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Mouse Anti-BECN1 Recombinant Antibody (CB162A) (CBMAB-CA162LY)


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Summary

Host Animal
Mouse
Specificity
Human
Clone
CB162A
Antibody Isotype
IgG1
Application
IHC-P

Basic Information

Immunogen
Synthetic peptide derived from Beclin-1 at 110-190 aa.
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
IHC-P1:100-1:200

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 50% Glycerol, 0.5% BSA
Preservative
0.02% sodium azide
Concentration
1 mg/ml
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Beclin 1
Introduction
This gene encodes a protein that regulates autophagy, a catabolic process of degradation induced by starvation. The encoded protein is a component of the phosphatidylinositol-3-kinase (PI3K) complex which mediates vesicle-trafficking processes. This protein is thought to play a role in multiple cellular processes, including tumorigenesis, neurodegeneration and apoptosis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]
Entrez Gene ID
Human8678
Mouse56208
Rat114558
UniProt ID
HumanQ14457
MouseO88597
RatQ91XJ1
Alternative Names
Beclin 1; Coiled-Coil Myosin-Like BCL2-Interacting Protein; Beclin 1, Autophagy Related; Beclin 1 (Coiled-Coil, Moesin-Like BCL2 Interacting Protein); Beclin 1 (Coiled-Coil, Moesin-Like BCL2-Interacting Protein); ATG6 Autophagy Related 6 Homolog (S. Cerevisiae); Testis Secretory Sperm-Binding Protein Li 215e; ATG6 Autophagy Related 6 Homolog;
Function
Plays a central role in autophagy (PubMed:23184933, PubMed:28445460).
Acts as core subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate; different complex forms are believed to play a role in multiple membrane trafficking pathways: PI3KC3-C1 is involved in initiation of autophagosomes and PI3KC3-C2 in maturation of autophagosomes and endocytosis. Involved in regulation of degradative endocytic trafficking and required for the abcission step in cytokinesis, probably in the context of PI3KC3-C2 (PubMed:20643123, PubMed:20208530, PubMed:26783301).
Essential for the formation of PI3KC3-C2 but not PI3KC3-C1 PI3K complex forms. Involved in endocytosis (PubMed:25275521).
Protects against infection by a neurovirulent strain of Sindbis virus (PubMed:9765397).
May play a role in antiviral host defense.
Beclin-1-C 35 kDa localized to mitochondria can promote apoptosis; it induces the mitochondrial translocation of BAX and the release of proapoptotic factors.
Biological Process
Aging Source: Ensembl
Amyloid-beta metabolic process Source: Ensembl
Apoptotic process Source: UniProtKB-KW
Autophagosome assembly Source: UniProtKB
Autophagy Source: CAFA
Autophagy of mitochondrion Source: ParkinsonsUK-UCL
Cell division Source: UniProtKB-KW
Cellular defense response Source: ProtInc
Cellular response to aluminum ion Source: Ensembl
Cellular response to amino acid starvation Source: Ensembl
Cellular response to copper ion Source: Ensembl
Cellular response to epidermal growth factor stimulus Source: Ensembl
Cellular response to glucose starvation Source: UniProtKB
Cellular response to hydrogen peroxide Source: Ensembl
Cellular response to nitrogen starvation Source: GO_Central
Defense response to virus Source: UniProtKB-KW
Early endosome to late endosome transport Source: UniProtKB
Engulfment of apoptotic cell Source: Ensembl
Late endosome to vacuole transport Source: GO_Central
Lysosome organization Source: Ensembl
Macroautophagy Source: UniProtKB
Mitophagy Source: ParkinsonsUK-UCL
Mitotic metaphase plate congression Source: CACAO
Negative regulation of apoptotic process Source: ProtInc
Negative regulation of autophagosome assembly Source: Ensembl
Negative regulation of cell death Source: MGI
Negative regulation of cell population proliferation Source: Ensembl
Negative regulation of lysosome organization Source: Ensembl
Negative regulation of reactive oxygen species metabolic process Source: Ensembl
Neuron development Source: Ensembl
Positive regulation of attachment of mitotic spindle microtubules to kinetochore Source: CACAO
Positive regulation of autophagosome assembly Source: Ensembl
Positive regulation of cardiac muscle hypertrophy Source: Ensembl
Positive regulation of intrinsic apoptotic signaling pathway Source: UniProtKB
Positive regulation of phosphatidylinositol 3-kinase signaling Source: ParkinsonsUK-UCL
Protein deubiquitination Source: Reactome
Receptor catabolic process Source: UniProtKB
Regulation of catalytic activity Source: Ensembl
Regulation of cytokinesis Source: UniProtKB
Response to drug Source: Ensembl
Response to hypoxia Source: Ensembl
Response to iron(II) ion Source: Ensembl
Response to lead ion Source: Ensembl
Response to mitochondrial depolarisation Source: BHF-UCL
Response to vitamin E Source: Ensembl
Viral process Source: UniProtKB-KW
Cellular Location
Cytoplasm; Endoplasmic reticulum membrane; Mitochondrion membrane; Trans-Golgi network membrane; Endosome membrane; Endosome; Autophagosome. Interaction with ATG14 promotes translocation to autophagosomes. Expressed in dendrites and cell bodies of cerebellar Purkinje cells (By similarity).
Beclin-1-C 35 kDa: Cytoplasm; Mitochondrion; Nucleus
Beclin-1-C 37 kDa: Mitochondrion
PTM
Phosphorylation at Thr-119 by DAPK1 reduces its interaction with BCL2 and BCL2L1 and promotes induction of autophagy (PubMed:19180116). In response to autophagic stimuli, phosphorylated at serine residues by AMPK in an ATG14-dependent manner, and this phosphorylation is critical for maximally efficient autophagy (PubMed:23878393).
Polyubiquitinated by NEDD4, both with 'Lys-11'- and 'Lys-63'-linkages (PubMed:21936852). 'Lys-11'-linked polyubiquitination leads to degradation and is enhanced when the stabilizing interaction partner VPS34 is depleted (PubMed:21936852). Deubiquitinated by USP10 and USP13, leading to stabilize the PIK3C3/VPS34-containing complexes (PubMed:21962518). Polyubiquitinated at Lys-402 with 'Lys-48'-linkages (PubMed:28445460). 'Lys-48'-linked polyubiquitination of Lys-402 leads to degradation (PubMed:28445460). Deubiquitinated by ATXN3, leading to stabilization (PubMed:28445460).
Proteolytically processed by caspases including CASP8 and CASP3; the C-terminal fragments lack autophagy-inducing capacity and are proposed to induce apoptosis. Thus the cleavage is proposed to be an determinant to switch from autophagy to apoptosis pathways affecting cellular homeostasis including viral infections and survival of tumor cells.
More Infomation

