Mouse Anti-BACE2 Recombinant Antibody (F-12) (CBMAB-1185-CN)

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Datasheet

Summary

Host Animal

Mouse

Specificity

Human, Mouse, Rat

Clone

F-12

Antibody Isotype

IgG1, κ

Application

WB, IF, IP, ELISA

Basic Information

Immunogen

Amino acids 429-518 mapping near the C-terminus of BACE2 of human origin.

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name

Beta-Site APP-Cleaving Enzyme 2

Introduction

This gene encodes an integral membrane glycoprotein that functions as an aspartic protease. The encoded protein cleaves amyloid precursor protein into amyloid beta peptide, which is a critical step in the etiology of Alzheimer's disease and Down syndrome. The protein precursor is further processed into an active mature peptide. Responsible for the proteolytic processing of the amyloid precursor protein (APP). It has also been shown that it can cleave APP between residues 671 and 672.

Entrez Gene ID

25825

UniProt ID

Q9Y5Z0

Alternative Names

ASP1; BAE2; DRAP; AEPLC; ALP56; ASP21; CDA13; CEAP1

Function

Responsible for the proteolytic processing of the amyloid precursor protein (APP). Cleaves APP, between residues 690 and 691, leading to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated C-terminal fragment which is later released by gamma-secretase. It has also been shown that it can cleave APP between residues 671 and 672. Responsible also for the proteolytic processing of CLTRN in pancreatic beta cells (PubMed:21907142).

Biological Process

Amyloid-beta metabolic process Source: GO_Central
Astrocyte activation Source: Ensembl
Glucose homeostasis Source: UniProtKB
Membrane protein ectodomain proteolysis Source: UniProtKB
Negative regulation of amyloid precursor protein biosynthetic process Source: UniProtKB
Peptide hormone processing Source: UniProtKB
Proteolysis Source: UniProtKB

Cellular Location

Endoplasmic reticulum; Golgi apparatus; Endosome; Cell membrane

Topology

Extracellular: 21-473 aa
Helical: 474-494 aa
Cytoplasmic: 495-518 aa

PTM

Undergoes autoproteolytic cleavage.
Glycosylated.

More Infomation

References

Fujimoto, K., Yoshida, S., Tadano, G., Asada, N., Fuchino, K., Suzuki, S., ... & Kusakabe, K. I. (2021). Structure-Based Approaches to Improving Selectivity through Utilizing Explicit Water Molecules: Discovery of Selective β-Secretase (BACE1) Inhibitors over BACE2. Journal of Medicinal Chemistry, 64(6), 3075-3085.

He, F., Yu, J., Yang, J., Wang, S., Zhuang, A., Shi, H., ... & Jia, R. (2021). m6A RNA hypermethylation-induced BACE2 boosts intracellular calcium release and accelerates tumorigenesis of ocular melanoma. Molecular Therapy.

Yen, Y. C., Kammeyer, A. M., Tirlangi, J., Ghosh, A. K., & Mesecar, A. D. (2021). A Structure-Based Discovery Platform for BACE2 and the Development of Selective BACE Inhibitors. ACS Chemical Neuroscience, 12(4), 581-588.

Wang, Z., Xu, Q., Cai, F., Liu, X., Wu, Y., & Song, W. (2019). BACE2, a conditional β-secretase, contributes to Alzheimer’s disease pathogenesis. JCI insight, 4(1).

Liu, F., Zhang, Y., Liang, Z., Sun, Q., Liu, H., Zhao, J., ... & Sun, X. (2018). Cleavage of potassium channel Kv2. 1 by BACE2 reduces neuronal apoptosis. Molecular psychiatry, 23(7), 1542-1554.

Alcarraz-Vizán, G., Castaño, C., Visa, M., Montane, J., Servitja, J. M., & Novials, A. (2017). BACE2 suppression promotes β-cell survival and function in a model of type 2 diabetes induced by human islet amyloid polypeptide overexpression. Cellular and Molecular Life Sciences, 74(15), 2827-2838.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Associated Products

Related Products

Mouse Anti-BACE2 Recombinant Antibody (H-3)

Mouse Anti-BACE2 Recombinant Antibody (CBYY-1737)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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Documents

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SDS
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Second antibody and isotype control

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