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Mouse Anti-CALCR Recombinant Antibody (CBLNC-135) (CBMAB-1287-CN)


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Published Data
CBMAB-1287-CN-1
Mouse Anti-CALCR Recombinant Antibody (CBLNC-135) (CBMAB-1287-CN) in FC
Settings of the gates for FACS analysis are shown in the upper part of the image. In the lower part of the image, representative images of PBMC isolation, TRAP analysis, DAPI stained cell sorting and results of the resorption assay are presented. ...View More
Steller, D., Scheibert, A., Sturmheit, T., & Hakim, S. G. (2020). Establishment and validation of an in vitro co-culture model for oral cell lines using human PBMC-derived osteoclasts, osteoblasts, fibroblasts and keratinocytes. Scientific Reports, 10(1), 16861.
CBMAB-1287-CN-2
Mouse Anti-CALCR Recombinant Antibody (CBLNC-135) (CBMAB-1287-CN) in IHC
Effects of AA and DHA on actin ring formation and CTR expression.
Osteoclast differentiation was stimulated in CD14+ monocytes by the addition of 25 ng ml-1 M-CSF and 30 ng ml-1 RANKL as described in Materials and Methods. Vehicle (0.08% ethanol) or LCPUFAs (40 μM) were added from day 3 in differentiating osteoclasts and from the onset of resorption (day 12-14) in mature osteoclasts. After 3 weeks of culture, cells were fixed and stained with Hoechst (blue) for nuclei, phalloidin (red) for actin rings, and anti-CTR antibody (green). The results are representative of three independent experiments conducted in triplicate. Scale bar = 200 μm. Diff Ocl-differentiating osteoclasts. Mat Ocl-mature osteoclasts. ...View More
Kasonga, A. E., Deepak, V., Kruger, M. C., & Coetzee, M. (2015). Arachidonic acid and docosahexaenoic acid suppress osteoclast formation and activity in human CD14+ monocytes, in vitro. PloS one, 10(4), e0125145.

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBLNC-135
Antibody Isotype
IgG2a
Application
FC

Basic Information

Immunogen
HEK293 human embryonic kidney cell line transfected with human Calcitonin R Ala25-Ala474.
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG2a
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
FC0.25 µg/10^6 cells

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Lyophilized
Buffer
PBS, Trehalose
Preservative
None
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Calcitonin Receptor
Introduction
CALCR is a high affinity receptor for the peptide hormone calcitonin and belongs to a subfamily of seven transmembrane-spanning G protein-coupled receptors. CALCR is involved in maintaining calcium homeostasis and in regulating osteoclast-mediated bone resorption and polymorphisms in CALCR have been associated with variations in bone mineral density and onset of osteoporosis.
Entrez Gene ID
799
UniProt ID
P30988
Alternative Names
CRT; CTR; CT-R; CTR1
Function
This is a receptor for calcitonin. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase. The calcitonin receptor is thought to couple to the heterotrimeric guanosine triphosphate-binding protein that is sensitive to cholera toxin.
Isoform 2:
Receptor for calcitonin but is unable to couple to G proteins and activate adenylyl cyclase (PubMed:7476993).
Does not undergo receptor internalization following ligand binding (PubMed:7476993).
Biological Process
Adenylate cyclase-activating G protein-coupled receptor signaling pathway Source: UniProtKB
Adenylate cyclase-modulating G protein-coupled receptor signaling pathway Source: GO_Central
Amylin receptor signaling pathway Source: UniProtKB
Cell surface receptor signaling pathway Source: InterPro
Cross-receptor inhibition within G protein-coupled receptor heterodimer Source: UniProtKB
G protein-coupled receptor signaling pathway Source: Reactome
Negative regulation of inflammatory response to antigenic stimulus Source: Reactome
Positive regulation of adenylate cyclase activity Source: UniProtKB
Positive regulation of calcium ion import across plasma membrane Source: ARUK-UCL
Positive regulation of cell death Source: ARUK-UCL
Positive regulation of cytosolic calcium ion concentration Source: UniProtKB
Positive regulation of ERK1 and ERK2 cascade Source: ARUK-UCL
Positive regulation of gene expression Source: ARUK-UCL
Positive regulation of peptidyl-serine phosphorylation Source: ARUK-UCL
Positive regulation of protein kinase A signaling Source: ARUK-UCL
Positive regulation of protein kinase B signaling Source: ARUK-UCL
Response to amyloid-beta Source: ARUK-UCL
Response to glucocorticoid Source: UniProtKB
Cellular Location
Cell membrane
Topology
Extracellular: 25-152 aa
Helical: 153-173 aa
Cytoplasmic: 174-186 aa
Helical: 187-207 aa
Extracellular: 208-233 aa
Helical: 234-254 aa
Cytoplasmic: 255-258 aa
Helical: 259-279 aa
Extracellular: 280-296 aa
Helical: 297-317 aa
Cytoplasmic: 318-340 aa
Helical: 341-361 aa
Extracellular: 362-373 aa
Helical: 374-394 aa
Cytoplasmic: 395-474 aa
More Infomation

