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Mouse Anti-BIRC5 Recombinant Antibody (6E4) (CBMAB-CP2646-LY)


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Published Data
CBMAB-CP2646-LY-1
CBMAB-CP2646-LY in WB
PDAC cells were grown for 27 h, then lysed and subjected to western blot analyses for FLIP, Bcl-xL, Mcl-1, Survivin, XIAP, cIAP1, and cIAP2. Blots are representative, where Colo357 cells and Panc1 cells were compared side by side. ...View More
Hagelund, S., & Trauzold, A. (2022). Impact of extracellular pH on apoptotic and non-apoptotic TRAIL-Induced signaling in pancreatic ductal adenocarcinoma cells. Frontiers in Cell and Developmental Biology, 10, 768579.
CBMAB-CP2646-LY-2
CBMAB-CP2646-LY in WB
Effect of Helle on the expression level of cell cycle related-proteins in MCF-7 cells. After treatment with various concentrations of Helle (3, 10, 30 and 100 nM) for 48 h, the expression levels of cell cycle-related proteins were analyzed by western blotting. ...View More
Zhang, Y., Yuan, B., Bian, B., Zhao, H., Kiyomi, A., Hayashi, H., ... & Takagi, N. (2021). Cytotoxic effects of hellebrigenin and arenobufagin against human breast cancer cells. Frontiers in Oncology, 11, 711220.
CBMAB-CP2646-LY-3
CBMAB-CP2646-LY in WB
The expression levels were expressed as the ratios between each targeted protein and β-actin protein expression levels, and were compared with those of control group. ∫p < 0.05; #p < 0.01; †p < 0.001; ♪p < 0.0001 vs. each alone. As, AsIII; Gama, gamabufotalin. ...View More
Yuan, B., Xu, K., Shimada, R., Li, J., Hayashi, H., Okazaki, M., & Takagi, N. (2021). Cytotoxic Effects of Arsenite in Combination With Gamabufotalin Against Human Glioblastoma Cell Lines. Frontiers in oncology, 11, 628914.
CBMAB-CP2646-LY-4
CBMAB-CP2646-LY in WB
Confirmation of survivin inhibition by Western blotting. (a) The expression of survivin in DLD-1 and A549 cells treated with 5 μg/mL marine samples or controls for 24 h was ascertained using Western blotting. The ability of the compounds to reduce survivin expression in DLD-1 and A549 cells was confirmed. Western blot for one representative experiment is shown. (b) Densitometry analysis showed that this decrease was significant (p < 0.05) in samples marked with an asterisk. Graph shows the average densitometry ± standard deviation for three experiments. ...View More
Guzmán, E. A., Pitts, T. P., Tandberg, K. R., Winder, P. L., & Wright, A. E. (2021). Discovery of Survivin inhibitors part 1: screening the Harbor Branch pure compound Library. Marine Drugs, 19(2), 73.
CBMAB-CP2646-LY-5
CBMAB-CP2646-LY in Immunoblot
Phosphorylation/expression of Akt, p38, Bax, Mcl-1, Bcl-2, and survivin in SCC4 cells. Cells were treated with IQ for 48 h, and cell lysates were immunoblotted as described in Materials and Methods. ...View More
Lin, C. W., Bai, L. Y., Su, J. H., Chiu, C. F., Lin, W. Y., Huang, W. T., ... & Weng, J. R. (2020). Ilimaquinone induces apoptosis and autophagy in human oral squamous cell carcinoma cells. Biomedicines, 8(9), 296.
CBMAB-CP2646-LY-6
CBMAB-CP2646-LY in WB
Western blot analysis of proliferative and apoptotic markers following LEE011 and ruxolitinib combined treatment. Cells were singly and combine treated to LEE011 and ruxolitinib at concentrations as indicated. Protein lysates from 10 and 24 h were ran on the same SDS-PAGE gel, while that of 48 and 72 h were ran on a separate gel. In general, a decrease in proliferative markers and increase in apoptotic markers in combine treatment as compared to single treatment were noted across different time points. ...View More
Hee, Y. T., Yan, J., Nizetic, D., & Chng, W. J. (2018). LEE011 and ruxolitinib: a synergistic drug combination for natural killer/T-cell lymphoma (NKTCL). Oncotarget, 9(61), 31832.
CBMAB-CP2646-LY-7
CBMAB-CP2646-LY in Immunoblot
The expression levels of apoptosis-associated proteins were detected by immunoblotting. Statistical significance: *p < 0.05, **p < 0.01, ***p < 0.001. ...View More
Li, X., Lu, J., Kan, Q., Li, X., Fan, Q., Li, Y., ... & Suo, Z. (2017). Metabolic reprogramming is associated with flavopiridol resistance in prostate cancer DU145 cells. Scientific reports, 7(1), 5081.

