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Mouse Anti-ASH1L Monoclonal Antibody (ASH5H03) (CBMAB-1372-YC)


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Published Data
Mouse Anti-ASH1L Monoclonal Antibody (ASH5H03) CBMAB-1372-YC in WB
Western blot analysis of ASH1L protein levels in different cancer cell lines. The GAPDH was used as a loading control. ...View More
Yu, M., Jia, Y., Ma, Z., Ji, D., Wang, C., Liang, Y., ... & Zeng, L. (2022). Structural insight into ASH1L PHD finger recognizing methylated histone H3K4 and promoting cell growth in prostate cancer. Frontiers in Oncology, 12, 906807.

Summary

Host Animal
Mouse
Specificity
Human
Clone
ASH5H03
Antibody Isotype
IgG1, κ
Application
IP, WB

Basic Information

Immunogen
Amino acids 1611-1728 of ASH1L of human origin.
Specificity
Human
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.1 mg/ml
Purity
>95%, as determined by SDS-PAGE analysis
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
ash1 (absent, small, or homeotic)-like (Drosophila)
Introduction
ASH1L is a member of the trithorax group of transcriptional activators which contains four AT hooks, a SET domain, a PHD-finger motif, and a bromodomain. ASH1L is localized to many small speckles in the nucleus, and also to cell-cell tight junctions.
Entrez Gene ID
55870
UniProt ID
Q9NR48
Alternative Names
ASH1; KMT2H; MRD52; ASH1L1
Function
Histone methyltransferase specifically trimethylating 'Lys-36' of histone H3 forming H3K36me3 (PubMed:21239497).
Also monomethylates 'Lys-9' of histone H3 (H3K9me1) in vitro (By similarity).
The physiological significance of the H3K9me1 activity is unclear (By similarity).
Biological Process
Decidualization Source: Ensembl
Flagellated sperm motility Source: Ensembl
Histone H3-K36 dimethylation Source: HGNC
Negative regulation of acute inflammatory response Source: Ensembl
Negative regulation of I-kappaB kinase/NF-kappaB signaling Source: Ensembl
Negative regulation of MAPK cascade Source: Ensembl
Positive regulation of transcription by RNA polymerase II Source: Ensembl
Post-embryonic development Source: Ensembl
Single fertilization Source: Ensembl
Skeletal system development Source: Ensembl
Tarsal gland development Source: Ensembl
Uterine gland development Source: Ensembl
Uterus morphogenesis Source: Ensembl
Cellular Location
Nucleus; Tight junction; Chromosome. The relevance of tight junction localization is however unclear.
Involvement in disease
Mental retardation, autosomal dominant 52 (MRD52): A form of mental retardation, a disorder characterized by significantly below average general intellectual functioning associated with impairments in adaptive behavior and manifested during the developmental period.
PTM
Methylated at Gln-1220 by N6AMT1.
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For research use only. Not intended for any clinical use.

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