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Mouse Anti-CAP1 Recombinant Antibody (CBLNC-139) (CBMAB-1303-CN)


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Published Data
CBMAB-1303-CN-1
Mouse Anti-CAP1 Recombinant Antibody (CBLNC-139) (CBMAB-1303-CN) in IHC
Immunohistochemical detection of the six proteins differentially expressed in ACC and normal adrenal. Representative images of immunohistochemistry performed on tumor specimens (n=4) and normal adrenals (n=3) revealed marked positivity to CAP-1 in the cytosol of almost all tumor cells in the field. ...View More
Poli, G., Ceni, E., Armignacco, R., Ercolino, T., Canu, L., Baroni, G., ... & Luconi, M. (2015). 2D-DIGE proteomic analysis identifies new potential therapeutic targets for adrenocortical carcinoma. Oncotarget, 6(8), 5695.
CBMAB-1303-CN-2
Mouse Anti-CAP1 Recombinant Antibody (CBLNC-139) (CBMAB-1303-CN) in WB
Western blot analysis of six proteins differentially expressed in ACC and normal adrenal. Differential expression of CAP-1 as detected by a representative Western blot of the same pool of ACC and normal adrenal (NOR) samples used in 2D-DIGE. ...View More
Poli, G., Ceni, E., Armignacco, R., Ercolino, T., Canu, L., Baroni, G., ... & Luconi, M. (2015). 2D-DIGE proteomic analysis identifies new potential therapeutic targets for adrenocortical carcinoma. Oncotarget, 6(8), 5695.

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBLNC-139
Antibody Isotype
IgG2a, κ
Application
ELISA, IF, IP, WB

Basic Information

Immunogen
Amino acids 14-74 mapping near the N-terminus of CAP1 of human origin.
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG2a, κ
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
ELISA1:100-1:1,000
IF(ICC)1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
Localized in aa. 1-475

Target

Full Name
Cyclase Associated Actin Cytoskeleton Regulatory Protein 1
Introduction
The protein encoded by this gene is related to the S. cerevisiae CAP protein, which is involved in the cyclic AMP pathway. The human protein is able to interact with other molecules of the same protein, as well as with CAP2 and actin. It directly regulates filament dynamics and has been implicated in a number of complex developmental and morphological processes, including mRNA localization and the establishment of cell polarity.
Entrez Gene ID
10487
UniProt ID
Q01518
Alternative Names
CAP; CAP1-PEN
Function
Directly regulates filament dynamics and has been implicated in a number of complex developmental and morphological processes, including mRNA localization and the establishment of cell polarity.
Biological Process
Actin cytoskeleton organization Source: Ensembl
Activation of adenylate cyclase activity Source: ProtInc
Ameboidal-type cell migration Source: Ensembl
cAMP-mediated signaling Source: GO_Central
Cell morphogenesis Source: GO_Central
Establishment or maintenance of cell polarity Source: ProtInc
Neutrophil degranulation Source: Reactome
Receptor-mediated endocytosis Source: Ensembl
Regulation of adenylate cyclase activity Source: GO_Central
Signal transduction Source: ProtInc
Cellular Location
Cell membrane
More Infomation

Schneider, F., Duong, T. A., Metz, I., Winkelmeier, J., Hübner, C. A., Endesfelder, U., & Rust, M. B. (2021). Mutual functional dependence of cyclase-associated protein 1 (CAP1) and cofilin1 in neuronal actin dynamics and growth cone function. Progress in Neurobiology, 202, 102050.

Zhang, X., Pizzoni, A., Hong, K., Naim, N., Qi, C., Korkhov, V., & Altschuler, D. L. (2021). CAP1 binds and activates adenylyl cyclase in mammalian cells. Proceedings of the National Academy of Sciences, 118(24).

Zhang, H., Ramsey, A., Xiao, Y., Karki, U., Xie, J. Y., Xu, J., ... & Zhou, G. L. (2020). Dynamic phosphorylation and dephosphorylation of cyclase-associated protein 1 by antagonistic signaling through cyclin-dependent kinase 5 and cAMP are critical for the protein functions in actin filament disassembly and cell adhesion. Molecular and cellular biology, 40(4), e00282-19.

Bergqvist, M., Elebro, K., Sandsveden, M., Borgquist, S., & Rosendahl, A. H. (2020). Effects of tumor-specific CAP1 expression and body constitution on clinical outcomes in patients with early breast cancer. Breast Cancer Research, 22(1), 1-15.

Yuan, X., Zhen, Z., Zhang, M., Yu, Y., Gao, X., & Ao, J. X. (2019). Cyclase‐associated protein 1 is a key negative regulator of milk synthesis and proliferation of bovine mammary epithelial cells. Cell biochemistry and function, 37(3), 185-192.

Hasan, R., & Zhou, G. L. (2019). The cytoskeletal protein cyclase-associated protein 1 (CAP1) in breast cancer: context-dependent roles in both the invasiveness and proliferation of cancer cells and underlying cell signals. International journal of molecular sciences, 20(11), 2653.

Wu, H., Hasan, R., Zhang, H., Gray, J., Williams, D., Miller, M., ... & Zhou, G. L. (2019). Phosphorylation regulates CAP1 (cyclase-associated protein 1) functions in the motility and invasion of pancreatic cancer cells. Scientific reports, 9(1), 1-13.

Kakurina, G. V., Kolegova, E. S., & Kondakova, I. V. (2018). Adenylyl cyclase-associated protein 1: structure, regulation, and participation in cellular processes. Biochemistry (Moscow), 83(1), 45-53.

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For research use only. Not intended for any clinical use.

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