Rabbit Anti-BRDT Recombinant Antibody (CBYY-0809) (CBMAB-0812-YY)

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Datasheet

Summary

Host Animal

Rabbit

Specificity

Human

Clone

CBYY-0809

Antibody Isotype

IgG

Application

WB, IP

Basic Information

Immunogen

Recombinant protein specific to the carboxy terminus of human BRDT protein.

Host Species

Rabbit

Specificity

Human

Antibody Isotype

IgG

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:1,000
IP	1:100

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

HEPES, pH 7.5, 100 µg/ml BSA, 50% glycerol

Preservative

0.02% sodium azide

Concentration

Batch dependent

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name

BRDT

Introduction

BRDT is similar to the RING3 protein family. It possesses 2 bromodomain motifs and a PEST sequence (a cluster of proline, glutamic acid, serine, and threonine residues), characteristic of proteins that undergo rapid intracellular degradation. The bromodomain is found in proteins that regulate transcription. Several transcript variants encoding multiple isoforMouse have been found for this gene.

Entrez Gene ID

676

UniProt ID

Q58F21

Alternative Names

Bromodomain Testis Associated; Cancer/Testis Antigen 9; Bromodomain, Testis-Specific; RING3-Like Protein; CT9; Bromodomain Testis-Specific Protein; SPGF21; BRD6;

Function

Testis-specific chromatin protein that specifically binds histone H4 acetylated at 'Lys-5' and 'Lys-8' (H4K5ac and H4K8ac, respectively) and plays a key role in spermatogenesis (PubMed:22464331, PubMed:22901802).
Required in late pachytene spermatocytes: plays a role in meiotic and post-meiotic cells by binding to acetylated histones at the promoter of specific meiotic and post-meiotic genes, facilitating their activation at the appropriate time (PubMed:22901802).
In the post-meiotic phase of spermatogenesis, binds to hyperacetylated histones and participates in their general removal from DNA (PubMed:22901802).
Also recognizes and binds a subset of butyrylated histones: able to bind histone H4 butyrylated at 'Lys-8' (H4K8ac), while it is not able to bind H4 butyrylated at 'Lys-5' (H4K5ac) (By similarity).
Also acts as a component of the splicing machinery in pachytene spermatocytes and round spermatids and participates in 3'-UTR truncation of specific mRNAs in post-meiotic spermatids (By similarity).
Required for chromocenter organization, a structure comprised of peri-centromeric heterochromatin.

Biological Process

Cell differentiation Source: UniProtKB-KW
Chromatin remodeling Source: UniProtKB
Histone displacement Source: UniProtKB
Male meiosis I Source: UniProtKB
Male meiotic nuclear division Source: UniProtKB
mRNA processing Source: UniProtKB-KW
Positive regulation of transcription involved in meiotic cell cycle Source: UniProtKB
Regulation of RNA splicing Source: UniProtKB
Regulation of transcription, DNA-templated Source: UniProtKB
RNA splicing Source: UniProtKB-KW
Spermatogenesis Source: UniProtKB

Cellular Location

Nucleus.Detected on chromatin.

Involvement in disease

Spermatogenic failure 21 (SPGF21): An infertility disorder caused by spermatogenesis defects and characterized by acephalic spermatozoa in the semen of affected individuals. SPGF21 inheritance is autosomal recessive.

More Infomation

References

Wang, X., Kutschat, A. P., Yamada, M., Prokakis, E., Böttcher, P., Tanaka, K., ... & Johnsen, S. A. (2021). Bromodomain protein BRDT directs ΔNp63 function and super-enhancer activity in a subset of esophageal squamous cell carcinomas. Cell Death & Differentiation, 1-14.

Chen, L., Cai, S., Wang, J. M., Huai, Y. Y., Lu, P. H., & Chu, Q. (2020). BRDT promotes ovarian cancer cell growth. Cell death & disease, 11(11), 1-14.

Wang, X., Hamdan, F. H., Sen, M., Kutschat, A. P., & Johnsen, S. A. (2018). Bromodomain testis-specific protein BRDT is expressed in a subset of esophageal squamous cell carcinomas and controls expression of differentiation-associated genes.

Bourova-Flin, E., Chuffart, F., Rousseaux, S., & Khochbin, S. (2017). The role of bromodomain testis-specific factor, BRDT, in cancer: a biomarker and a possible therapeutic target. Cell Journal (Yakhteh), 19(Suppl 1), 1.

Ekaterina, B. F., Florent, C., Sophie, R., & Saadi, K. (2017). < The> role of bromodomain testis-specific factor, brdt, in cancer: a biomarker and a possible therapeutic target.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Associated Products

Related Products

Mouse Anti-BRDT Recombinant Antibody (0410)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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