Search :
Sign in or Register  
Welcome Sign in or Don't have an account?Register

Rat Anti-C1QA Recombinant Antibody (7H8) (CBMAB-1234-CN)


See all C1QA antibodies
Online Inquiry
Published Data
CBMAB-1234-CN-1
CBMAB-1234-CN in Immunoblot
Immunoblotting band of CD68, C1qa, PSD95, SYN1, SYP, and β-actin (n = 3 per group). F Prolonged anesthesia increased the expression of C1qa. ...View More
Xu, F., Han, L., Wang, Y., Deng, D., Ding, Y., Zhao, S., ... & Chen, X. (2023). Prolonged anesthesia induces neuroinflammation and complement-mediated microglial synaptic elimination involved in neurocognitive dysfunction and anxiety-like behaviors. BMC medicine, 21(1), 7.
CBMAB-1234-CN-2
CBMAB-1234-CN in IF
Prolonged anesthesia induces microglia to secrete complement. Scale bar = 10 μm. R Prolonged anesthesia increased the number of microglia-colocalized C1qa (n = 4 per group). ...View More
Xu, F., Han, L., Wang, Y., Deng, D., Ding, Y., Zhao, S., ... & Chen, X. (2023). Prolonged anesthesia induces neuroinflammation and complement-mediated microglial synaptic elimination involved in neurocognitive dysfunction and anxiety-like behaviors. BMC medicine, 21(1), 7.
CBMAB-1234-CN-3
CBMAB-1234-CN in IF
Prolonged anesthesia increased complement-mediated synaptic elimination in the hippocampal CA1 region. Complement C1qa (green) colocalized with PSD95 (red) puncta was elevated after prolonged anesthesia. Scale bar = 10 μm. ...View More
Xu, F., Han, L., Wang, Y., Deng, D., Ding, Y., Zhao, S., ... & Chen, X. (2023). Prolonged anesthesia induces neuroinflammation and complement-mediated microglial synaptic elimination involved in neurocognitive dysfunction and anxiety-like behaviors. BMC medicine, 21(1), 7.
CBMAB-1234-CN-4
CBMAB-1234-CN in WB
Western blot analysis of PSD95 puncta in FACS-sorted IBA1+ microglia lysates from the co-cultures. NeuN, neuronal marker. ...View More
Hao, X., Li, Z., Li, W., Katz, J., Michalek, S. M., Barnum, S. R., ... & Zhang, P. (2022). Periodontal infection aggravates C1q-mediated microglial activation and synapse pruning in alzheimer's mice. Frontiers in immunology, 13, 816640.

Summary

Host Animal
Rat
Specificity
Human, Mouse, Rat
Clone
7H8
Antibody Isotype
IgG1
Application
IP, IC, IF, WB, FC

Basic Information

Immunogen
Serum-derived complement subcomponent C1q of mouse origin.
Host Species
Rat
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
0.09% sodium azide
Concentration
0.2 mg/ml
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
C1QA
Introduction
C1QA is composed of 18 polypeptide chains which include 6 A-chains, 6 B-chains, and 6 C-chains. Each chain contains an N-terminal collagen-like region and a C-terminal C1q globular domain. C1QA is the A-chain polypeptide of serum complement subcomponent C1q, which associates with C1r and C1s to yield the first component of the serum complement system. Deficiency of C1QA is associated with lupus erythematosus and glomerulonephritis.
Entrez Gene ID
Human712
Mouse12259
UniProt ID
HumanQ86Y26
MouseQ8BHP2
Alternative Names
C1q; AI255395
Function
C1q associates with the proenzymes C1r and C1s to yield C1, the first component of the serum complement system. The collagen-like regions of C1q interact with the Ca2+-dependent C1r2C1s2 proenzyme complex, and efficient activation of C1 takes place on interaction of the globular heads of C1q with the Fc regions of IgG or IgM antibody present in immune complexes.
Biological Process
Aging Source: ARUK-UCL
Astrocyte activation Source: ARUK-UCL
Cell-cell signaling Source: ProtInc
Complement activation Source: Reactome
Complement activation, classical pathway Source: Reactome
Complement-mediated synapse pruning Source: ARUK-UCL
Innate immune response Source: UniProtKB-KW
Microglial cell activation Source: ARUK-UCL
Neuron remodeling Source: ARUK-UCL
Positive regulation of neuron death Source: ARUK-UCL
Regulation of complement activation Source: Reactome
Response to iron ion Source: Ensembl
Synapse organization Source: ARUK-UCL
Synapse pruning Source: ARUK-UCL
Vertebrate eye-specific patterning Source: ARUK-UCL
Viral process Source: UniProtKB-KW
Cellular Location
Secreted
Involvement in disease
Complement component C1q deficiency (C1QD): A disorder caused by impaired activation of the complement classical pathway. It generally leads to severe immune complex disease with features of systemic lupus erythematosus and glomerulonephritis.
PTM
O-linked glycans are assumed to be the Glc-Gal disaccharides typically found as secondary modifications of hydroxylated lysines in collagen-like domains.
More Infomation
Ask a question We look forward to hearing from you.
0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Loading...
Have you used Rat Anti-C1QA Recombinant Antibody (7H8)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon $30 eGift Card
Submit a review
Loading...
For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Learn more

Documents

Request bulk or custom quoteOnline Inquiry
Second antibody and isotype control

Contact us

  • Tel: (USA)
  • (UK)
  • Fax:
  • Email:

Submit A Review

Online Inquiry