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Mouse Anti-CAMK2A Recombinant Antibody (CBXC-1564) (CBMAB-C3539-CQ)


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Published Data
CBMAB-C3539-CQ-1
Mouse Anti-CAMK2A Recombinant Antibody (CBXC-1564) (CBMAB-C3539-CQ) in IF
Representative figure showing the colocalization of c-Fos + and CaMKIIα + cells in the aIC. Scale bar = 20 μm. ...View More
Ma, Y., Jiang, S., Zhao, X., Li, S., Chen, L., Zhao, Z., ... & Wu, H. (2023). CaMKIIα neurons in the anterior insular cortex regulate attention behavior in mice. Frontiers in Neural Circuits, 17, 1197541.
CBMAB-C3539-CQ-2
Mouse Anti-CAMK2A Recombinant Antibody (CBXC-1564) (CBMAB-C3539-CQ) in IP
Co-IP of NRF2 in A549 and H1299 cells with or without stable CaMK2A-OE using CaMK2A as bait. ...View More
Wang, S. Q., Chen, J. J., Jiang, Y., Lei, Z. N., Ruan, Y. C., Pan, Y., ... & Xiao, Z. J. (2023). Targeting GSTP1 as therapeutic strategy against lung adenocarcinoma stemness and resistance to tyrosine kinase inhibitors. Advanced Science, 10(7), 2205262.
CBMAB-C3539-CQ-3
Mouse Anti-CAMK2A Recombinant Antibody (CBXC-1564) (CBMAB-C3539-CQ) in WB
Western-blot immunodetection of CaMKII autophosphorylation in Thr286 and of total CaMKII expression obtained in one individual experiment where hippocampal slices were subjected to Schaffer collateral basal or theta-burst (θ) stimulation in the absence and in the presence (PG) of the selective VPAC1 antagonist PG 97-269 (100 nM). Slices were monitored for 50 min after theta-burst stimulation (or equivalent time for controls) before WB analysis. ...View More
Caulino-Rocha, A., Rodrigues, N. C., Ribeiro, J. A., & Cunha-Reis, D. (2022). Endogenous VIP VPAC1 receptor activation modulates hippocampal theta burst induced LTP: transduction pathways and GABAergic mechanisms. Biology, 11(5), 627.

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
CBXC-1564
Antibody Isotype
IgG2a, κ
Application
WB, IP, IF, IHC-P, ELISA

Basic Information

Immunogen
Amino acids 303-478 of CaMKIIα of mouse origin.
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
ELISA1:100-1:1,000
IF(ICC)1:50-1:500
IHC-P1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Calcium/Calmodulin Dependent Protein Kinase II Alpha
Introduction
CAMK2A (Calcium/Calmodulin Dependent Protein Kinase II Alpha) is a Protein Coding gene. Diseases associated with CAMK2A include Mental Retardation, Autosomal Dominant 53 and Alacrima, Achalasia, And Mental Retardation Syndrome. Among its related pathways are RET signaling and Salivary secretion. Gene Ontology (GO) annotations related to this gene include protein homodimerization activity and protein kinase activity. An important paralog of this gene is CAMK2D.
Entrez Gene ID
12322
UniProt ID
P11798
Alternative Names
Calcium/Calmodulin Dependent Protein Kinase II Alpha; Calcium/Calmodulin-Dependent Protein Kinase (CaM Kinase) II Alpha; Calcium/Calmodulin-Dependent Protein Kinase Type II Alpha Chain; Calcium/Calmodulin-Dependent Protein Kinase II Alpha-B Subunit; CaM Kinase II Alpha Subunit; CaM Kinase II Subunit Alpha; CaM-Kinase II Alpha Chain; CaMK-II Alpha Subunit; CaMK-II Subunit Alpha;
Function
Calcium/calmodulin-dependent protein kinase that functions autonomously after Ca2+/calmodulin-binding and autophosphorylation, and is involved in synaptic plasticity, neurotransmitter release and long-term potentiation. Member of the NMDAR signaling complex in excitatory synapses, it regulates NMDAR-dependent potentiation of the AMPAR and therefore excitatory synaptic transmission (By similarity).
Regulates dendritic spine development (PubMed:28130356).
Also regulates the migration of developing neurons (PubMed:29100089).
Phosphorylates the transcription factor FOXO3 to activate its transcriptional activity (PubMed:23805378).
Acts as a negative regulator of 2-arachidonoylglycerol (2-AG)-mediated synaptic signaling via modulation of DAGLA activity (By similarity).
Biological Process
Angiotensin-activated signaling pathway Source: UniProtKB
Calcium ion transport Source: ParkinsonsUK-UCL
Dendritic spine development Source: UniProtKB
G1/S transition of mitotic cell cycle Source: ParkinsonsUK-UCL
Interferon-gamma-mediated signaling pathway Source: Reactome
MAPK cascade Source: Reactome
Negative regulation of hydrolase activity Source: UniProtKB
Peptidyl-serine phosphorylation Source: ParkinsonsUK-UCL
Peptidyl-threonine autophosphorylation Source: UniProtKB
Positive regulation of calcium ion transport Source: ParkinsonsUK-UCL
Positive regulation of cardiac muscle cell apoptotic process Source: ParkinsonsUK-UCL
Positive regulation of NF-kappaB transcription factor activity Source: UniProtKB
Protein autophosphorylation Source: ParkinsonsUK-UCL
Protein phosphorylation Source: UniProtKB
Regulation of cellular response to heat Source: Reactome
Regulation of endocannabinoid signaling pathway Source: UniProtKB
Regulation of mitochondrial membrane permeability involved in apoptotic process Source: ParkinsonsUK-UCL
Regulation of neuronal synaptic plasticity Source: ParkinsonsUK-UCL
Regulation of neuron migration Source: UniProtKB
Regulation of neurotransmitter secretion Source: ParkinsonsUK-UCL
Regulation of NMDA receptor activity Source: Reactome
Response to ischemia Source: ParkinsonsUK-UCL
Wnt signaling pathway, calcium modulating pathway Source: Reactome
Cellular Location
Synapse; Postsynaptic density; Dendritic spine; Dendrite. Postsynaptic lipid rafts.
Involvement in disease
Mental retardation, autosomal dominant 53 (MRD53): A form of mental retardation, a disorder characterized by significantly below average general intellectual functioning associated with impairments in adaptive behavior and manifested during the developmental period.
Mental retardation, autosomal recessive 63 (MRT63): A form of mental retardation, a disorder characterized by significantly below average general intellectual functioning associated with impairments in adaptive behavior and manifested during the developmental period. MRT63 patients manifest global developmental delay, severe intellectual disability, and seizures.
PTM
Autophosphorylation of Thr-286 following activation by Ca2+/calmodulin. Phosphorylation of Thr-286 locks the kinase into an activated state.
Palmitoylated. Probably palmitoylated by ZDHHC3 and ZDHHC7.
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For research use only. Not intended for any clinical use.

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