Regulation of cyclin-dependent protein serine/threonine kinase activity Source: GO_Central
Regulation of insulin receptor signaling pathway Source: Ensembl
Signal transduction Source: Ensembl
T cell proliferation Source: Ensembl
Cellular Location
Cytoplasm; Nucleus; Membrane. Cyclin D-CDK4 complexes accumulate at the nuclear membrane and are then translocated to the nucleus through interaction with KIP/CIP family members.
PTM
Polyubiquitinated by the SCF(FBXL2) complex, leading to proteasomal degradation.
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For research use only. Not intended for any clinical use.

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