Zhang, L., Kubota, M., Nakamura, A., Kaji, T., Seno, S., Uezumi, A., ... & Fukada, S. I. (2021). Dlk1 regulates quiescence in calcitonin receptor‐mutant muscle stem cells. Stem Cells, 39(3), 306-317.

Gonzalez, I. E., Ramirez-Matias, J., Lu, C., Pan, W., Zhu, A., Myers Jr, M. G., & Olson, D. P. (2021). Paraventricular Calcitonin Receptor–Expressing Neurons Modulate Energy Homeostasis in Male Mice. Endocrinology, 162(6), bqab072.

Yoshihara, C., Tokita, K., Maruyama, T., Kaneko, M., Tsuneoka, Y., Fukumitsu, K., ... & Kuroda, K. O. (2021). Calcitonin receptor signaling in the medial preoptic area enables risk-taking maternal care. Cell Reports, 35(9), 109204.

Cheng, W., Gonzalez, I., Pan, W., Tsang, A. H., Adams, J., Ndoka, E., ... & Myers Jr, M. G. (2020). Calcitonin receptor neurons in the mouse nucleus tractus solitarius control energy balance via the non-aversive suppression of feeding. Cell metabolism, 31(2), 301-312.

Ikemoto‐Uezumi, M., Uezumi, A., Zhang, L., Zhou, H., Hashimoto, N., Okamura, K., ... & Fukada, S. I. (2019). Reduced expression of calcitonin receptor is closely associated with age‐related loss of the muscle stem cell pool. JCSM Rapid Communications, 2(1), 1-13.

Ieda, N., Kawai, N., Ishii, H., Ihara, K., Inoue, N., Uenoyama, Y., & Tsukamura, H. (2018). Co‐expression of the calcitonin receptor gene in the hypothalamic kisspeptin neurons in female rats. Reproductive medicine and biology, 17(2), 164-172.

Pan, W., Adams, J. M., Allison, M. B., Patterson, C., Flak, J. N., Jones, J., ... & Myers Jr, M. G. (2018). Essential Role for Hypothalamic Calcitonin Receptor‒Expressing Neurons in the Control of Food Intake by Leptin. Endocrinology, 159(4), 1860-1872.

Pal, J., Patil, V., Kumar, A., Kaur, K., Sarkar, C., & Somasundaram, K. (2018). Loss-of-function mutations in Calcitonin receptor (CALCR) identify highly aggressive glioblastoma with poor outcome. Clinical Cancer Research, 24(6), 1448-1458.

Mitra, P., Guha, M., Ghosh, S., Mukherjee, S., Bankura, B., Pal, D. K., ... & Das, M. (2017). Association of calcitonin receptor gene (CALCR) polymorphism with kidney stone disease in the population of West Bengal, India. Gene, 622, 23-28.

Yuan, J., Gilbert, E. R., & Cline, M. A. (2017). The central anorexigenic mechanism of amylin in Japanese quail (Coturnix japonica) involves pro-opiomelanocortin, calcitonin receptor, and the arcuate nucleus of the hypothalamus. Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology, 210, 28-34.

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For research use only. Not intended for any clinical use.

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