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Mouse Anti-CASP9 Recombinant Antibody (BA0430) (CBMAB-0168CQ)


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Published Data
CBMAB-0168CQ-1
Mouse Anti-CASP9 Recombinant Antibody (BA0430) (CBMAB-0168CQ) in Immunoblot
Representative immunoblot showing procaspase-8, procaspase-9, BAX, and caspase-3 expression in R4 and R2 cells untreated or exposed to 4 μM MK-8776 for 96 h and 48 h, respectively. The anti-caspase-3 antibody allowed the detection of both the pro-caspase and the active caspase. ...View More
Franza, M., Albanesi, J., Mancini, B., Pennisi, R., Leone, S., Acconcia, F., ... & di Masi, A. (2023). The clinically relevant CHK1 inhibitor MK-8776 induces the degradation of the oncogenic protein PML-RARα and overcomes ATRA resistance in acute promyelocytic leukemia cells. Biochemical Pharmacology, 214, 115675.
CBMAB-0168CQ-2
Mouse Anti-CASP9 Recombinant Antibody (BA0430) (CBMAB-0168CQ) in WB
Analysis of the effect of oral BS administration on apoptosis: Western blot analysis of: Bcl-2 (A), Bax (B), Pro caspase 9 (C), cleaved caspase 9 (D). For the analyses, n = 5 animals from each group were employed. A p-value of less than 0.05 was considered significant. * p < 0.05 vs. control, ** p < 0.01 vs. control. ...View More
D'Amico, R., Impellizzeri, D., Cordaro, M., Siracusa, R., Interdonato, L., Crupi, R., ... & Di Paola, R. (2022). Regulation of apoptosis and oxidative stress by oral boswellia serrata gum resin extract in a rat model of endometriosis. International Journal of Molecular Sciences, 23(23), 15348.
CBMAB-0168CQ-3
Mouse Anti-CASP9 Recombinant Antibody (BA0430) (CBMAB-0168CQ) in WB
Western blotting was conducted specifically for cleaved caspase-3, cleaved caspase-7 and cleaved caspase-9. ...View More
Chen, C. H., Hsu, P. C., Hsu, S. W., Hong, K. T., Chen, K. Y., He, J. L., ... & Tsai, C. W. (2022). Protective effects of jujubosides on 6-OHDA-induced neurotoxicity in SH-SY5Y and SK-N-SH cells. Molecules, 27(13), 4106.

Summary

Host Animal
Mouse
Specificity
Human
Clone
BA0430
Antibody Isotype
IgG1, κ
Application
IP, WB

Basic Information

Immunogen
Fragment of caspase-9 of human origin.
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Purity
>95% as determined by analysis by SDS-PAGE
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Caspase 9
Introduction
This gene encodes a member of the cysteine-aspartic acid protease (caspase) family.Caspases (cysteinyl aspartate proteases) are involved in the signaling pathways of apoptosis, necrosis and inflammation. These enzymes can be divided into initiators and effectors. The initiator isoforms are activated by, and interact with, upstream adaptor molecules.
Entrez Gene ID
Human842
Rat58918
UniProt ID
HumanP55211
RatQ9JHK1
Alternative Names
CASP9; APAF-3; APAF3; ICE-LAP6; MCH6; PPP1R56; caspase-9
Function
Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Promotes DNA damage-induced apoptosis in a ABL1/c-Abl-dependent manner. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP).
Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9.
Biological Process
Activation of cysteine-type endopeptidase activity involved in apoptotic process Source: GO_Central
Activation of cysteine-type endopeptidase activity involved in apoptotic process by cytochrome c Source: Reactome
Apoptotic process Source: UniProtKB
Cellular response to DNA damage stimulus Source: UniProtKB
Cellular response to UV Source: UniProtKB
Extrinsic apoptotic signaling pathway in absence of ligand Source: Reactome
Intrinsic apoptotic signaling pathway in response to DNA damage Source: UniProtKB
Platelet formation Source: UniProtKB
Positive regulation of apoptotic process Source: UniProtKB
Regulation of response to DNA damage stimulus Source: UniProtKB
Signal transduction in response to DNA damage Source: UniProtKB
Cellular Location
Apoptosome; Cytosol; Cytoplasm; Protein-containing complex
PTM
Cleavages at Asp-315 by granzyme B and at Asp-330 by caspase-3 generate the two active subunits. Caspase-8 and -10 can also be involved in these processing events.
Phosphorylated at Thr-125 by MAPK1/ERK2. Phosphorylation at Thr-125 is sufficient to block caspase-9 processing and subsequent caspase-3 activation. Phosphorylation on Tyr-153 by ABL1/c-Abl; occurs in the response of cells to DNA damage.
More Infomation

Han, J., Goldstein, L. A., Hou, W., Watkins, S. C., & Rabinowich, H. (2021). Involvement of CASP9 (caspase 9) in IGF2R/CI-MPR endosomal transport. Autophagy, 17(6), 1393-1409.

Doke, T., Huang, S., Qiu, C., Sheng, X., Seasock, M., Liu, H., ... & Susztak, K. (2021). Genome-wide association studies identify the role of caspase-9 in kidney disease. Science advances, 7(45), eabi8051.

Liao, G., Wang, R., & Tang, D. D. (2021). Plk1 Regulates Caspase-9 Phosphorylation at Ser-196 and Apoptosis of Human Airway Smooth Muscle Cells. American journal of respiratory cell and molecular biology, (ja).

Avrutsky, M. I., & Troy, C. M. (2021). Caspase-9: A Multimodal Therapeutic Target With Diverse Cellular Expression in Human Disease. Frontiers in Pharmacology, 12, 1728.

An, H. K., Chung, K. M., Park, H., Hong, J., Gim, J. E., Choi, H., ... & Yu, S. W. (2020). CASP9 (caspase 9) is essential for autophagosome maturation through regulation of mitochondrial homeostasis. Autophagy, 16(9), 1598-1617.

Han, C., Liu, Z., Zhang, Y., Shen, A., Dong, C., Zhang, A., ... & Fu, Y. X. (2020). Tumor cells suppress radiation-induced immunity by hijacking caspase 9 signaling. Nature immunology, 21(5), 546-554.

Zhang, L., Zhang, X., Wang, X., He, M., & Qiao, S. (2019). MicroRNA-224 promotes tumorigenesis through downregulation of caspase-9 in triple-negative breast cancer. Disease markers, 2019.

Ercan, S., Arinc, S., Yilmaz, S. G., Altunok, C., Yaman, F., & Isbir, T. (2019). Investigation of caspase 9 gene polymorphism in patients with non-small cell lung cancer. Anticancer research, 39(5), 2437-2441.

Iwai, N., Yasui, K., Tomie, A., Gen, Y., Terasaki, K., Kitaichi, T., ... & Itoh, Y. (2018). Oncogenic miR-96-5p inhibits apoptosis by targeting the caspase-9 gene in hepatocellular carcinoma. International journal of oncology, 53(1), 237-245.

Lu, J., Peng, J., Xiang, M., He, L., Wang, D., Xiong, G., & Li, S. (2018). Trichosanthes kirilowii lectin alleviates diabetic nephropathy by inhibiting the LOX1/NF-κB/caspase-9 signaling pathway. Bioscience reports, 38(5), BSR20180071.

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For research use only. Not intended for any clinical use.

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