Mouse Anti-BDH2 Recombinant Antibody (CBYY-1999) (CBMAB-2348-YY)

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Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

CBYY-1999

Antibody Isotype

IgG2a, κ

Application

WB, IHC, IF, IP

Basic Information

Immunogen

Amino acids 64-114 mapping near the C-terminus of DHRS6 of human origin.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name

3-Hydroxybutyrate Dehydrogenase 2

Introduction

Dehydrogenase that mediates the formation of 2,5-dihydroxybenzoic acid (2,5-DHBA), a siderophore that shares structural similarities with bacterial enterobactin and associates with LCN2, thereby playing a key role in iron homeostasis and transport. Also acts as a 3-hydroxybutyrate dehydrogenase (By similarity).

Entrez Gene ID

56898

UniProt ID

Q9BUT1

Alternative Names

3-Hydroxybutyrate Dehydrogenase 2; Short Chain Dehydrogenase/Reductase Family 15C Member 1; Dehydrogenase/Reductase SDR Family Member 6; 3-Hydroxybutyrate Dehydrogenase, Type 2; R-Beta-Hydroxybutyrate Dehydrogenase; Oxidoreductase UCPA; SDR15C1; DHRS6; Short Chain Dehydrogenase/Reductase Family 15C, Member 1;

Function

Dehydrogenase that mediates the formation of 2,5-dihydroxybenzoic acid (2,5-DHBA), a siderophore that shares structural similarities with bacterial enterobactin and associates with LCN2, thereby playing a key role in iron assimilation and homeostasis. Plays a role in susceptibility to bacterial infection by providing an assimilable source of iron that is exploited by pathogenic bacteria (By similarity).
Also acts as a 3-hydroxybutyrate dehydrogenase (PubMed:16380372).

Biological Process

Epithelial cell differentiation Source: UniProtKB
Fatty acid beta-oxidation Source: HGNC-UCL
Heme metabolic process Source: UniProtKB
Iron ion homeostasis Source: UniProtKB
Ketone body biosynthetic process Source: Reactome
Siderophore biosynthetic process Source: UniProtKB

Cellular Location

Cytoplasm

More Infomation

References

Kwiatkowski, S. P., Bozko, M., Zarod, M., Witecka, A., Jagielski, A. K., & Drozak, J. (2021). The Mammalian Cytosolic Type 2 (R)-β-hydroxybutyrate Dehydrogenase (BDH2) is 4-oxo-L-proline Reductase (EC1. 1.1. 104). bioRxiv.

Fu, Y., Liu, F., Cao, S., Zhang, J., Wang, H., Wu, B., ... & Bao, S. (2021). Bdh2 Deficiency Promotes Endoderm-Biased Early Differentiation of Mouse Embryonic Stem Cells. Frontiers in cell and developmental biology, 9, 834.

Li, B., Liao, Z., Mo, Y., Zhao, W., Zhou, X., Xiao, X., ... & Zhang, Z. (2020). Inactivation of 3-hydroxybutyrate dehydrogenase type 2 promotes proliferation and metastasis of nasopharyngeal carcinoma by iron retention. British journal of cancer, 122(1), 102-110.

Cui, X. P., Wang, C. X., Wang, Z. Y., Li, J., Tan, Y. W., Gu, S. T., & Qin, C. K. (2019). LncRNA TP73-AS1 sponges miR-141-3p to promote the migration and invasion of pancreatic cancer cells through the up-regulation of BDH2. Bioscience reports, 39(3), BSR20181937.

Liang, H., Xiong, Z., Li, R., Hu, K., Cao, M., Yang, J., ... & Deng, M. (2019). BDH2 is downregulated in hepatocellular carcinoma and acts as a tumor suppressor regulating cell apoptosis and autophagy. Journal of Cancer, 10(16), 3735.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Associated Products

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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SDS
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Second antibody and isotype control

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