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Rabbit Anti-CAMK4 Recombinant Antibody (BA0042) (CBMAB-0150CQ)


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Summary

Host Animal
Rabbit
Specificity
Human, Mouse, Rat
Clone
BA0042
Antibody Isotype
IgG
Application
ICC, WB, IHC, ELISA

Basic Information

Immunogen
A synthetic peptide corresponding to a sequence within amino acids 374-473 of human CAMKIV.
Host Species
Rabbit
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:500-1:1,000
IF(ICC)1:50-1:200
IHC-P1:500-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH7.3, 50% glycerol, 0.05% BSA
Preservative
0.05% sodium azide
Concentration
Batch dependent
Purity
>95% as determined by analysis by SDS-PAGE
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Calcium/Calmodulin Dependent Protein Kinase IV
Introduction
The product of this gene belongs to the serine/threonine protein kinase family, and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. Calcium/calmodulin-dependent protein kinase that operates in the calcium-triggered CaMKK-CaMK4 signaling cascade and regulates, mainly by phosphorylation, the activity of several transcription activators, such as CREB1, MEF2D, JUN and RORA, which play pivotal roles in immune response, inflammation, and memory consolidation. In the thymus, regulates the CD4(+)/CD8(+) double positive thymocytes selection threshold during T-cell ontogeny. In CD4 memory T-cells, is required to link T-cell antigen receptor (TCR) signaling to the production of IL2, IFNG and IL4 (through the regulation of CREB and MEF2). Regulates the differentiation and survival phases of osteoclasts and dendritic cells (DCs). Mediates DCs survival by linking TLR4 and the regulation of temporal expression of BCL2. Phosphorylates the transcription activator CREB1 on Ser-133 in hippocampal neuron nuclei and contribute to memory consolidation and long term potentiation (LTP) in the hippocampus. Can activate the MAP kinases MAPK1/ERK2, MAPK8/JNK1 and MAPK14/p38 and stimulate transcription through the phosphorylation of ELK1 and ATF2. Can also phosphorylate in vitro CREBBP, PRM2, MEF2A and STMN1/OP18.
Entrez Gene ID
Human814
Mouse12326
Rat25050
UniProt ID
HumanQ16566
MouseP08414
RatP13234
Alternative Names
Calcium/Calmodulin-Dependent Protein Kinase IV; Calcium/Calmodulin-Dependent Protein Kinase Type IV Catalytic Chain; Brain Ca++-Calmodulin-Dependent Protein Kinase Type IV; CAM Kinase- GR; CAM Kinase IV; CaM Kinase-GR; CaMK IV; CaMK-GR; CaMK; Brain Ca(2+)-Calmodulin-Dependent Protein Kinase Type IV; EC 2.7.11.17; EC 2.7.11; CAMKIV; IV
Function
Calcium/calmodulin-dependent protein kinase that operates in the calcium-triggered CaMKK-CaMK4 signaling cascade and regulates, mainly by phosphorylation, the activity of several transcription activators, such as CREB1, MEF2D, JUN and RORA, which play pivotal roles in immune response, inflammation, and memory consolidation. In the thymus, regulates the CD4+/CD8+ double positive thymocytes selection threshold during T-cell ontogeny. In CD4 memory T-cells, is required to link T-cell antigen receptor (TCR) signaling to the production of IL2, IFNG and IL4 (through the regulation of CREB and MEF2). Regulates the differentiation and survival phases of osteoclasts and dendritic cells (DCs). Mediates DCs survival by linking TLR4 and the regulation of temporal expression of BCL2. Phosphorylates the transcription activator CREB1 on 'Ser-133' in hippocampal neuron nuclei and contribute to memory consolidation and long term potentiation (LTP) in the hippocampus. Can activate the MAP kinases MAPK1/ERK2, MAPK8/JNK1 and MAPK14/p38 and stimulate transcription through the phosphorylation of ELK1 and ATF2. Can also phosphorylate in vitro CREBBP, PRM2, MEF2A and STMN1/OP18.
Biological Process
Adaptive immune response Source: UniProtKB-KW
Chemical synaptic transmission Source: Reactome
Inflammatory response Source: UniProtKB-KW
Intracellular signal transduction Source: GO_Central
Long-term memory Source: UniProtKB
Myeloid dendritic cell differentiation Source: UniProtKB
Peptidyl-serine phosphorylation Source: GO_Central
Positive regulation of transcription, DNA-templated Source: UniProtKB
Protein autophosphorylation Source: GO_Central
Protein phosphorylation Source: ProtInc
Regulation of osteoclast differentiation Source: UniProtKB
Regulation of T cell differentiation in thymus Source: UniProtKB
Signal transduction Source: ProtInc
Cellular Location
Nucleus; Cytoplasm. Localized in hippocampal neuron nuclei. In spermatids, associated with chromatin and nuclear matrix (By similarity).
PTM
Phosphorylated by CaMKK1 and CaMKK2 on Thr-200. Dephosphorylated by protein phosphatase 2A. Autophosphorylated on Ser-12 and Ser-13.
Glycosylation at Ser-189 modulates the phosphorylation of CaMK4 at Thr-200 and negatively regulates its activity toward CREB1 in basal conditions and during early inomycin stimulation.
More Infomation

