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Rabbit Anti-ATP5F1A Recombinant Antibody (555) (CBMAB-AP489LY)


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Summary

Host Animal
Rabbit
Specificity
Human, Mouse, Rat
Clone
555
Antibody Isotype
IgG
Application
WB, ICC, IF, IHC, FC

Basic Information

Immunogen
Human ATP5A1 between 100-300 amino acids.
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:300-1:5,000
IHC1:200-1:400
FC1:20-1:100
IF(ICC)1:50-1:200

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
TBS, pH7.4, 1%BSA, 40%Glycerol
Preservative
0.05% sodium azide
Concentration
1 mg/ml
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
ATP Synthase F1 Subunit Alpha
Introduction
This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, using an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the alpha subunit of the catalytic core. Alternatively spliced transcript variants encoding the different isoforms have been identified. Pseudogenes of this gene are located on chromosomes 9, 2, and 16. [provided by RefSeq, Mar 2012]
Entrez Gene ID
Human498
Mouse11946
Rat65262
UniProt ID
HumanP25705
MouseQ03265
RatP15999
Alternative Names
ATP Synthase F1 Subunit Alpha; ATP Synthase, H+ Transporting, Mitochondrial F1 Complex, Alpha Subunit 1, Cardiac Muscle; ATP5A1; ATP Synthase, H+ Transporting, Mitochondrial F1 Complex, Alpha Subunit, Isoform 2, Non-Cardiac Muscle-Like 2; ATP Synthase, H+ Transporting, Mitochondrial F1 Complex, Alpha Subunit, Isoform 1, Cardiac Muscle; ATP5AL2; ATP5A; ATPM; Epididymis Secretory Sperm Binding Protein Li 123m; Mitochondrial ATP Synthetase, Oligomycin-Resistant; ATP Synthase Subunit Alpha, Mitochondrial; ATP Synthase Alpha Chain, Mitochondrial;
Function
Mitochondrial membrane ATP synthase (F1F0 ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F1 - containing the extramembraneous catalytic core, and F0 - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F1 is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F1. Rotation of the central stalk against the surrounding alpha3beta3 subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits. Subunit alpha does not bear the catalytic high-affinity ATP-binding sites (By similarity).
Binds the bacterial siderophore enterobactin and can promote mitochondrial accumulation of enterobactin-derived iron ions (PubMed:30146159).
Biological Process
ATP biosynthetic process Source: CAFA
ATP synthesis coupled proton transport Source: GO_Central
Cristae formation Source: Reactome
Lipid metabolic process Source: UniProtKB
Mitochondrial ATP synthesis coupled proton transport Source: UniProtKB
Negative regulation of endothelial cell proliferation Source: UniProtKB
Positive regulation of blood vessel endothelial cell migration Source: CAFA
Cellular Location
Mitochondrion; Mitochondrion inner membrane; Cell membrane. Colocalizes with HRG on the cell surface of T-cells (PubMed:19285951).
Involvement in disease
Combined oxidative phosphorylation deficiency 22 (COXPD22): A mitochondrial disorder characterized by intrauterine growth retardation, microcephaly, hypotonia, pulmonary hypertension, failure to thrive, encephalopathy, and heart failure.
Mitochondrial complex V deficiency, nuclear type 4 (MC5DN4): A mitochondrial disorder with heterogeneous clinical manifestations including dysmorphic features, psychomotor retardation, hypotonia, growth retardation, cardiomyopathy, enlarged liver, hypoplastic kidneys and elevated lactate levels in urine, plasma and cerebrospinal fluid.
PTM
The N-terminus is blocked.
Acetylated on lysine residues. BLOC1S1 is required for acetylation.
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For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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