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Mouse Anti-BUB1B Recombinant Antibody (CBYY-0953) (CBMAB-0956-YY)


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Summary

Host Animal
Mouse
Specificity
Human, Rat
Clone
CBYY-0953
Antibody Isotype
IgG2b
Application
WB, IH, FC

Basic Information

Immunogen
E.coli-derived human BubR1/BUB1B recombinant protein (Position: K26-E448).
Host Species
Mouse
Specificity
Human, Rat
Antibody Isotype
IgG2b
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB0.1-0.5 μg/ml
IHC0.5-1 μg/ml
FC1-3 μg/10^6 cells

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Lyophilized
Buffer
PBS, Trehalose
Preservative
None
Concentration
0.4 mg/mL
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
BUB1 Mitotic Checkpoint Serine/Threonine Kinase B
Introduction
This gene encodes a kinase involved in spindle checkpoint function. The protein has been localized to the kinetochore and plays a role in the inhibition of the anaphase-promoting complex/cyclosome (APC/C), delaying the onset of anaphase and ensuring proper chromosome segregation. Impaired spindle checkpoint function has been found in many forMouse of cancer.
Entrez Gene ID
Human701
Dog608350
Rat171576
Monkey100423079
UniProt ID
HumanO60566
DogF1PLV9
RatF1LMI1
MonkeyQ4R5Y6
Alternative Names
BUB1 Mitotic Checkpoint Serine/Threonine Kinase B; MAD3/BUB1-Related Protein Kinase; Mitotic Checkpoint Kinase MAD3L; HBUBR1; BUBR1; MAD3L; SSK1; Budding Uninhibited By Benzimidazoles 1 (Yeast Homolog), Beta; Budding Uninhibited By Benzimidazoles 1 Homolog Beta (Yeast);
Function
Essential component of the mitotic checkpoint. Required for normal mitosis progression. The mitotic checkpoint delays anaphase until all chromosomes are properly attached to the mitotic spindle. One of its checkpoint functions is to inhibit the activity of the anaphase-promoting complex/cyclosome (APC/C) by blocking the binding of CDC20 to APC/C, independently of its kinase activity. The other is to monitor kinetochore activities that depend on the kinetochore motor CENPE. Required for kinetochore localization of CENPE. Negatively regulates PLK1 activity in interphase cells and suppresses centrosome amplification. Also implicated in triggering apoptosis in polyploid cells that exit aberrantly from mitotic arrest. May play a role for tumor suppression.
Biological Process
Anaphase-promoting complex-dependent catabolic process Source: Reactome
Apoptotic process Source: UniProtKB-KW
Cell division Source: UniProtKB-KW
Meiotic sister chromatid cohesion, centromeric Source: GO_Central
Metaphase/anaphase transition of mitotic cell cycle Source: Ensembl
Mitotic cell cycle Source: ProtInc
Mitotic cell cycle checkpoint Source: ProtInc
Mitotic spindle assembly checkpoint Source: GO_Central
Mitotic spindle organization Source: Reactome
Protein localization to chromosome, centromeric region Source: Ensembl
Regulation of exit from mitosis Source: Reactome
Regulation of mitotic cell cycle phase transition Source: Reactome
Ubiquitin-dependent protein catabolic process Source: Reactome
Cellular Location
Cytoplasm; Nucleus; Centrosome; Kinetochore. Cytoplasmic in interphase cells. Associates with the kinetochores in early prophase. Kinetochore localization requires BUB1, PLK1 and KNL1.
Involvement in disease
Defects in BUB1B are associated with tumor formation.
Premature chromatid separation trait (PCS): Consists of separate and splayed chromatids with discernible centromeres and involves all or most chromosomes of a metaphase. It is found in up to 2% of metaphases in cultured lymphocytes from approximately 40% of normal individuals. When PCS is present in 5% or more of cells, it is known as the heterozygous PCS trait and has no obvious phenotypic effect, although some have reported decreased fertility. Inheritance is autosomal dominant.
Mosaic variegated aneuploidy syndrome 1 (MVA1): A severe developmental disorder characterized by mosaic aneuploidies, predominantly trisomies and monosomies, involving multiple different chromosomes and tissues. Affected individuals typically present with severe intrauterine growth retardation and microcephaly. Eye anomalies, mild dysmorphism, variable developmental delay, and a broad spectrum of additional congenital abnormalities and medical conditions may also occur. The risk of malignancy is high, with rhabdomyosarcoma, Wilms tumor and leukemia reported in several cases.
PTM
Proteolytically cleaved by caspase-3 in a cell cycle specific manner. The cleavage might be involved in the durability of the cell cycle delay. Caspase-3 cleavage is associated with abrogation of the mitotic checkpoint. The major site of cleavage is at Asp-610.
Acetylation at Lys-250 regulates its degradation and timing in anaphase entry.
Ubiquitinated. Degraded by the proteasome.
Sumoylated with SUMO2 and SUMO3. The sumoylation mediates the association with CENPE at the kinetochore.
Autophosphorylated in vitro. Intramolecular autophosphorylation is stimulated by CENPE. Phosphorylated during mitosis and hyperphosphorylated in mitotically arrested cells. Phosphorylation at Ser-670 and Ser-1043 oCcurs at kinetochores upon mitotic entry with dephosphorylation at the onset of anaphase.
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For research use only. Not intended for any clinical use.

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