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Rabbit Anti-BATF Recombinant Antibody (EG353) (CBMAB-EN390-LY)


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Summary

Host Animal
Rabbit
Specificity
Human
Clone
EG353
Antibody Isotype
IgG
Application
WB

Basic Information

Immunogen
Recombinant fragment of human BATF.
Specificity
Human
Antibody Isotype
IgG
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:500-1:3,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS
Preservative
None
Concentration
1 mg/ml
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Basic Leucine Zipper ATF-Like Transcription Factor
Introduction
The protein encoded by this gene is a nuclear basic leucine zipper protein that belongs to the AP-1/ATF superfamily of transcription factors. The leucine zipper of this protein mediates dimerization with members of the Jun family of proteins. This protein is thought to be a negative regulator of AP-1/ATF transcriptional events. [provided by RefSeq, Jul 2008]
Entrez Gene ID
Human10538
Mouse53314
UniProt ID
HumanQ16520
MouseO35284
Alternative Names
Basic Leucine Zipper ATF-Like Transcription Factor; Basic Leucine Zipper Transcription Factor, ATF-Like; B-Cell-Activating Transcription Factor; Activating Transcription Factor B; SF-HT-Activated Gene 2 Protein; B-ATF;
Function
AP-1 family transcription factor that controls the differentiation of lineage-specific cells in the immune system: specifically mediates the differentiation of T-helper 17 cells (Th17), follicular T-helper cells (TfH), CD8+ dendritic cells and class-switch recombination (CSR) in B-cells. Acts via the formation of a heterodimer with JUNB that recognizes and binds DNA sequence 5'-TGA[CG]TCA-3'. The BATF-JUNB heterodimer also forms a complex with IRF4 (or IRF8) in immune cells, leading to recognition of AICE sequence (5'-TGAnTCA/GAAA-3'), an immune-specific regulatory element, followed by cooperative binding of BATF and IRF4 (or IRF8) and activation of genes. Controls differentiation of T-helper cells producing interleukin-17 (Th17 cells) by binding to Th17-associated gene promoters: regulates expression of the transcription factor RORC itself and RORC target genes such as IL17 (IL17A or IL17B). Also involved in differentiation of follicular T-helper cells (TfH) by directing expression of BCL6 and MAF. In B-cells, involved in class-switch recombination (CSR) by controlling the expression of both AICDA and of germline transcripts of the intervening heavy-chain region and constant heavy-chain region (I(H)-C(H)). Following infection, can participate in CD8+ dendritic cell differentiation via interaction with IRF4 and IRF8 to mediate cooperative gene activation. Regulates effector CD8+ T-cell differentiation by regulating expression of SIRT1. Following DNA damage, part of a differentiation checkpoint that limits self-renewal of hematopoietic stem cells (HSCs): up-regulated by STAT3, leading to differentiation of HSCs, thereby restricting self-renewal of HSCs (By similarity).
Biological Process
Cellular response to DNA damage stimulus Source: UniProtKB
Cytokine-mediated signaling pathway Source: Reactome
Defense response to protozoan Source: UniProtKB
DNA damage response, signal transduction by p53 class mediator Source: UniProtKB
Hematopoietic stem cell differentiation Source: UniProtKB
Isotype switching Source: UniProtKB
Lymphoid progenitor cell differentiation Source: UniProtKB
Myeloid dendritic cell differentiation Source: UniProtKB
Positive regulation of cytokine production Source: UniProtKB
Regulation of transcription by RNA polymerase II Source: GO_Central
T-helper 17 cell differentiation Source: UniProtKB
T-helper 17 cell lineage commitment Source: UniProtKB
T-helper 2 cell differentiation Source: UniProtKB
Cellular Location
Cytoplasm; Nucleus. Present in the nucleus and cytoplasm, but shows increased nuclear translocation after activation of T-cells.
PTM
Phosphorylated on serine and threonine residues and at least one tyrosine residue. Phosphorylation at Ser-43 inhibit DNA binding activity and transforms it as a negative regulator of AP-1 mediated transcription (By similarity).
Phosphorylated.
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For research use only. Not intended for any clinical use.

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