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Mouse Anti-ATF1 Recombinant Antibody (3E7) (CBMAB-A0555-LY)


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Summary

Host Animal
Mouse
Specificity
Human
Clone
3E7
Antibody Isotype
IgG1, κ
Application
WB, ELISA

Basic Information

Immunogen
ATF1 (AAH29619, 1 a.a. ~ 271 a.a) full-length recombinant protein with GST tag.
Specificity
Human
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.4
Preservative
None
Concentration
Batch dependent
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Activating Transcription Factor 1
Entrez Gene ID
466
UniProt ID
P18846
Alternative Names
EWS-ATF1; FUS/ATF-1; TREB36
Function
This protein binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Binds to the Tax-responsive element (TRE) of HTLV-I. Mediates PKA-induced stimulation of CRE-reporter genes. Represses the expression of FTH1 and other antioxidant detoxification genes. Triggers cell proliferation and transformation.
Biological Process
Cellular protein-containing complex assembly Source: Ensembl
Positive regulation of DNA replication Source: Ensembl
Positive regulation of neuron projection development Source: Ensembl
Positive regulation of transcription by RNA polymerase II Source: NTNU_SB
Regulation of transcription by RNA polymerase II Source: GO_Central
Response to cobalt ion Source: Ensembl
Response to organic cyclic compound Source: Ensembl
Cellular Location
Nucleus
Involvement in disease
Angiomatoid fibrous histiocytoma (AFH): The gene represented in this entry may be involved in disease pathogenesis. Chromosomal aberrations involving ATF1 are found in patients with angiomatoid fibrous histiocytoma. Translocation t(12;16)(q13;p11.2) with FUS generates a chimeric ATF1/FUS protein. Translocation t(12;22)(q13;q12) with EWSR1 generates a chimeric ATF1/EWSR1 protein. A distinct variant of malignant fibrous histiocytoma that typically occurs in children and adolescents and is manifest by nodular subcutaneous growth. Characteristic microscopic features include lobulated sheets of histiocyte-like cells intimately associated with areas of hemorrhage and cystic pseudovascular spaces, as well as a striking cuffing of inflammatory cells, mimicking a lymph node metastasis.
PTM
Phosphorylated at Ser-198 by HIPK2 in response to genotoxic stress. This phosphorylation promotes transcription repression of FTH1 and other antioxidant detoxification genes. The CDK3-mediated phosphorylation at Ser-63 promotes its transactivation and transcriptional activities. Phosphorylated at Ser-63 by RPS6KA4 and RPS6KA5 in response to mitogenic or stress stimuli.
More Infomation

Tabara, M., Shiraishi, K., Takii, R., Fujimoto, M., Nakai, A., & Matsuyama, H. (2021). Testicular localization of activating transcription factor 1 and its potential function during spermatogenesis. Biology of Reproduction.

Seneviratne, A., Han, Y., Wong, E., Walter, E. R., Jiang, L., Cave, L., ... & Boyle, J. J. (2020). Hematoma resolution in vivo is directed by activating transcription factor 1. Circulation research, 127(7), 928-944.

Ge, Y., Cave, L., Seneviratne, A., Carling, D., Mason, J. C., Haskard, D., & Boyle, J. J. (2020). Switching macrophage gene expression from inflammation-resolution to hemorrhage-resolution by redirection of activating transcription factor 1 (ATF1) binding by SMARCA4, BACH1 and histone H3K9 acetylation. Atherosclerosis, 315, e2.

Wu, L., Guo, Y., Cai, S., Kuang, L., Shen, Q., Wu, D., & Zhang, G. (2020). The zinc finger transcription factor ATF1 regulates aluminum tolerance in barley. Journal of Experimental Botany, 71(20), 6512-6523.

Cui, J., Yin, Z., Liu, G., Chen, X., Gao, X., Lu, H., ... & Lin, D. (2019). Activating transcription factor 1 promoted migration and invasion in lung cancer cells through regulating EGFR and MMP‐2. Molecular carcinogenesis, 58(10), 1919-1924.

Boyle, J., Seneviratne, A., Han, Y., Jiang, L., Walter, E., Cave, L., ... & Haskard, D. O. (2019). Vertebrate Hematoma Resolution Is Directed By Activating Transcription Factor 1 (Atf1) And Adenosine-Monophosphate-Activated-Protein-Kinase (Ampk). Atherosclerosis, 287, e246.

Boyle, J., Seneviratne, A., Tsao, A., Shaikh, A., Carling, D., Haskard, D. O., & Cave, L. (2019). Smarca4 Redirects Binding Of Macrophage Activating Transcription Factor 1 (Atf1) From Genes For Inflammation Resolution To Genes For Erythrocyte Resolution. Atherosclerosis, 287, e78.

Hao, Q., Zhao, X., Zhang, Y., Dong, Z., Hu, T., & Chen, P. (2017). Targeting overexpressed activating transcription factor 1 (ATF1) inhibits proliferation and migration and enhances sensitivity to paclitaxel in esophageal cancer cells. Medical science monitor basic research, 23, 304.

Shi, Y., Wang, W., Yang, B., & Tian, H. (2017). ATF1 and RAS in exosomes are potential clinical diagnostic markers for cervical cancer. Cell biochemistry and function, 35(7), 477-483.

Salat-Canela, C., Paulo, E., Sánchez-Mir, L., Carmona, M., Ayté, J., Oliva, B., & Hidalgo, E. (2017). Deciphering the role of the signal-and Sty1 kinase-dependent phosphorylation of the stress-responsive transcription factor Atf1 on gene activation. Journal of Biological Chemistry, 292(33), 13635-13644.

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For research use only. Not intended for any clinical use.

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