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Mouse Anti-CALB2 Recombinant Antibody (CBFYC-0399) (CBMAB-C0440-FY)


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Published Data
CBMAB-C0440-FY-1
Mouse Anti-CALB2 Recombinant Antibody (CBFYC-0399) (CBMAB-C0440-FY) in IF
Co-expression of AUTS2 and GALNT17 in different cell types in CB. Expression of AUTS2 (red; top) and GALNT17 (red; bottom) in various cell types was determined by co-staining with antibodies to different markers, and by cell shape and location in CB. Both proteins are expressed in Bergmann glia (A and E; arrowheads) as marked by GFAP in green at P1, and in PCs (B and F; stars) as marked by Calbindin (CalB) in green at P60. Both proteins were also co-expressed in cerebellar GCs (B and F; asterisks), stellate cells (B and F; arrowheads), large neurons in the deep cerebellar nuclei (C and G; arrowheads), and choroid plexus (D and H). scale bar = 10 μm. ...View More
Weisner, P. A., Chen, C. Y., Sun, Y., Yoo, J., Kao, W. C., Zhang, H., ... & Stubbs, L. (2019). A mouse mutation that dysregulates neighboring Galnt17 and Auts2 genes is associated with phenotypes related to the human AUTS2 syndrome. G3: Genes, Genomes, Genetics, 9(11), 3891-3906.
CBMAB-C0440-FY-2
Mouse Anti-CALB2 Recombinant Antibody (CBFYC-0399) (CBMAB-C0440-FY) in IF
Immunofluorescence staining of Lsd1, various major cell specific markers, and TUNEL show decreased presence of specific retinal neurons and increased apoptosis in Chx10-Cre Lsd1fl/fl animals compared to controls. ...View More
Ferdous, S., Shelton, D. A., Getz, T. E., Chrenek, M. A., L'Hernault, N., Sellers, J. T., ... & Nickerson, J. M. (2023). Deletion of histone demethylase Lsd1 (Kdm1a) during retinal development leads to defects in retinal function and structure. Frontiers in Cellular Neuroscience, 17, 1104592.
CBMAB-C0440-FY-3
Mouse Anti-CALB2 Recombinant Antibody (CBFYC-0399) (CBMAB-C0440-FY) in IF
Left, Schematic of retinal circuit at P9 and P15. Middle, Immunofluorescence for TRPM3 and calretinin at two developmental ages. Calretinin is present in ACs, dACs, and some RGCs. White arrow, A TRPM3 immuno-positive putative AC. Right, Image intensity as a function of distance along the cross-section from the OPL for both TRPM3 (green) and calretinin (magenta). Bold lines are the average intensity from six ROIs and the light regions represent SD. Scale bar: 20 μm. ...View More
Webster, C. M., Tworig, J., Caval-Holme, F., Morgans, C. W., & Feller, M. B. (2020). The impact of steroid activation of TRPM3 on spontaneous activity in the developing retina. Eneuro, 7(2).

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
CBFYC-0399
Antibody Isotype
IgG1, κ
Application
ELISA, WB, IP, IF

Basic Information

Immunogen
Amino acids 2-27 at the N-terminus of Calretinin of human origin.
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG1, κ
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
ELISA1:100-1:1,000
IF(ICC)1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Calbindin 2
Introduction
This gene encodes an intracellular calcium-binding protein belonging to the troponin C superfamily. Members of this protein family have six EF-hand domains which bind calcium. This protein plays a role in diverse cellular functions, including message targeting and intracellular calcium buffering. It also functions as a modulator of neuronal excitability, and is a diagnostic marker for some human diseases, including Hirschsprung disease and some cancers. Alternative splicing results in multiple transcript variants.
Entrez Gene ID
794
UniProt ID
P22676
Alternative Names
Calbindin 2; 29 KDa Calbindin; Calretinin; CAB29; CR; Testicular Secretory Protein Li 8
Function
Calretinin is a calcium-binding protein which is abundant in auditory neurons.
Biological Process
Regulation of cytosolic calcium ion concentration Source: GO_Central
Regulation of long-term synaptic potentiation Source: GO_Central
Regulation of presynaptic cytosolic calcium ion concentration Source: GO_Central
Cellular Location
Cuticular plate; Cytosol; Nucleus; Synaptic membrane; Dendrite; Gap junction; Neuron projection; Parallel fiber to Purkinje cell synapse; Stereocilium; Synapse; Terminal bouton
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For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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