Alsharif, M. A., Khan, D., Ahmed, N., Mukhtar, S., Khan, P., Hassan, M. I., ... & Obaid, R. J. (2020). Pharmacological Activities of Novel Chromene Derivatives as Calcium/Calmodulin Dependent Protein Kinase IV (CAMKIV) Inhibitors. ChemistrySelect, (2), 498-505.

Naz, H., Tarique, M., Suhail, M., Shankar, H., Muhammad, N., Usmani, D., ... & Zughaibi, T. A. (2020). Calcium-/Calmodulin-Dependent Protein Kinase IV (CAMKIV): A Multifunctional Enzyme and Its Role in Various Cancer: An Update. Current Molecular Biology Reports, 1-9.

Liu, J., Yang, R., Meng, H., Zhou, T., & He, Q. (2020). In vitro treatment of 3 T3-L1 adipocytes with recombinant Calcium/calmodulin-dependent Protein Kinase IV (CaMKIV) limits ER stress and improves insulin sensitivity through inhibition of autophagy via the mTOR/CREB signaling pathway. BMC Endocrine Disorders, 20(1), 1-11.

Gupta, P., Khan, S., Fakhar, Z., Hussain, A., Rehman, M., AlAjmi, M. F., ... & Hassan, M. (2020). Identification of potential inhibitors of calcium/calmodulin-dependent protein kinase IV from bioactive phytoconstituents. Oxidative medicine and cellular longevity, 2020.

Li, Z., Lu, J., Zeng, G., Pang, J., Zheng, X., Feng, J., & Zhang, J. (2019). MiR-129-5p inhibits liver cancer growth by targeting calcium calmodulin-dependent protein kinase IV (CAMK4). Cell death & disease, 10(11), 1-14.

Cunningham, K. E., Novak, E. A., Vincent, G., Siow, V. S., Griffith, B. D., Ranganathan, S., ... & Mollen, K. P. (2019). Calcium/calmodulin–dependent protein kinase IV (CaMKIV) activation contributes to the pathogenesis of experimental colitis via inhibition of intestinal epithelial cell proliferation. The FASEB Journal, 33(1), 1330-1346.

Liu, Q., Wang, L., Yan, G., Zhang, W., Huan, Z., & Li, J. (2019). miR-125a-5p alleviates dysfunction and inflammation of pentylenetetrazol-induced epilepsy through targeting calmodulin-dependent protein kinase IV (CAMK4). Current neurovascular research, 16(4), 365-372.

Beg, A., Khan, F. I., Lobb, K. A., Islam, A., Ahmad, F., & Hassan, M. I. (2019). High throughput screening, docking, and molecular dynamics studies to identify potential inhibitors of human calcium/calmodulin-dependent protein kinase IV. Journal of Biomolecular Structure and Dynamics, 37(8), 2179-2192.

Shi, D., Gu, R., Song, Y., Ding, M., Huang, T., Guo, M., ... & Liao, H. (2018). Calcium/Calmodulin-dependent protein kinase IV (CaMKIV) mediates acute skeletal muscle inflammatory response. Inflammation, 41(1), 199-212.

Ye, S., Wang, T. T., Cai, B., Wang, Y., Li, J., Zhan, J. X., & Shen, G. M. (2017). Genistein protects hippocampal neurons against injury by regulating calcium/calmodulin dependent protein kinase IV protein levels in Alzheimer's disease model rats. Neural regeneration research, 12(9), 1479.

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For research use only. Not intended for any clinical use.

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