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Mouse Anti-BIRC5 Monoclonal Antibody (60.11) (CBMAB-1576-YC)


See all BIRC5 antibodies
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Published Data
CBMAB-1576-YC-1
CBMAB-1576-YC in IHC
Immunohistochemical analysis of BIRC5 in mouse uterus (n = 4). LE, luminal epithelium; GE, glandular epithelium; S, stroma. ...View More
Cho, M., Lee, O. H., Chang, E. M., Lee, S., Moon, S., Lee, J., ... & Choi, Y. (2020). BIRC5 expression is regulated in uterine epithelium during the estrous cycle. Genes, 11(3), 282.
CBMAB-1576-YC-2
CBMAB-1576-YC in WB
Binding of antibody (60.11) used in imaging flow cytometry to full-length survivin protein and to survivin vaccine peptide aa53-67/M57 and wild type peptide aa53-67. ...View More
Galbo Jr, P. M., Ciesielski, M. J., Figel, S., Maguire, O., Qiu, J., Wiltsie, L., ... & Fenstermaker, R. A. (2017). Circulating CD9+/GFAP+/survivin+ exosomes in malignant glioma patients following survivin vaccination. Oncotarget, 8(70), 114722.
CBMAB-1576-YC-3
CBMAB-1576-YC in FC
Acetylcholine receptor alpha peptide binding to survivin positive cells in myasthenics by five color FACS analysis.
A) FITC labeled AChR-alpha subunit peptide binding to total myasthenic PBMC (0.44%), B) Excess unlabeled AChR-alpha subunit peptide followed by competitive binding of FITC-AChR peptide (0.03%). C) Control PBMC, subject number 19, (SVN+/CD38+/CD138+/CD20+ gated sub-population) showing FITC-AChR-alpha subunit peptide binding (0.33% of gated cells). D-F; Myasthenic patient PBMC (SVN+/CD38+/CD138+/CD20+ gated sub-population) showing FITC-AChR-alpha subunit peptide binding (1.55% (pt#10), 6.54% (pt#15) and 2.50% (pt#14) of total gated cells; n = 3). ...View More
Kusner, L. L., Ciesielski, M. J., Marx, A., Kaminski, H. J., & Fenstermaker, R. A. (2014). Survivin as a potential mediator to support autoreactive cell survival in myasthenia gravis: a human and animal model study. PloS one, 9(7), e102231.
CBMAB-1576-YC-4
CBMAB-1576-YC in IF
(A-F) Immunofluorescence for survivin (red) in macroscopically and microscopically arthritic cartilage (A, C, E) and adult non/moderate arthritic cartilage (paraffin-embedded tissue sections) (B, D, F). Chondrocytes were stained with 4,6-diamidino-2-phenylindole (blue) in the identical positions. Original magnifications: 200 × (A-D) and 400 × (E, F). ...View More
Lechler, P., Balakrishnan, S., Schaumburger, J., Grässel, S., Baier, C., Grifka, J., ... & Renkawitz, T. (2011). The oncofetal gene survivin is re-expressed in osteoarthritis and is required for chondrocyte proliferation in vitro. BMC musculoskeletal disorders, 12, 1-8.
CBMAB-1576-YC-5
CBMAB-1576-YC in WB
Western Blot Analysis of exosomal Survivin in untreated PCa plasmas.
A. Antibodies for Survivin and Lamp1 were used for Western blotting of control patient-purified exosomal protein. B. Both Survivin and Lamp1 antibodies were detected in the Western Blotting of exosomes-derived from Gleason 6 and Gleason 9. ...View More
Khan, S., Jutzy, J. M., Valenzuela, M. M. A., Turay, D., Aspe, J. R., Ashok, A., ... & Wall, N. R. (2012). Plasma-derived exosomal survivin, a plausible biomarker for early detection of prostate cancer.
CBMAB-1576-YC-6
CBMAB-1576-YC in IHC and Immunoblot
Survivin expression in human chondrosarcoma. Immunohistochemistry and immunoblot for survivin (red staining) from human chondrosarcoma specimens. A: Low-power image of human high-grade chondrosarcoma displays strong cellular expression of survivin protein. B: High-power magnification reveals the predominantly cytoplasmic staining, although strong nuclear signals are detectable. C and D: Other specimen of a grade III chondrosarcoma stained with monoclonal antibody, shows a similar pattern of staining. E: Strong survivin signal in a tumor cell displaying a mitotic figure (arrow). F: To verify the expression of survivin in human chondrosarcoma, immunoblots were performed from 3 high grade chondrosarcoma lysates. ...View More
Lechler, P., Renkawitz, T., Campean, V., Balakrishnan, S., Tingart, M., Grifka, J., & Schaumburger, J. (2011). The antiapoptotic gene survivin is highly expressed in human chondrosarcoma and promotes drug resistance in chondrosarcoma cells in vitro. BMC cancer, 11, 1-14.
CBMAB-1576-YC-7
CBMAB-1576-YC in IF
Survivin subcellular localization in human chondrosarcoma cells in vitro. Immunofluorescence localization of survivin using chondrosarcoma cells (SW1353) cultured on glass slides (A,D,G) and 4,6-diamidino-2-phenylindole-staining (DAPI) of the identical positions (B,E,H). Overlay of both stainings (C,F,I). A-C: The top row clearly shows the heterogeneous subcellular distribution from predominant cytoplasmic (lower cell) in the majority of the cell population to mixed cytoplasmic-nuclear in a smaller fraction of cells (upper cell). D-F: In a premitotic cell, survivin localizes to the mitotic spindle apparatus (arrow). Of note, here survivin signal appears stronger compared to the surrounding non-mitotic cells. G-I: In late telophase the mid-body (arrow) stains positive for survivin protein. Original magnifications: 400× (A-I) ...View More
Lechler, P., Renkawitz, T., Campean, V., Balakrishnan, S., Tingart, M., Grifka, J., & Schaumburger, J. (2011). The antiapoptotic gene survivin is highly expressed in human chondrosarcoma and promotes drug resistance in chondrosarcoma cells in vitro. BMC cancer, 11, 1-14.

