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Mouse Anti-CCNB2 Recombinant Antibody (CBFYC-1024) (CBMAB-C1081-FY)


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Published Data
CBMAB-C1081-FY-1
Mouse Anti-CCNB2 Recombinant Antibody (CBFYC-1024) (CBMAB-C1081-FY) in WB
Western blot analyses of E2F1, CDK1, CDK4, and Cyclin B2 expressions in U87MG cells. ...View More
Wang, R., Li, X., Sun, C., Yu, L., Hua, D., Shi, C., ... & Yu, S. (2021). The ATPase Pontin is a key cell cycle regulator by amplifying E2F1 transcription response in glioma. Cell death & disease, 12(2), 141.
CBMAB-C1081-FY-3
Mouse Anti-CCNB2 Recombinant Antibody (CBFYC-1024) (CBMAB-C1081-FY) in WB
Expression pattern of cell cycle regulating target proteins after TAPIR-1 knockdown. Post siRNA transfection protein lysates were prepared at 48 h and 72 h. SDS-PAGE and western blot were performed to detect upregulation of p53 and p21CIP1 and downregulation of CDC25C, cyclin B2/CCNB2, KIF23, MYBL2, and Survivin/BIRC-5 protein targets. β-actin was used as loading control. One of two independent experiments with equal results is shown. ...View More
Friedrich, M., Wiedemann, K., Reiche, K., Puppel, S. H., Pfeifer, G., Zipfel, I., ... & Horn, F. (2020). The role of lncRNAs TAPIR-1 and-2 as diagnostic markers and potential therapeutic targets in prostate cancer. Cancers, 12(5), 1122.
CBMAB-C1081-FY-4
Mouse Anti-CCNB2 Recombinant Antibody (CBFYC-1024) (CBMAB-C1081-FY) in IHC
Immunohistochemistry of BCL11B (blue) and SATB2 (red) in E18.5 cortices electroporated with CYCLIN-B2, CYCLIN-D2 or GFP (green) at E12.5. Quantifications show the percentage of GFP+ cells expressing BCL11B or SATB2 (n=4 biological independent experiments). Pvalues, CYCLIN-B2 electroporation vs. GFP; BCL11B 3.05e-4, SATB2 9.67e-7. N=3 biologically independent experiments. ...View More
Hagey, D. W., Topcic, D., Kee, N., Reynaud, F., Bergsland, M., Perlmann, T., & Muhr, J. (2020). CYCLIN-B1/2 and-D1 act in opposition to coordinate cortical progenitor self-renewal and lineage commitment. Nature communications, 11(1), 2898.
CBMAB-C1081-FY-5
Mouse Anti-CCNB2 Recombinant Antibody (CBFYC-1024) (CBMAB-C1081-FY) in Immunoblot
Levels of mitotic cyclins in G2- and M-arrested cells analyzed by immuno-blotting with indicated antibodies. Each panel shows four steps of a serial 1:1 dilution of extracts. ...View More
Rata, S., Rodriguez, M. F. S. P., Joseph, S., Peter, N., Iturra, F. E., Yang, F., ... & Hochegger, H. (2018). Two interlinked bistable switches govern mitotic control in mammalian cells. Current biology, 28(23), 3824-3832.

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
CBFYC-1024
Antibody Isotype
IgG1, κ
Application
WB, IP, IF, ELISA

Basic Information

Immunogen
Amino acids 1-105 of cyclin B2 of human origin.
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
ELISA1:100-1:1,000
IF(ICC)1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Cyclin B2
Introduction
CCNB2 (Cyclin B2) is a Protein Coding gene. Diseases associated with CCNB2 include Anauxetic Dysplasia 1 and Breast Cancer. Among its related pathways are Mitotic Prometaphase and Mitotic Prophase. Gene Ontology (GO) annotations related to this gene include protein kinase binding. An important paralog of this gene is CCNB1.
Entrez Gene ID
9133
UniProt ID
O95067
Alternative Names
Cyclin B2; G2/Mitotic-Specific Cyclin-B2; HsT17299
Function
Essential for the control of the cell cycle at the G2/M (mitosis) transition.
Biological Process
Cell division Source: UniProtKB-KW
G2/MI transition of meiotic cell cycle Source: Ensembl
G2/M transition of mitotic cell cycle Source: Reactome
In utero embryonic development Source: Ensembl
Mitotic cell cycle phase transition Source: GO_Central
Mitotic nuclear envelope disassembly Source: Reactome
Mitotic nuclear envelope reassembly Source: Reactome
Regulation of cell cycle Source: Reactome
Regulation of cyclin-dependent protein serine/threonine kinase activity Source: GO_Central
Regulation of growth Source: Ensembl
Spindle assembly involved in female meiosis I Source: Ensembl
T cell homeostasis Source: Ensembl
Thymus development Source: Ensembl
Cellular Location
Centrosome; Microtubule cytoskeleton; Cytosol; Nucleus; Cyclin-dependent protein kinase holoenzyme complex; Cytoplasm; Membrane
More Infomation

Wu, S., Su, R., & Jia, H. (2021). Cyclin B2 (CCNB2) Stimulates the Proliferation of Triple-Negative Breast Cancer (TNBC) Cells In Vitro and In Vivo. Disease Markers, 2021.

Mamoor, S. (2021). Differential expression of cyclin B2 in human epithelial ovarian cancer.

Yang, S. Y., Ren, H., Li, C. F., & Tang, H. (2020). Screening core genes and cyclin B2 as a potential diagnosis, treatment and prognostic biomarker of hepatocellular carcinoma based on bioinformatics analysis. Zhonghua gan Zang Bing za zhi= Zhonghua Ganzangbing Zazhi= Chinese Journal of Hepatology, 28(9), 773-783.

Li, R., Jiang, X., Zhang, Y., Wang, S., Chen, X., Yu, X., ... & Huang, X. (2019). Cyclin B2 overexpression in human hepatocellular carcinoma is associated with poor prognosis. Archives of medical research, 50(1), 10-17.

Li, H., Tian, X., Wang, P., Huang, M., Xu, R., & Nie, T. (2019). MicroRNA-582–3p negatively regulates cell proliferation and cell cycle progression in acute myeloid leukemia by targeting cyclin B2. Cellular & molecular biology letters, 24(1), 1-12.

Daldello, E. M., Luong, X. G., Yang, C. R., Kuhn, J., & Conti, M. (2019). Cyclin B2 is required for progression through meiosis in mouse oocytes. Development, 146(8), dev172734.

Li, J., Tang, J. X., Cheng, J. M., Hu, B., Wang, Y. Q., Aalia, B., ... & Liu, Y. X. (2018). Cyclin B2 can compensate for Cyclin B1 in oocyte meiosis I. Journal of Cell Biology, 217(11), 3901-3911.

Gao, C. L., Wang, G. W., Yang, G. Q., Yang, H., & Zhuang, L. (2018). Karyopherin subunit-α 2 expression accelerates cell cycle progression by upregulating CCNB2 and CDK1 in hepatocellular carcinoma. Oncology letters, 15(3), 2815-2820.

Han, S. J., Martins, J. P. S., Yang, Y., Kang, M. K., Daldello, E. M., & Conti, M. (2017). The translation of cyclin B1 and B2 is differentially regulated during mouse oocyte reentry into the meiotic cell cycle. Scientific reports, 7(1), 1-12.

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For research use only. Not intended for any clinical use.

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