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Mouse Anti-BAZ1B Recombinant Antibody (CBWJW-167) (CBMAB-B0004-WJ)


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Published Data
Mouse Anti-BAZ1B Recombinant Antibody (CBWJW-167) (CBMAB-B0004-WJ) in WB
Triptolide downregulates the WSTF and RPB1 signaling pathway without affecting TAB1/P38 signaling in a dose dependent manner in MV-4-11 (A) and THP-1 (B). ...View More
Kang, D., Liu, Y., Song, Y., Fang, B., Zhang, Q., & Hu, L. (2022). Triptolide shows high sensitivity and low toxicity against acute myeloid leukemia cell lines through inhibiting WSTF-RNAPII complex. Frontiers in Oncology, 12, 811850.
Mouse Anti-BAZ1B Recombinant Antibody (CBWJW-167) (CBMAB-B0004-WJ) in WB
Detection of full-length and IPA-generated truncated mRNAs by RT-PCR in normal B cells (CD5+B, BLCL) and CLL cells used in the western blot validations. ...View More
Lee, S. H., Singh, I., Tisdale, S., Abdel-Wahab, O., Leslie, C. S., & Mayr, C. (2018). Widespread intronic polyadenylation inactivates tumour suppressor genes in leukaemia. Nature, 561(7721), 127-131.

Summary

Host Animal
Mouse
Specificity
Mouse, Rat, Human
Clone
CBWJW-167
Antibody Isotype
IgG1, κ
Application
WB, IP, IF, ELISA, IHC-P

Basic Information

Immunogen
Amino acids 1-300 mapping at the N-terminus of WSTF of human origin.
Host Species
Mouse
Specificity
Mouse, Rat, Human
Antibody Isotype
IgG1, κ
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
ELISA1:100-1:1,000
IHC-P1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
bromodomain adjacent to zinc finger domain, 1B
Introduction
BAZ1B is a protein-coding gene encodes a member of the bromodomain protein family. This gene is deleted in Williams-Beuren syndrome, a developmental disorder caused by deletion of multiple genes at 7q11.23. Diseases associated with BAZ1B include Williams-Beuren Syndrome and Chromosomal Deletion Syndrome. An important paralog of this gene is BPTF.
Entrez Gene ID
Human9031
Mouse22385
Rat368002
UniProt ID
HumanQ9UIG0
MouseQ9Z277
RatG3V661
Alternative Names
WSTF; WBSCR9; WBSCR10
Function
Atypical tyrosine-protein kinase that plays a central role in chromatin remodeling and acts as a transcription regulator. Involved in DNA damage response by phosphorylating 'Tyr-142' of histone H2AX (H2AXY142ph). H2AXY142ph plays a central role in DNA repair and acts as a mark that distinguishes between apoptotic and repair responses to genotoxic stress. Essential component of the WICH complex, a chromatin remodeling complex that mobilizes nucleosomes and reconfigures irregular chromatin to a regular nucleosomal array structure. The WICH complex regulates the transcription of various genes, has a role in RNA polymerase I and RNA polymerase III transcription, mediates the histone H2AX phosphorylation at 'Tyr-142', and is involved in the maintenance of chromatin structures during DNA replication processes. In the complex, it mediates the recruitment of the WICH complex to replication foci during DNA replication.
Biological Process
Cellular response to DNA damage stimulus Source: UniProtKB
Chromatin assembly or disassembly Source: Ensembl
Chromatin remodeling Source: BHF-UCL
Double-strand break repair Source: Reactome
Histone phosphorylation Source: UniProtKB
Positive regulation of gene expression, epigenetic Source: Reactome
Regulation of transcription by RNA polymerase II Source: BHF-UCL
Cellular Location
Nucleus. Accumulates in pericentromeric heterochromatin during replication. Targeted to replication foci throughout S phase via its association with PCNA.
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For research use only. Not intended for any clinical use.

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