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
60.11
Antibody Isotype
IgG2a
Application
FC, IH, WB, IF, E

Basic Information

Immunogen
Amino acids 27-67.
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG2a
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
IF(ICC)1:50-1:200
WB1:1,000
IHC1:50-1:200
FC1 µg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS
Preservative
0.1% sodium azide
Concentration
1 mg/ml
Purity
>95%, as determined by SDS-PAGE analysis
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Baculoviral IAP Repeat Containing 5
Introduction
BIRC5 is a member of the inhibitor of apoptosis (IAP) gene family which encode negative regulatory proteins that prevent apoptotic cell death. IAP family members usually contain multiple baculovirus IAP repeat (BIR) domains, but this gene encodes proteins with only a single BIR domain. The expression of BIRC5 is high during fetal development and in most tumors, yet low in adult tissues.
Entrez Gene ID
Human332
Mouse11799
Rat64041
UniProt ID
HumanO15392
MouseO70201
RatQ9JHY7
Alternative Names
API4; EPR-1
Function
Multitasking protein that has dual roles in promoting cell proliferation and preventing apoptosis (PubMed:9859993, PubMed:21364656, PubMed:20627126, PubMed:25778398, PubMed:28218735).
Component of a chromosome passage protein complex (CPC) which is essential for chromosome alignment and segregation during mitosis and cytokinesis (PubMed:16322459).
Acts as an important regulator of the localization of this complex; directs CPC movement to different locations from the inner centromere during prometaphase to midbody during cytokinesis and participates in the organization of the center spindle by associating with polymerized microtubules (PubMed:20826784).
Involved in the recruitment of CPC to centromeres during early mitosis via association with histone H3 phosphorylated at 'Thr-3' (H3pT3) during mitosis (PubMed:20929775).
The complex with RAN plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules (PubMed:18591255).
May counteract a default induction of apoptosis in G2/M phase (PubMed:9859993).
The acetylated form represses STAT3 transactivation of target gene promoters (PubMed:20826784).
May play a role in neoplasia (PubMed:10626797).
Inhibitor of CASP3 and CASP7 (PubMed:21536684).
Essential for the maintenance of mitochondrial integrity and function (PubMed:25778398).
Isoform 2 and isoform 3 do not appear to play vital roles in mitosis (PubMed:12773388, PubMed:16291752).
Isoform 3 shows a marked reduction in its anti-apoptotic effects when compared with the displayed wild-type isoform (PubMed:10626797).
Biological Process
Cell division Source: UniProtKB
Chromosome segregation Source: UniProtKB-KW
Cytokine-mediated signaling pathway Source: Reactome
Establishment of chromosome localization Source: UniProtKB
G2/M transition of mitotic cell cycle Source: UniProtKB
Mitotic cell cycle Source: UniProtKB
Mitotic cytokinesis Source: UniProtKB
Mitotic spindle assembly checkpoint Source: UniProtKB
Mitotic spindle organization Source: Reactome
Negative regulation of apoptotic process Source: UniProtKB
Negative regulation of cysteine-type endopeptidase activity involved in apoptotic process Source: UniProtKB
Negative regulation of transcription, DNA-templated Source: UniProtKB
Positive regulation of cell population proliferation Source: UniProtKB
Positive regulation of exit from mitosis Source: UniProtKB
Positive regulation of mitotic cell cycle Source: UniProtKB
Protein-containing complex localization Source: UniProtKB
Protein phosphorylation Source: UniProtKB
Regulation of apoptotic process Source: Reactome
Regulation of cell cycle Source: GO_Central
Sensory perception of sound Source: UniProtKB
Cellular Location
Spindle; Cytoplasm; Nucleus; Chromosome; Centromere; Kinetochore; Midbody. Localizes at the centromeres from prophase to metaphase, at the spindle midzone during anaphase and a the midbody during telophase and cytokinesis. Accumulates in the nucleus upon treatment with leptomycin B (LMB), a XPO1/CRM1 nuclear export inhibitor (By similarity). Localizes on chromosome arms and inner centromeres from prophase through metaphase. Localizes to kinetochores in metaphase, distributes to the midzone microtubules in anaphase and at telophase, localizes exclusively to the midbody (PubMed:11084331). Colocalizes with AURKB at mitotic chromosomes (PubMed:14610074). Acetylation at Lys-129 directs its localization to the nucleus by enhancing homodimerization and thereby inhibiting XPO1/CRM1-mediated nuclear export (PubMed:20826784).
PTM
Ubiquitinated by the Cul9-RING ubiquitin-protein ligase complex, leading to its degradation. Ubiquitination is required for centrosomal targeting.
In vitro phosphorylation at Thr-117 by AURKB prevents interaction with INCENP and localization to mitotic chromosomes (PubMed:14610074). Phosphorylation at Thr-48 by CK2 is critical for its mitotic and anti-apoptotic activities (PubMed:21252625). Phosphorylation at Thr-34 by CDK15 is critical for its anti-apoptotic activity (PubMed:24866247). Phosphorylation at Ser-20 by AURKC is critical for regulation of proper chromosome alignment and segregation, and possibly cytokinesis.
Acetylation at Lys-129 by CBP results in its homodimerization, while deacetylation promotes the formation of monomers which heterodimerize with XPO1/CRM1 which facilitates its nuclear export. The acetylated form represses STAT3 transactivation. The dynamic equilibrium between its acetylation and deacetylation at Lys-129 determines its interaction with XPO1/CRM1, its subsequent subcellular localization, and its ability to inhibit STAT3 transactivation.
More Infomation

