Mouse Anti-CCDC50 Recombinant Antibody (CBLY2-115) (CBMAB-Y0042-LY)

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Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

CBLY2-115

Antibody Isotype

IgG2a, κ

Application

WB, IP, IF, ELISA

Basic Information

Immunogen

Amino acids 1-100 mapping at the N-terminus of YMER of human origin.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
ELISA	1:100-1:1,000
IF(ICC)	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name

coiled-coil domain containing 50

Introduction

This gene encodes a soluble, cytoplasmic, tyrosine-phosphorylated protein with multiple ubiquitin-interacting domains. Mutations in this gene cause nonsyndromic, postlingual, progressive sensorineural DFNA44 hearing loss. In mouse, the protein is expressed in the inner ear during development and postnatal maturation and associates with microtubule-based structures. Involved in EGFR signaling.

Entrez Gene ID

152137

UniProt ID

Q8IVM0

Alternative Names

YMER; C3orf6; DFNA44

Function

Involved in EGFR signaling.

Biological Process

Sensory perception of sound Source: MGI

Cellular Location

Cytoplasm. Associated with microtubules of the cytoskeleton and mitotic apparatus.

Involvement in disease

Deafness, autosomal dominant, 44 (DFNA44): A form of non-syndromic deafness characterized by initially moderate hearing loss that affects mainly low to mid frequencies. Later, it progresses to involve all the frequencies and leads to a profound hearing loss by the 6th decade.

PTM

Phosphorylated on tyrosine residues.

More Infomation

References

Hou, P., Yang, K., Jia, P., Liu, L., Lin, Y., Li, Z., ... & Guo, D. (2021). A novel selective autophagy receptor, CCDC50, delivers K63 polyubiquitination-activated RIG-I/MDA5 for degradation during viral infection. Cell research, 31(1), 62-79.

Hou, P., Lin, Y., Li, Z., Lu, R., Wang, Y., Tian, T., ... & Guo, D. (2021). Autophagy receptor CCDC50 tunes the STING-mediated interferon response in viral infections and autoimmune diseases. Cellular & molecular immunology, 18(10), 2358-2371.

Min, J. S., Halder, D., Yoon, J. Y., Jeon, S. J., Jun, S. Y., Lee, J. R., ... & Kim, N. S. (2020). Coiled-coil domain containing 50-V2 protein positively regulates neurite outgrowth. Scientific reports, 10(1), 1-11.

Sun, G., Zhou, H., Chen, K., Zeng, J., Zhang, Y., Yan, L., ... & Xu, H. (2020). HnRNP A1-mediated alternative splicing of CCDC50 contributes to cancer progression of clear cell renal cell carcinoma via ZNF395. Journal of Experimental & Clinical Cancer Research, 39(1), 1-16.

Wang, H., Zhang, C. Z., Lu, S. X., Zhang, M. F., Liu, L. L., Luo, R. Z., ... & Yun, J. P. (2019). A coiled‐coil domain containing 50 splice variant is modulated by serine/arginine‐rich splicing factor 3 and promotes hepatocellular carcinoma in mice by the Ras signaling pathway. Hepatology, 69(1), 179-195.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Associated Products

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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Documents

Datasheet
SDS
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Second antibody and isotype control

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