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Rabbit Anti-BAG1 Recombinant Antibody (RM310) (CBMAB-BR050LY)


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Summary

Host Animal
Rabbit
Specificity
Human
Clone
RM310
Antibody Isotype
IgG
Application
IHC, WB, ICC

Basic Information

Immunogen
A peptide corresponding to residues near the N-terminus of human BAG-1L.
Specificity
Human
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:1,000-1:2,000
IF(ICC)1:200-1:1,000
IHC1:500-1:2,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 1% BSA, 50% glycerol
Preservative
0.09% sodium azide
Concentration
Batch dependent
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
BCL2 Associated Athanogene 1
Introduction
The oncogene BCL2 is a membrane protein that blocks a step in a pathway leading to apoptosis or programmed cell death. The protein encoded by this gene binds to BCL2 and is referred to as BCL2-associated athanogene. It enhances the anti-apoptotic effects of BCL2 and represents a link between growth factor receptors and anti-apoptotic mechanisms. Multiple protein isoforms are encoded by this mRNA through the use of a non-AUG (CUG) initiation codon, and three alternative downstream AUG initiation codons. A related pseudogene has been defined on chromosome X. [provided by RefSeq, Feb 2010]
Entrez Gene ID
573
UniProt ID
Q99933
Alternative Names
BAG family molecular chaperone regulator 1;BAG-1;Bcl-2-associated athanogene 1;BAG1;HAP;
Function
Co-chaperone for HSP70 and HSC70 chaperone proteins. Acts as a nucleotide-exchange factor (NEF) promoting the release of ADP from the HSP70 and HSC70 proteins thereby triggering client/substrate protein release. Nucleotide release is mediated via its binding to the nucleotide-binding domain (NBD) of HSPA8/HSC70 where as the substrate release is mediated via its binding to the substrate-binding domain (SBD) of HSPA8/HSC70 (PubMed:27474739, PubMed:9873016, PubMed:24318877).
Inhibits the pro-apoptotic function of PPP1R15A, and has anti-apoptotic activity (PubMed:12724406).
Markedly increases the anti-cell death function of BCL2 induced by various stimuli (PubMed:9305631).
Biological Process
Apoptotic process Source: UniProtKB-KW
Cell surface receptor signaling pathway Source: ProtInc
Chaperone cofactor-dependent protein refolding Source: UniProtKB
Negative regulation of apoptotic process Source: ProtInc
Protein stabilization Source: GO_Central
Regulation of cellular response to heat Source: Reactome
Cellular Location
Isoform 1: Cytoplasm; Nucleus. Isoform 1 localizes predominantly to the nucleus.
Isoform 2: Cytoplasm; Nucleus. Isoform 2 localizes to the cytoplasm and shuttles into the nucleus in response to heat shock.
Isoform 4: Cytoplasm; Nucleus. Isoform 4 localizes predominantly to the cytoplasm. The cellular background in which it is expressed can influence whether it resides primarily in the cytoplasm or is also found in the nucleus. In the presence of BCL2, localizes to intracellular membranes (what appears to be the nuclear envelope and perinuclear membranes) as well as punctate cytosolic structures suggestive of mitochondria.
PTM
Ubiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation.
More Infomation

Turk, M., Tatli, O., Alkan, H. F., Ozfiliz Kilbas, P., Alkurt, G., & Dinler Doganay, G. (2021). Co-Chaperone Bag-1 Plays a Role in the Autophagy-Dependent Cell Survival through Beclin 1 Interaction. Molecules, 26(4), 854.

Can, N. D., Basturk, E., Kizilboga, T., Akcay, I. M., Dingiloglu, B., Tatli, O., ... & Dinler Doganay, G. (2021). Interactome analysis of Bag-1 isoforms reveals novel interaction partners in endoplasmic reticulum-associated degradation. PloS one, 16(8), e0256640.

Luo, S., Hou, Y., Zhang, Y., Feng, L., Hunter, R. G., Yuan, P., ... & Du, J. (2021). Bag‐1 mediates glucocorticoid receptor trafficking to mitochondria after corticosterone stimulation: Potential role in regulating affective resilience. Journal of Neurochemistry, 158(2), 358-372.

Nguyen, P., Hess, K., Smulders, L., Le, D., Briseno, C., Chavez, C. M., & Nikolaidis, N. (2020). Origin and evolution of the human Bcl2-associated athanogene-1 (BAG-1). International journal of molecular sciences, 21(24), 9701.

Song, Y. K., Li, Z., Wang, Y., Qu, Y., Li, Q. S., Man, X. Y., ... & Hu, D. (2020). Inhibition of BAG‐1 induced SH‐SY5Y cell apoptosis without affecting Hsp70 expression. Journal of cellular biochemistry, 121(2), 1728-1735.

Kirschke, E., Roe-Zurz, Z., Noddings, C., & Agard, D. (2020). The interplay between Bag-1, Hsp70, and Hsp90 reveals that inhibiting Hsp70 rebinding is essential for Glucocorticoid Receptor activity. bioRxiv.

Papadakis, E. S., Reeves, T., Robson, N. H., Maishman, T., Packham, G., & Cutress, R. I. (2017). BAG-1 as a biomarker in early breast cancer prognosis: a systematic review with meta-analyses. British journal of cancer, 116(12), 1585-1594.

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For research use only. Not intended for any clinical use.

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