Summary

Host Animal
Mouse
Specificity
Human, Monkey
Clone
6E4
Antibody Isotype
IgG1
Application
WB

Basic Information

Immunogen
Synthetic peptide corresponding to the amino-terminal sequence of human survivin.
Host Species
Mouse
Specificity
Human, Monkey
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
HEPES, pH 7.5, 100 µg/ml BSA, 50% glycerol
Preservative
0.02% sodium azide
Concentration
Batch dependent
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Baculoviral IAP Repeat Containing 5
Introduction
This gene is a member of the inhibitor of apoptosis (IAP) gene family, which encode negative regulatory proteins that prevent apoptotic cell death. IAP family members usually contain multiple baculovirus IAP repeat (BIR) domains, but this gene encodes proteins with only a single BIR domain. The encoded proteins also lack a C-terminus RING finger domain. Gene expression is high during fetal development and in most tumors, yet low in adult tissues. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jun 2011]
Entrez Gene ID
Human332
Monkey709565
UniProt ID
HumanO15392
MonkeyA3E101
Alternative Names
Baculoviral IAP Repeat Containing 5; Apoptosis Inhibitor 4; Apoptosis Inhibitor Survivin; Survivin Variant 3 Alpha; API4; Baculoviral IAP Repeat-Containing Protein 5; Baculoviral IAP Repeat-Containing 5; EPR-1; IAP4;
Function
Multitasking protein that has dual roles in promoting cell proliferation and preventing apoptosis (PubMed:9859993, PubMed:21364656, PubMed:20627126, PubMed:25778398, PubMed:28218735).
Component of a chromosome passage protein complex (CPC) which is essential for chromosome alignment and segregation during mitosis and cytokinesis (PubMed:16322459).
Acts as an important regulator of the localization of this complex; directs CPC movement to different locations from the inner centromere during prometaphase to midbody during cytokinesis and participates in the organization of the center spindle by associating with polymerized microtubules (PubMed:20826784).
Involved in the recruitment of CPC to centromeres during early mitosis via association with histone H3 phosphorylated at 'Thr-3' (H3pT3) during mitosis (PubMed:20929775).
The complex with RAN plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules (PubMed:18591255).
May counteract a default induction of apoptosis in G2/M phase (PubMed:9859993).
The acetylated form represses STAT3 transactivation of target gene promoters (PubMed:20826784).
May play a role in neoplasia (PubMed:10626797).
Inhibitor of CASP3 and CASP7 (PubMed:21536684).
Essential for the maintenance of mitochondrial integrity and function (PubMed:25778398).
Isoform 2 and isoform 3 do not appear to play vital roles in mitosis (PubMed:12773388, PubMed:16291752).
Isoform 3 shows a marked reduction in its anti-apoptotic effects when compared with the displayed wild-type isoform (PubMed:10626797).
Biological Process
Cell division Source: UniProtKB
Chromosome segregation Source: UniProtKB-KW
Cytokine-mediated signaling pathway Source: Reactome
Establishment of chromosome localization Source: UniProtKB
G2/M transition of mitotic cell cycle Source: UniProtKB
Mitotic cell cycle Source: UniProtKB
Mitotic cytokinesis Source: UniProtKB
Mitotic spindle assembly checkpoint Source: UniProtKB
Mitotic spindle organization Source: Reactome
Negative regulation of apoptotic process Source: UniProtKB
Negative regulation of cysteine-type endopeptidase activity involved in apoptotic process Source: UniProtKB
Negative regulation of transcription, DNA-templated Source: UniProtKB
Positive regulation of cell population proliferation Source: UniProtKB
Positive regulation of exit from mitosis Source: UniProtKB
Positive regulation of mitotic cell cycle Source: UniProtKB
Protein-containing complex localization Source: UniProtKB
Protein phosphorylation Source: UniProtKB
Regulation of apoptotic process Source: Reactome
Regulation of cell cycle Source: GO_Central
Sensory perception of sound Source: UniProtKB
Cellular Location
Spindle; Cytoplasm; Nucleus; Chromosome; Centromere; Kinetochore; Midbody. Localizes at the centromeres from prophase to metaphase, at the spindle midzone during anaphase and a the midbody during telophase and cytokinesis. Accumulates in the nucleus upon treatment with leptomycin B (LMB), a XPO1/CRM1 nuclear export inhibitor (By similarity). Localizes on chromosome arms and inner centromeres from prophase through metaphase. Localizes to kinetochores in metaphase, distributes to the midzone microtubules in anaphase and at telophase, localizes exclusively to the midbody (PubMed:11084331). Colocalizes with AURKB at mitotic chromosomes (PubMed:14610074). Acetylation at Lys-129 directs its localization to the nucleus by enhancing homodimerization and thereby inhibiting XPO1/CRM1-mediated nuclear export (PubMed:20826784).
PTM
Ubiquitinated by the Cul9-RING ubiquitin-protein ligase complex, leading to its degradation. Ubiquitination is required for centrosomal targeting.
In vitro phosphorylation at Thr-117 by AURKB prevents interaction with INCENP and localization to mitotic chromosomes (PubMed:14610074). Phosphorylation at Thr-48 by CK2 is critical for its mitotic and anti-apoptotic activities (PubMed:21252625). Phosphorylation at Thr-34 by CDK15 is critical for its anti-apoptotic activity (PubMed:24866247). Phosphorylation at Ser-20 by AURKC is critical for regulation of proper chromosome alignment and segregation, and possibly cytokinesis.
Acetylation at Lys-129 by CBP results in its homodimerization, while deacetylation promotes the formation of monomers which heterodimerize with XPO1/CRM1 which facilitates its nuclear export. The acetylated form represses STAT3 transactivation. The dynamic equilibrium between its acetylation and deacetylation at Lys-129 determines its interaction with XPO1/CRM1, its subsequent subcellular localization, and its ability to inhibit STAT3 transactivation.
More Infomation

