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Mouse Anti-Bub3 Recombinant Antibody (CBYY-2014) (CBMAB-2363-YY)


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Summary

Host Animal
Mouse
Specificity
Human
Clone
CBYY-2014
Antibody Isotype
IgG2b, κ
Application
E, WB

Basic Information

Immunogen
Recombinant human BUB3 (1-328aa) purified from E. coli.
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG2b, κ
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.4, 10% Glycerol
Preservative
0.02% sodium azide
Concentration
1 mg/ml
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
BUB3, Mitotic Checkpoint Protein
Introduction
This gene encodes a protein involved in spindle checkpoint function. The encoded protein contains four WD repeat domains and has sequence similarity with the yeast BUB3 protein. Alternate transcriptional splice variants, encoding different isoforMouse, have been characterized.
Entrez Gene ID
Human9184
Mouse12237
Rat361662
UniProt ID
HumanO43684
MouseQ9WVA3
RatD4A567
Alternative Names
BUB3, Mitotic Checkpoint Protein; BUB3 (Budding Uninhibited By Benzimidazoles 3, Yeast) Homolog; Budding Uninhibited By Benzimidazoles 3 Homolog (Yeast); BUB3 Budding Uninhibited By Benzimidazoles 3 Homolog; Budding Uninhibited By Benzimidazoles 3 Homolog; Mitotic Checkpoint Protein BUB3;
Function
Has a dual function in spindle-assembly checkpoint signaling and in promoting the establishment of correct kinetochore-microtubule (K-MT) attachments. Promotes the formation of stable end-on bipolar attachments. Necessary for kinetochore localization of BUB1. Regulates chromosome segregation during oocyte meiosis. The BUB1/BUB3 complex plays a role in the inhibition of anaphase-promoting complex or cyclosome (APC/C) when spindle-assembly checkpoint is activated and inhibits the ubiquitin ligase activity of APC/C by phosphorylating its activator CDC20. This complex can also phosphorylate MAD1L1.
Biological Process
Anaphase-promoting complex-dependent catabolic process Source: Reactome
Attachment of spindle microtubules to kinetochore Source: UniProtKB
Cell division Source: UniProtKB-KW
Meiotic cell cycle Source: UniProtKB-KW
Mitotic sister chromatid segregation Source: Ensembl
Mitotic spindle assembly checkpoint Source: GO_Central
Mitotic spindle organization Source: Reactome
Protein localization to kinetochore Source: UniProtKB
Regulation of exit from mitosis Source: Reactome
Regulation of mitotic cell cycle phase transition Source: Reactome
Ubiquitin-dependent protein catabolic process Source: Reactome
Cellular Location
Nucleus; Kinetochore. Starts to localize at kinetochores in prometaphase I (Pro-MI) stage and maintains the localization until the metaphase I-anaphase I (MI-AI) transition.
PTM
Poly-ADP-ribosylated by PARP1.
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For research use only. Not intended for any clinical use.

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