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Mouse Anti-BLK Recombinant Antibody (3E5-3A8) (CBMAB-A0796-LY)


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Summary

Host Animal
Mouse
Specificity
Human
Clone
3E5-3A8
Antibody Isotype
IgG1, κ
Application
WB, E

Basic Information

Immunogen
BLK (AAH07371, 1 a.a. ~ 505 a.a) full-length recombinant protein with GST tag.
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.4
Preservative
None
Concentration
Batch dependent
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
BLK Proto-Oncogene, Src Family Tyrosine Kinase
Entrez Gene ID
640
UniProt ID
P51451
Alternative Names
MGC10442
Function
Non-receptor tyrosine kinase involved in B-lymphocyte development, differentiation and signaling (By similarity).
B-cell receptor (BCR) signaling requires a tight regulation of several protein tyrosine kinases and phosphatases, and associated coreceptors (By similarity).
Binding of antigen to the B-cell antigen receptor (BCR) triggers signaling that ultimately leads to B-cell activation (By similarity).
Signaling through BLK plays an important role in transmitting signals through surface immunoglobulins and supports the pro-B to pre-B transition, as well as the signaling for growth arrest and apoptosis downstream of B-cell receptor (By similarity).
Specifically binds and phosphorylates CD79A at 'Tyr-188'and 'Tyr-199', as well as CD79B at 'Tyr-196' and 'Tyr-207' (By similarity).
Phosphorylates also the immunoglobulin G receptors FCGR2A, FCGR2B and FCGR2C (PubMed:8756631).
With FYN and LYN, plays an essential role in pre-B-cell receptor (pre-BCR)-mediated NF-kappa-B activation (By similarity).
Contributes also to BTK activation by indirectly stimulating BTK intramolecular autophosphorylation (By similarity).
In pancreatic islets, acts as a modulator of beta-cells function through the up-regulation of PDX1 and NKX6-1 and consequent stimulation of insulin secretion in response to glucose (PubMed:19667185).
Phosphorylates CGAS, promoting retention of CGAS in the cytosol (PubMed:30356214).
Biological Process
B cell receptor signaling pathway Source: UniProtKB
Cell differentiation Source: GO_Central
Cellular response to DNA damage stimulus Source: GO_Central
Dendritic cell differentiation Source: GO_Central
Fc receptor mediated stimulatory signaling pathway Source: GO_Central
Innate immune response Source: GO_Central
Intracellular signal transduction Source: ProtInc
Negative regulation of B cell proliferation Source: GO_Central
Negative regulation of mast cell proliferation Source: GO_Central
Neuron projection development Source: GO_Central
Peptidyl-tyrosine phosphorylation Source: UniProtKB
Platelet degranulation Source: GO_Central
Positive regulation of dendritic cell apoptotic process Source: GO_Central
Positive regulation of insulin secretion Source: UniProtKB
Regulation of B cell apoptotic process Source: GO_Central
Regulation of B cell receptor signaling pathway Source: GO_Central
Regulation of mast cell degranulation Source: GO_Central
Regulation of platelet aggregation Source: GO_Central
Tolerance induction to self antigen Source: GO_Central
Transmembrane receptor protein tyrosine kinase signaling pathway Source: GO_Central
Cellular Location
Cell membrane. Present and active in lipid rafts. Membrane location is required for the phosphorylation of CD79A and CD79B (By similarity).
Involvement in disease
Maturity-onset diabetes of the young 11 (MODY11): A form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease.
PTM
Phosphorylated on tyrosine residues after antibody-mediated surface engagement of the B-cell antigen receptor (BCR).
Ubiquitination of activated BLK by the UBE3A ubiquitin protein ligase leads to its degradation by the ubiquitin-proteasome pathway.
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For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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