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Mouse Anti-BRDT Recombinant Antibody (0410) (CBMAB-0410-CN)


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Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
0410
Antibody Isotype
IgG1, κ
Application
WB, IP, IF, E

Basic Information

Immunogen
Amino acids 820-851 mapping near the C-terminus of BRDT of human origin.
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
ELISA1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
0.09% sodium azide
Concentration
0.2 mg/ml
Purity
>95%, as determined by SDS-PAGE analysis
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
BRDT
Introduction
BRDT is similar to the RING3 protein family. It possesses 2 bromodomain motifs and a PEST sequence (a cluster of proline, glutamic acid, serine, and threonine residues), characteristic of proteins that undergo rapid intracellular degradation. Testis-specific chromatin protein that specifically binds histone H4 acetylated at 'Lys-5' and 'Lys-8' (H4K5ac and H4K8ac, respectively) and plays a key role in spermatogenesis. Required in late pachytene spermatocytes: plays a role in meiotic and post-meiotic cells by binding to acetylated histones at the promoter of specific meiotic and post-meiotic genes, facilitating their activation at the appropriate time
Entrez Gene ID
676
UniProt ID
Q58F21
Alternative Names
CT9; BRD6; SPGF21
Function
Testis-specific chromatin protein that specifically binds histone H4 acetylated at 'Lys-5' and 'Lys-8' (H4K5ac and H4K8ac, respectively) and plays a key role in spermatogenesis (PubMed:22464331, PubMed:22901802).
Required in late pachytene spermatocytes: plays a role in meiotic and post-meiotic cells by binding to acetylated histones at the promoter of specific meiotic and post-meiotic genes, facilitating their activation at the appropriate time (PubMed:22901802).
In the post-meiotic phase of spermatogenesis, binds to hyperacetylated histones and participates in their general removal from DNA (PubMed:22901802).
Also recognizes and binds a subset of butyrylated histones: able to bind histone H4 butyrylated at 'Lys-8' (H4K8ac), while it is not able to bind H4 butyrylated at 'Lys-5' (H4K5ac) (By similarity).
Also acts as a component of the splicing machinery in pachytene spermatocytes and round spermatids and participates in 3'-UTR truncation of specific mRNAs in post-meiotic spermatids (By similarity).
Required for chromocenter organization, a structure comprised of peri-centromeric heterochromatin.
Biological Process
Cell differentiation Source: UniProtKB-KW
Chromatin remodeling Source: UniProtKB
Histone displacement Source: UniProtKB
Male meiosis I Source: UniProtKB
Male meiotic nuclear division Source: UniProtKB
mRNA processing Source: UniProtKB-KW
Positive regulation of transcription involved in meiotic cell cycle Source: UniProtKB
Regulation of RNA splicing Source: UniProtKB
Regulation of transcription, DNA-templated Source: UniProtKB
RNA splicing Source: UniProtKB-KW
Spermatogenesis Source: UniProtKB
Cellular Location
Nucleus.Detected on chromatin.
Involvement in disease
Spermatogenic failure 21 (SPGF21): An infertility disorder caused by spermatogenesis defects and characterized by acephalic spermatozoa in the semen of affected individuals. SPGF21 inheritance is autosomal recessive.
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For research use only. Not intended for any clinical use.

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