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Mouse Anti-BCL2L1 Recombinant Antibody (H5) (CBMAB-1025CQ)


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Published Data
Mouse Anti-BCL2L1 Recombinant Antibody (H5) (CBMAB-1025CQ) in Immunoblot
Immunoblots showing Bcl-2, Bcl-xL, and BAX levels in non-SnCs and SnCs exposed to 1.25 μM ABT-263 for 24 h. β-Actin was used as a loading control. Densitometric quantification of Bcl-2 and BAX was performed, and the BAX/Bcl-2 ratio (right panel) was calculated. ...View More
Ryu, W., Park, C. W., Kim, J., Lee, H., & Chung, H. (2023). The Bcl-2/Bcl-xL Inhibitor ABT-263 Attenuates Retinal Degeneration by Selectively Inducing Apoptosis in Senescent Retinal Pigment Epithelial Cells. Molecules and Cells, 46(7), 420.
Mouse Anti-BCL2L1 Recombinant Antibody (H5) (CBMAB-1025CQ) in WB
LoVo cells treated with HAs (3 mg/mL) for 24-48 h. Cells subjected to Western blotting to analyze signaling pathways related to mitochondria membrane potential. ...View More
Tsai, M. C., Chen, C. C., Tseng, T. H., Chang, Y. C., Lin, Y. J., Tsai, I. N., ... & Wang, C. J. (2023). Hibiscus Anthocyanins Extracts Induce Apoptosis by Activating AMP-Activated Protein Kinase in Human Colorectal Cancer Cells. Nutrients, 15(18), 3972.
Mouse Anti-BCL2L1 Recombinant Antibody (H5) (CBMAB-1025CQ) in WB
Western blot of Bcl-xL, in T-cells with acetate treatment (n = 3 independent experiments). ...View More
Lyu, J., Li, Z., Roberts, J. P., Qi, Y. A., & Xiong, J. (2023). The short-chain fatty acid acetate coordinates with CD30 to modulate T-cell survival. Molecular biology of the cell, 34(8), br11.
Mouse Anti-BCL2L1 Recombinant Antibody (H5) (CBMAB-1025CQ) in WB
Expression levels of Bcl-xL protein in indicated TCR-T cells after co-culturing with target cells for 24hours (left). Quantification of the relative abundances of Bcl-xL, normalized to β-actin protein (right). ...View More
Lah, S., Kim, S., Kang, I., Kim, H., Hupperetz, C., Jung, H., ... & Kim, C. H. (2023). Engineering second-generation TCR-T cells by site-specific integration of TRAF-binding motifs into the CD247 locus. Journal for Immunotherapy of Cancer, 11(4).

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
H5
Antibody Isotype
IgG1, κ
Application
WB, IP, ELISA, IHC-P, IF, FC

