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Mouse Anti-AVEN Recombinant Antibody (3G4) (CBMAB-A4207-YC)


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Published Data
Mouse Anti-AVEN Recombinant Antibody (3G4) in WB and IF
Aven Amino Acid Sequence and Expression Profiles.
(A) Underlined amino acids correspond to two peptides in the predicted sequence of Aven that were used to generate rabbit polyclonal anti-Aven antibodies.
(B) Northern blot analysis of multiple tissue blots.
(C) Northern blot analysis of poly(A)+ RNA isolated from the indicated cell lines.
(D) Cell lysates prepared from 7.5 × 105 HeLa cells/lane were separated by SDS-PAGE and immunoblotted with preimmune or postimmune antisera prepared from the N-terminal peptide (anti-Aven-a) or C-terminal peptide (anti-Aven-b).
(E) Immunoblot analysis was performed with anti-Aven-a antibody to detect endogenous Aven protein in fractionated HeLa cells. H = heavy, Lt = light, Cyto (see Experimental Procedures).
(F-H) Confocal microscopy of BHK cells transiently transfected with Aven plus Bcl-xL cDNAs and stained with anti-Aven-b plus rhodamine-conjugated secondary antibody (F) and anti-Bcl-xL antibody plus FITC-conjugated secondary antibody (G). Merging of the Aven image (red) with the Bcl-xL image (green) is shown in yellow (H). Controls verified specificity of the emission patterns (not shown). ...View More
Chau, B. N., Cheng, E. H. Y., Kerr, D. A., & Hardwick, J. M. (2000). Aven, a novel inhibitor of caspase activation, binds Bcl-xL and Apaf-1. Molecular cell, 6(1), 31-40.

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
3G4
Antibody Isotype
IgG2a
Application
WB, ELISA, IHC

Basic Information

Immunogen
Recombinant human PDCD12 (254-362 aa) purified from E. coli.
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG2a
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
IHC3 µg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.4
Preservative
None
Concentration
Batch dependent
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Apoptosis And Caspase Activation Inhibitor
Introduction
AVEN protects against apoptosis mediated by Apaf-1.
Entrez Gene ID
57099
UniProt ID
Q9NQS1
Alternative Names
Apoptosis And Caspase Activation Inhibitor; Cell Death Regulator Aven; Programmed Cell Death 12; Apoptosis, Caspase Activation Inhibitor; PDCD12;
Function
Protects against apoptosis mediated by Apaf-1.
Biological Process
Apoptotic process Source: UniProtKB-KW
Negative regulation of apoptotic process Source: UniProtKB
Negative regulation of G2/M transition of mitotic cell cycle Source: GO_Central
Cellular Location
Endomembrane system. Associated with intracellular membranes.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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