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Mouse Anti-ATP5PD Recombinant Antibody (7F9BG1) (CBMAB-A4070-YC)


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Published Data
Mouse Anti-ATP5PD Recombinant Antibody (7F9BG1) in WB
SDS-PAGE of mitochondrial fraction stained with antibodies against a-, b-, d-, e-, b-subunit, OSCP and VDAC.
Fujikawa, M., Sugawara, K., Tanabe, T., & Yoshida, M. (2015). Assembly of human mitochondrial ATP synthase through two separate intermediates, F1-c-ring and b-e-g complex. FEBS letters, 589(19), 2707-2712.
Mouse Anti-ATP5PD Recombinant Antibody (7F9BG1) in WB
SDS-PAGE of mitochondrial fraction stained with antibodies against a-, b-, d-, e-, b-subunit, OSCP and VDAC.
Fujikawa, M., Sugawara, K., Tanabe, T., & Yoshida, M. (2015). Assembly of human mitochondrial ATP synthase through two separate intermediates, F1-c-ring and b-e-g complex. FEBS letters, 589(19), 2707-2712.
Mouse Anti-ATP5PD Recombinant Antibody (7F9BG1) in WB
SDS-PAGE of mitochondrial fraction stained with antibodies against a-, b-, d-, e-, b-subunit, OSCP and VDAC.
Fujikawa, M., Sugawara, K., Tanabe, T., & Yoshida, M. (2015). Assembly of human mitochondrial ATP synthase through two separate intermediates, F1-c-ring and b-e-g complex. FEBS letters, 589(19), 2707-2712.

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat, Cattle
Clone
7F9BG1
Antibody Isotype
IgG2b, κ
Application
WB, FC, ICC, IF

Basic Information

Immunogen
Bovine Complex V.
Specificity
Human, Mouse, Rat, Cattle
Antibody Isotype
IgG2b, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1 µg/ml
IF(ICC)1-5 µg/ml
FC1 µg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
HEPES
Preservative
0.02% sodium azide
Concentration
1 mg/ml
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
ATP Synthase Peripheral Stalk Subunit D
Introduction
Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. It is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the
Entrez Gene ID
Human10476
Mouse71679
Rat641434
Cattle282710
Monkey700338
UniProt ID
HumanO75947
MouseQ9DCX2
RatP31399
CattleP13620
MonkeyA0A2K6K943
Alternative Names
ATP Synthase Peripheral Stalk Subunit D; ATP Synthase, H+ Transporting, Mitochondrial Fo Complex Subunit D; ATP5H; ATP Synthase, H+ Transporting, Mitochondrial F0 Complex, Subunit D; ATP Synthase, H+ Transporting, Mitochondrial Fo Complex, Subunit D; ATPa
Function
Mitochondrial membrane ATP synthase (F1F0 ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F1 - containing the extramembraneous catalytic core, and F0 - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F1 is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F0 domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha3beta3 subcomplex and subunit a/ATP6 static relative to the rotary elements.
Biological Process
ATP biosynthetic process Source: Reactome
ATP synthesis coupled proton transport Source: GO_Central
Cellular response to peptide Source: Ensembl
Cristae formation Source: Reactome
Mitochondrial ATP synthesis coupled proton transport Source: UniProtKB
Cellular Location
Mitochondrion; Mitochondrion inner membrane
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For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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