Basic Information

Immunogen
Amino acids 165-190 at the C-terminus of Bcl-xL of human origin.
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
IHC-P1:50-1:500
ELISA1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Purity
>95% as determined by analysis by SDS-PAGE
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
BCL2 Like 1
Introduction
The protein encoded by this gene belongs to the BCL-2 protein family. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. The proteins encoded by this gene are located at the outer mitochondrial membrane, and have been shown to regulate outer mitochondrial membrane channel (VDAC) opening. Diseases associated with BCL2L1 include Lung Cancer and Hepatocellular Carcinoma. Among its related pathways are RANK Signaling in Osteoclasts and Nucleotide-binding domain, leucine rich repeat containing receptor (NLR) signaling pathways.
Entrez Gene ID
598
UniProt ID
Q07817
Alternative Names
BCL2-Like 1; Apoptosis Regulator Bcl-X; BCL2L; BCLX; Protein Phosphatase 1; Regulatory Subunit 52; Protein Phosphatase 1; Regulatory Subunit 52; BCL-XL/S; Bcl2-L-1; PPP1R52; Bcl-XL; Bcl-XS; BCLXL; BCLXS; Bcl-X
Function
Potent inhibitor of cell death. Inhibits activation of caspases. Appears to regulate cell death by blocking the voltage-dependent anion channel (VDAC) by binding to it and preventing the release of the caspase activator, CYC1, from the mitochondrial membrane. Also acts as a regulator of G2 checkpoint and progression to cytokinesis during mitosis.
Isoform Bcl-X(L) also regulates presynaptic plasticity, including neurotransmitter release and recovery, number of axonal mitochondria as well as size and number of synaptic vesicle clusters. During synaptic stimulation, increases ATP availability from mitochondria through regulation of mitochondrial membrane ATP synthase F1F0 activity and regulates endocytic vesicle retrieval in hippocampal neurons through association with DMN1L and stimulation of its GTPase activity in synaptic vesicles. May attenuate inflammation impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release (PubMed:17418785).
Isoform Bcl-X(S) promotes apoptosis.
Biological Process
Apoptotic mitochondrial changes Source: ProtInc
Cytokine-mediated signaling pathway Source: Reactome
Defense response to virus Source: DIBU
Endocytosis Source: UniProtKB-KW
Extrinsic apoptotic signaling pathway in absence of ligand Source: GO_Central
Intrinsic apoptotic signaling pathway in response to DNA damage Source: GO_Central
MAPK cascade Source: Reactome
Mitotic cell cycle checkpoint Source: UniProtKB
Negative regulation of anoikis Source: UniProtKB
Negative regulation of apoptotic process Source: UniProtKB
Negative regulation of autophagy Source: UniProtKB
Negative regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway Source: UniProtKB
Negative regulation of execution phase of apoptosis Source: UniProtKB
Negative regulation of extrinsic apoptotic signaling pathway in absence of ligand Source: BHF-UCL
Negative regulation of extrinsic apoptotic signaling pathway via death domain receptors Source: MGI
Negative regulation of intrinsic apoptotic signaling pathway Source: BHF-UCL
Negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage Source: BHF-UCL
Negative regulation of protein localization to plasma membrane Source: BHF-UCL
Negative regulation of release of cytochrome c from mitochondria Source: BHF-UCL
Positive regulation of intrinsic apoptotic signaling pathway Source: Reactome
Regulation of cytokinesis Source: UniProtKB
Regulation of mitochondrial membrane permeability Source: HGNC-UCL
Regulation of mitochondrial membrane potential Source: HGNC-UCL
Release of cytochrome c from mitochondria Source: HGNC-UCL
Response to cytokine Source: MGI
Suppression by virus of host apoptotic process Source: MGI
Cellular Location
Isoform Bcl-X(L): Centrosome; Mitochondrion inner membrane; Mitochondrion outer membrane; Mitochondrion matrix; Cytosol; Nucleus membrane; Synaptic vesicle membrane. After neuronal stimulation, translocates from cytosol to synaptic vesicle and mitochondrion membrane in a calmodulin-dependent manner (By similarity). Localizes to the centrosome when phosphorylated at Ser-49.
Topology
Helical: 210-226 aa
PTM
Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity.
Phosphorylated on Ser-62 by CDK1. This phosphorylation is partial in normal mitotic cells, but complete in G2-arrested cells upon DNA-damage, thus promoting subsequent apoptosis probably by triggering caspases-mediated proteolysis. Phosphorylated by PLK3, leading to regulate the G2 checkpoint and progression to cytokinesis during mitosis. Phosphorylation at Ser-49 appears during the S phase and G2, disappears rapidly in early mitosis during prometaphase, metaphase and early anaphase, and re-appears during telophase and cytokinesis.
Ubiquitinated by RNF183 during prolonged ER stress, leading to degradation by the proteosome.
More Infomation

Yang, Z., An, Y., Wang, N., Dong, X., & Kang, H. (2020). LINC02595 promotes tumor progression in colorectal cancer by inhibiting miR‐203b‐3p activity and facilitating BCL2L1 expression. Journal of cellular physiology, 235(10), 7449-7464.

Lizárraga-Verdugo, E., Ruiz-García, E., Lopez-Camarillo, C., Bermúdez, M., Avendaño-Félix, M., Ramos-Payán, R., ... & Aguilar-Medina, M. (2020). Cell survival is regulated via SOX9/BCL2L1 Axis in HCT-116 colorectal cancer cell line. Journal of oncology, 2020.

O'Hara, S. P., Splinter, P. L., Trussoni, C. E., Guicciardi, M. E., Splinter, N. P., Al Suraih, M. S., ... & LaRusso, N. F. (2019). The transcription factor ETS1 promotes apoptosis resistance of senescent cholangiocytes by epigenetically up-regulating the apoptosis suppressor BCL2L1. Journal of Biological Chemistry, 294(49), 18698-18713.

Duan, S., Yu, S., Yuan, T., Yao, S., & Zhang, L. (2019). Exogenous let-7a-5p induces A549 lung cancer cell death through BCL2L1-mediated PI3Kγ signaling pathway. Frontiers in oncology, 9, 808.

Zhang, H., Zong, Y., Qiu, G., Jia, R., Xu, X., Wang, F., & Wu, D. (2018). Silencing Lin28 promotes apoptosis in colorectal cancer cells by upregulating let‑7c targeting of antiapoptotic BCL2L1. Molecular medicine reports, 17(4), 5143-5149.

Cai, J., Liu, T., Jiang, X., Guo, C., Liu, A., & Xiao, X. (2017). Downregulation of USP18 inhibits growth and induces apoptosis in hepatitis B virus-related hepatocellular carcinoma cells by suppressing BCL2L1. Experimental cell research, 358(2), 315-322.

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For research use only. Not intended for any clinical use.

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