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Mouse Anti-CAND1 Recombinant Antibody (CBLC078-LY) (CBMAB-C9679-LY)


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Published Data
CBMAB-C9679-LY-1
Mouse Anti-CAND1 Recombinant Antibody (CBLC078-LY) (CBMAB-C9679-LY) in IF
Restoration of ER morphology in the CAND1 knocked-down cells by exogenous Lnp expression. COS-7 cells were transfected with siCAND1 #2, followed by transfection with mCherry or mCherry-tagged Lnp (Lnp-mChe). The cells were immunostained with the anti-CLIMP-63 mAb. The ratios of CLIMP-63-staining area to total cell area are shown in the rightmost panel, in which each dot indicates the ratio in a single cell. N = 15. The averages ± SD are indicated by red lines. ***P < 0.001; paired Student's t-test. Scale bars, 10 μm. ...View More
Kajiho, H., Yamamoto, Y., & Sakisaka, T. (2019). CAND1 regulates lunapark for the proper tubular network of the endoplasmic reticulum. Scientific Reports, 9(1), 13152.
CBMAB-C9679-LY-2
Mouse Anti-CAND1 Recombinant Antibody (CBLC078-LY) (CBMAB-C9679-LY) in Immunoblot
Decrease of the protein level of endogenous Lnp in the CAND1 knocked-down cells. COS-7 cells were transfected with the control siRNA or three independent siRNAs targeting CAND1 (siCAND1 #1-3). The total cell extracts were subjected to SDS-PAGE followed by immunoblotting with the anti-CAND1 mAb, the anti-Lnp pAb, and the anti-actin mAb. The intensities of immunoreactive bands for Lnp were normalized to those for actin and expressed as percentages of the control siRNA-transfected cells in the right panel. Data are the averages ± SEM of three independent experiments. *P < 0.05, ***P < 0.001; paired Student's t-test. ...View More
Kajiho, H., Yamamoto, Y., & Sakisaka, T. (2019). CAND1 regulates lunapark for the proper tubular network of the endoplasmic reticulum. Scientific Reports, 9(1), 13152.
CBMAB-C9679-LY-3
Mouse Anti-CAND1 Recombinant Antibody (CBLC078-LY) (CBMAB-C9679-LY) in IP
Identification of CAND1 and SND1 as PI(4,5)P2 binding cellular proteins. Extracts from KGMN/TERT-pLXSN and KGMN/TERT-8E6 were incubated with A/G-agarose coupled with either CAND1, SND1 or normal IgG-mouse antibodies. Co-immunopecipitated PI(4,5)P2 and 10% of the input extracts were detected by Western blot with specific antibodies against PI(4,5)P2. Total levels of PI(4,5)P2, CAND1 and SND1 were detected using specific antibodies. Equal protein loading was confirmed by immunoblotting for tubulin. ...View More
Marx, B., Hufbauer, M., Zigrino, P., Majewski, S., Markiefka, B., Sachsenheimer, T., ... & Akgül, B. (2018). Phospholipidation of nuclear proteins by the human papillomavirus E6 oncoprotein: implication in carcinogenesis. Oncotarget, 9(75), 34142.

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
CBLC078-LY
Antibody Isotype
IgG1, κ
Application
ELISA, IF, IP, WB, IHC-P

Basic Information

Immunogen
Amino acids 396-480 of TIP120A of human origin.
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
ELISA1:100-1:1,000
IF(ICC)1:50-1:500
IHC-P1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Cullin Associated And Neddylation Dissociated 1
Introduction
CAND1 (Cullin Associated And Neddylation Dissociated 1) is a Protein Coding gene. Diseases associated with CAND1 include Familial Hypertension. Among its related pathways are Innate Immune System and Mesodermal Commitment Pathway. Gene Ontology (GO) annotations related to this gene include binding and TBP-class protein binding. An important paralog of this gene is CAND2.
Entrez Gene ID
55832
UniProt ID
Q86VP6
Alternative Names
Cullin Associated And Neddylation Dissociated 1; TBP-Interacting Protein Of 120 KDa A; P120 CAND1; TIP120A; TIP120; Cullin-Associated And Neddylation-Dissociated Protein 1;
Function
Key assembly factor of SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complexes that promotes the exchange of the substrate-recognition F-box subunit in SCF complexes, thereby playing a key role in the cellular repertoire of SCF complexes. Acts as a F-box protein exchange factor. The exchange activity of CAND1 is coupled with cycles of neddylation conjugation: in the deneddylated state, cullin-binding CAND1 binds CUL1-RBX1, increasing dissociation of the SCF complex and promoting exchange of the F-box protein. Probably plays a similar role in other cullin-RING E3 ubiquitin ligase complexes.
Biological Process
Cell differentiation Source: UniProtKB
Cellular iron ion homeostasis Source: Reactome
Negative regulation of catalytic activity Source: UniProtKB
Neutrophil degranulation Source: Reactome
Positive regulation of RNA polymerase II transcription preinitiation complex assembly Source: Ensembl
Post-translational protein modification Source: Reactome
Protein ubiquitination Source: UniProtKB
SCF complex assembly Source: UniProtKB
Cellular Location
Nucleus; Cytoplasm. Predominantly cytoplasmic.
More Infomation

Pan, Z., Li, X., Zhang, Y., Zhao, Y., Zhou, Y., Han, Q., ... & Yang, B. (2021). Cullin-associated and neddylation-dissociated 1 protein (CAND1) governs cardiac hypertrophy and heart failure partially through regulating calcineurin degradation.

Cheng, W., Yin, S., Tu, Y., Mei, H., Wang, Y., & Yang, Y. (2020). SlCAND1, encoding cullin-associated Nedd8-dissociated protein 1, regulates plant height, flowering time, seed germination, and root architecture in tomato. Plant molecular biology, 102(4), 537-551.

Kajiho, H., Yamamoto, Y., & Sakisaka, T. (2019). CAND1 regulates lunapark for the proper tubular network of the endoplasmic reticulum. Scientific reports, 9(1), 1-16.

Che, Z., Liu, F., Zhang, W., McGrath, M., Hou, D., Chen, P., ... & Yang, D. (2018). Targeting CAND1 promotes caspase-8/RIP1-dependent apoptosis in liver cancer cells. American journal of translational research, 10(5), 1357.

Nakashima, K., Takeuchi, S., Iwama, S., Kiyota, A., Yasuda, Y., Iwata, N., ... & Sugimura, Y. (2018). Cullin-associated NEDD8-dissociated protein 1, a novel interactor of rabphilin-3A, deubiquitylates rabphilin-3A and regulates arginine vasopressin secretion in PC12 cells. endocrine journal, EJ17-0399.

Buonanno, M., Langella, E., Zambrano, N., Succoio, M., Sasso, E., Alterio, V., ... & De Simone, G. (2017). Disclosing the interaction of carbonic anhydrase IX with cullin-associated NEDD8-dissociated protein 1 by molecular modeling and integrated binding measurements. ACS chemical biology, 12(6), 1460-1465.

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For research use only. Not intended for any clinical use.

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