He, D., Huang, K., & Liang, Z. (2021). Prognostic value of baculoviral IAP repeat containing 5 expression as a new biomarker in lung adenocarcinoma: a meta-analysis. Expert Review of Molecular Diagnostics, 1-9.

Mamoor, S. (2021). Over-expression of baculoviral IAP repeat containing 5 in human endometrial cancer.

Pahlavan, Y., & Khabbazi, A. (2021). Baculoviral inhibitor of apoptosis family of proteins repeat-containing 5 gene methylation status in peripheral blood mononuclear cells and plasma survivin levels in patients with Behçet’s disease. Archives of Rheumatology, 36(2).

Basha, R., Tran, N., & Ibarra-Aleman, V. (2021). Association of baculoviral inhibitor of apoptosis repeat-containing 5 in the survival of clear cell renal cell carcinoma patients: In silico analysis.

Li, Y., Zhao, Z. G., Luo, Y., Cui, H., Wang, H. Y., Jia, Y. F., & Gao, Y. T. (2020). Dual targeting of Polo-like kinase 1 and baculoviral inhibitor of apoptosis repeat-containing 5 in TP53-mutated hepatocellular carcinoma. World Journal of Gastroenterology, 26(32), 4786.

Zou, J., Liao, X., Zhang, J., & Wang, L. (2019). Dysregulation of miR-195-5p/-218-5p/BIRC5 axis predicts a poor prognosis in patients with gastric cancer. Journal of biological regulators and homeostatic agents, 33(5), 1377-1385.

Sušac, I., Ozretić, P., Gregorić, M., Levačić Cvok, M., Sabol, M., Levanat, S., ... & Musani, V. (2019). Polymorphisms in Survivin (BIRC5 Gene) are associated with age of onset in breast cancer patients. Journal of oncology, 2019.

Zhao, H., Zhang, X., Han, Z., Wang, Z., & Wang, Y. (2018). Plasma anti‐BIRC 5 IgG may be a useful marker for evaluating the prognosis of nonsmall cell lung cancer. FEBS Open Bio, 8(5), 829-835.

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For research use only. Not intended for any clinical use.

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