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Mouse Anti-BMX Recombinant Antibody (CBFYE-0312) (CBMAB-E0595-FY)


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Summary

Host Animal
Mouse
Specificity
Human, Mouse, Dog, Rat, Pig, Cattle
Clone
CBFYE-0312
Antibody Isotype
IgG3, κ
Application
WB, IP, IF, E

Basic Information

Immunogen
Amino acids 643-671 at the C-terminus of Bmx of human origin.
Host Species
Mouse
Specificity
Human, Mouse, Dog, Rat, Pig, Cattle
Antibody Isotype
IgG3, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
ELISA1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
0.09% sodium azide
Concentration
0.2 mg/ml
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
BMX Non-Receptor Tyrosine Kinase
Introduction
This gene encodes a non-receptor tyrosine kinase belonging to the Tec kinase family. The protein contains a PH-like domain, which mediates membrane targeting by binding to phosphatidylinositol 3,4,5-triphosphate (PIP3), and a SH2 domain that binds to tyrosine-phosphorylated proteins and functions in signal transduction. The protein is implicated in several signal transduction pathways including the Stat pathway, and regulates differentiation and tumorigenicity of several types of cancer cells. Alternatively spliced transcript variants have been found for this gene.
Entrez Gene ID
660
UniProt ID
P51813
Alternative Names
BMX Non-Receptor Tyrosine Kinase; Bone Marrow Tyrosine Kinase Gene In Chromosome X Protein; Epithelial And Endothelial Tyrosine Kinase; BTK-Like On X Chromosome; EC 2.7.10.2; ETK; Cytoplasmic Tyrosine-Protein Kinase BMX
Function
Non-receptor tyrosine kinase that plays central but diverse modulatory roles in various signaling processes involved in the regulation of actin reorganization, cell migration, cell proliferation and survival, cell adhesion, and apoptosis. Participates in signal transduction stimulated by growth factor receptors, cytokine receptors, G-protein coupled receptors, antigen receptors and integrins. Induces tyrosine phosphorylation of BCAR1 in response to integrin regulation. Activation of BMX by integrins is mediated by PTK2/FAK1, a key mediator of integrin signaling events leading to the regulation of actin cytoskeleton and cell motility. Plays a critical role in TNF-induced angiogenesis, and implicated in the signaling of TEK and FLT1 receptors, 2 important receptor families essential for angiogenesis. Required for the phosphorylation and activation of STAT3, a transcription factor involved in cell differentiation. Also involved in interleukin-6 (IL6) induced differentiation. Plays also a role in programming adaptive cytoprotection against extracellular stress in different cell systems, salivary epithelial cells, brain endothelial cells, and dermal fibroblasts. May be involved in regulation of endocytosis through its interaction with an endosomal protein RUFY1. May also play a role in the growth and differentiation of hematopoietic cells; as well as in signal transduction in endocardial and arterial endothelial cells.
Biological Process
Adaptive immune response Source: GO_Central
Apoptotic process Source: UniProtKB-KW
B cell receptor signaling pathway Source: GO_Central
Cell adhesion Source: UniProtKB-KW
Intracellular signal transduction Source: InterPro
Mesoderm development Source: ProtInc
Peptidyl-tyrosine autophosphorylation Source: GO_Central
Phosphatidylinositol biosynthetic process Source: Reactome
Protein autophosphorylation Source: UniProtKB
Protein phosphorylation Source: ProtInc
Signal transduction Source: UniProtKB
Cellular Location
Cytoplasm. Localizes to the edges of spreading cells when complexed with BCAR1.
PTM
Phosphorylated in response to protein I/II and to LPS. Phosphorylation at Tyr-566 by SRC and by autocatalysis leads to activation and is required for STAT3 phosphorylation by BMX.
More Infomation

Yang, F., Duan, M., Zheng, F., Yu, L., Wang, Y., Wang, G., ... & Zhu, S. (2021). Fas signaling in adipocytes promotes low-grade inflammation and lung metastasis of colorectal cancer through interaction with Bmx. Cancer Letters.

Sousa, B. B., Sousa, M. F., Marques, M. C., Seixas, J. D., Brito, J. A., Matias, P. M., ... & Roldão, A. (2021). A scalable insect cell-based production process of the human recombinant BMX for in-vitro covalent ligand high-throughput screening. Bioprocess and Biosystems Engineering, 44(1), 209-215.

Mamoor, S. (2020). Tyrosine kinase BMX is differentially expressed in the brain metastases of patients with metastatic breast cancer.

Seixas, J. D., Sousa, B. B., Marques, M. C., Guerreiro, A., Traquete, R., Rodrigues, T., ... & Bernardes, G. J. (2020). Structural and biophysical insights into the mode of covalent binding of rationally designed potent BMX inhibitors. RSC Chemical Biology, 1(4), 251-262.

Seixas, J. D., Sousa, B. B., Marques, M. C., Guerreiro, A., Traquete, R., Rodrigues, T., ... & Bernardes, G. (2020). Rationally designed potent BMX inhibitors reveals mode of covalent binding at the atomic level.

Li, K., Pan, W. T., Ma, Y. B., Xu, X. L., Gao, Y., He, Y. Q., ... & Zhang, J. W. (2020). BMX activates Wnt/β-catenin signaling pathway to promote cell proliferation and migration in breast cancer. Breast cancer, 27(3), 363-371.

Wang, Y., Xia, J., Fang, Z., Li, F., Li, D., Wang, Z., ... & Liu, H. (2017). A novel BMX variant promotes tumor cell growth and migration in lung adenocarcinoma. Oncotarget, 8(20), 33405.

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For research use only. Not intended for any clinical use.

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