Li, X., Yang, K. B., Chen, W., Mai, J., Wu, X. Q., Sun, T., ... & Zhu, X. F. (2021). CUL3 (cullin 3)-mediated ubiquitination and degradation of BECN1 (beclin 1) inhibit autophagy and promote tumor progression. Autophagy, 1-18.

Tan, P., He, L., Xing, C., Mao, J., Yu, X., Zhu, M., ... & Wang, R. F. (2020). Myeloid loss of Beclin 1 promotes PD-L1 hi precursor B cell lymphoma development. The Journal of clinical investigation, 129(12), 5261-5277.

Vega-Rubín-de-Celis, S. (2020). The role of Beclin 1-dependent autophagy in cancer. Biology, 9(1), 4.

Zheng, H. C., Zhao, S., Xue, H., Zhao, E. H., Jiang, H. M., & Hao, C. L. (2020). The Roles of Beclin 1 Expression in Gastric Cancer: A Marker for Carcinogenesis, Aggressive Behaviors and Favorable Prognosis, and a Target of Gene Therapy. Frontiers in Oncology, 10, 2897.

Menon, M. B., & Dhamija, S. (2018). Beclin 1 phosphorylation–at the center of autophagy regulation. Frontiers in cell and developmental biology, 6, 137.

Fernández, Á. F., Sebti, S., Wei, Y., Zou, Z., Shi, M., McMillan, K. L., ... & Levine, B. (2018). Disruption of the beclin 1–BCL2 autophagy regulatory complex promotes longevity in mice. Nature, 558(7708), 136-140.

Minamoto, T., Nakayama, K., Nakamura, K., Katagiri, H., Sultana, R., Ishibashi, T., ... & Kyo, S. (2018). Loss of beclin 1 expression in ovarian cancer: A potential biomarker for predicting unfavorable outcomes. Oncology letters, 15(1), 1170-1176.

Singh, N. P., Miranda, K., Singh, U. P., Nagarkatti, P., & Nagarkatti, M. (2018). Diethylstilbestrol (DES) induces autophagy in thymocytes by regulating Beclin-1 expression through epigenetic modulation. Toxicology, 410, 49-58.

Chang, C. J., Lin, J. F., Hsiao, C. Y., Chang, H. H., Li, H. J., Chang, H. H., ... & Hung, C. F. (2017). Lutein induces autophagy via beclin-1 upregulation in IEC-6 rat intestinal epithelial cells. The American journal of Chinese medicine, 45(06), 1273-1291.

Ranaghan, M. J., Durney, M. A., Mesleh, M. F., McCarren, P. R., Garvie, C. W., Daniels, D. S., ... & Perez, J. R. (2017). The autophagy-related Beclin-1 protein requires the coiled-coil and BARA domains to form a homodimer with submicromolar affinity. Biochemistry, 56(51), 6639-6651.

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For research use only. Not intended for any clinical use.

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