He, D., Huang, K., & Liang, Z. (2021). Prognostic value of baculoviral IAP repeat containing 5 expression as a new biomarker in lung adenocarcinoma: a meta-analysis. Expert Review of Molecular Diagnostics, 1-9.

Mamoor, S. (2021). Over-expression of baculoviral IAP repeat containing 5 in human endometrial cancer.

Pahlavan, Y., & Khabbazi, A. (2021). Baculoviral inhibitor of apoptosis family of proteins repeat-containing 5 gene methylation status in peripheral blood mononuclear cells and plasma survivin levels in patients with Behçet’s disease. Archives of Rheumatology, 36(2).

Basha, R., Tran, N., & Ibarra-Aleman, V. (2021). Association of baculoviral inhibitor of apoptosis repeat-containing 5 in the survival of clear cell renal cell carcinoma patients: In silico analysis.

Li, Y., Zhao, Z. G., Luo, Y., Cui, H., Wang, H. Y., Jia, Y. F., & Gao, Y. T. (2020). Dual targeting of Polo-like kinase 1 and baculoviral inhibitor of apoptosis repeat-containing 5 in TP53-mutated hepatocellular carcinoma. World Journal of Gastroenterology, 26(32), 4786.

Zou, J., Liao, X., Zhang, J., & Wang, L. (2019). Dysregulation of miR-195-5p/-218-5p/BIRC5 axis predicts a poor prognosis in patients with gastric cancer. Journal of biological regulators and homeostatic agents, 33(5), 1377-1385.

Sušac, I., Ozretić, P., Gregorić, M., Levačić Cvok, M., Sabol, M., Levanat, S., ... & Musani, V. (2019). Polymorphisms in Survivin (BIRC5 Gene) are associated with age of onset in breast cancer patients. Journal of oncology, 2019.

Zhao, H., Zhang, X., Han, Z., Wang, Z., & Wang, Y. (2018). Plasma anti‐BIRC 5 IgG may be a useful marker for evaluating the prognosis of nonsmall cell lung cancer. FEBS Open Bio, 8(5), 829-835.

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For research use only. Not intended for any clinical use.

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